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Standard rodent chow

Manufactured by Altromin
Sourced in Germany

Standard rodent chow is a laboratory animal feed product designed to provide a complete and balanced diet for rodents used in research. It is formulated to meet the nutritional requirements of various rodent species, including mice and rats, and is intended to support their health and well-being during scientific investigations.

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8 protocols using Standard rodent chow

Male mice of the congenic B6.Cg-Tg(SOD1*G93A)1Gur/J line were purchased from JAX (Strain #: 004435, The Jackson Laboratory, Bar Harbor, ME, USA) and bred in-house with C57BL/6J females obtained from CRIVER (Strain Code 632, Charles River Laboratories, Sulzfeld, Germany). Each mouse was genotyped using RT-PCR with DNA extracted from ear tissue collected at P21. The SOD1 copy number was checked prior to the study start to ensure a stable phenotype progression as previously described [17 (link)].
The mice were housed at the animal facility of the Forschungszentrum Jülich under a controlled specific-pathogen-free (SPF) environment (12/12 h light/dark cycle, humidity maintained around 50%, and a room temperature between 20 and 23 °C). Food, water, and cages were autoclaved prior to entering the SPF area. A maximum of five SOD1*G93A mice and non-transgenic littermates were housed in individually ventilated cages on standardized rodent bedding (Rettenmaier, Rosenberg, Germany). Dried, pelleted standard rodent chow (Altromin, Lage, Germany) as well as tap water were available for the animals ad libitum. After disease onset, nutrition of the animals was ensured using gel pads (Solid Drink® SDST-75, Triple A trading, Tiel, The Netherlands).
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Male Sprague–Dawley (SD) rats (n = 38, 56–82 g) were obtained from 13 litters (Charles River Laboratories). Animals were separated from their mothers on postnatal day (PND) 21. Four animals (two pairs of different experimental groups) were housed together in Makrolon Type IV open cages under controlled environmental conditions (22 ± 2 °C; 55 ± 10% humidity; 10/14-h dark/light cycle with lights on at 06:00 a.m.). Until a bodyweight of 200 g, animals were fed ab libitum with standard rodent chow (Altromin, Lage, Germany). Then, 14-g chow was fed per day and animal. Animals always had access to tap water. To ensure the well-being of all animals, bodyweight and clinical scores were assessed regularly, at least two times per week.
All experiments were carried out per the EU directive 2010/63 and were approved by the local animal ethics committee (Lower Saxony State Office for Consumer Protection and Food Safety; AZ 17/2583). All efforts were made to minimize the number of animals used and their suffering.
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All animals were treated in accordance with the guidelines of EU Directive 2010/63/EU for animal experiments and were approved by the Animal Care Committee of Rhineland-Palatinate, Germany. Mice deficient in the gene coding for apolipoprotein E (ApoE-/-) and age-matched wild-type controls (C57BL/6J) were obtained from The Jackson Laboratory, Bar Harbour, ME, USA. Male mice were fed with a standard rodent chow (Altromin, Lage, Germany) and used for experiments at the age of 12 months. In a previous study using mice from our mouse stock and the same chow, plasma low-density lipoprotein (LDL) and total cholesterol levels were increased by more than 5-fold in 6-month-old ApoE-/- mice compared to wild-type controls [21 (link)]. Mice were housed under standardized conditions (12 hours light/dark cycle, temperature of 22 ± 2°C, humidity of 55 ± 10%, and free access to food and tap water).
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All experiments were performed according to the protocol approved by the Landesamt für Naturschutz, Umweltschutz und Verbraucherschutz (LANUV, AZ: 84-02.05.50.A15.005), Northrhine-Westphalia, Germany according to the legislation for the protection of animals used for scientific purposes (Directive 2010/63/EU). C57Bl/6 Syndecan-1-/- (Sdc1−/−) mice were generated as a global knockout by Stepp et al.26 (link),33 (link). Briefly, the signal sequence, exon 1 and a large part of the first intron of the Sdc1 gene was interrupted by a neomycin resistance cassette driven by a PGK promotor. All mice were inbred homozygously at a C57BL/6 genetic background. Mice were kept under conventional not pathogen-free conditions with a 12 h light/dark cycle, up to 65% relative humidity and 20 °C temperature in open cages grouped with up to 4 animals enriched with nesting material and paper houses. Mice had access to tap water and standard rodent chow (Altromin GmbH, Lage, Germany) ad libitum. Syndecan-1 deficient mice showed no severe phenotype, are vital and fertile.
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The national animal experiments inspectorate approved the study (number 2013−15−2934−00810). Standard housing conditions and husbandry practices were identical across the control and experimental groups. Adult male rats, Rattus norvegicus, Wistar (250–350 g) were supplied by Janiver Labs (Le Genest-Saint-Isle, France) and housed pairwise in cages, an artificial 12 h:12 h light-dark cycle and controlled temperature and humidity (21 ± 2°C and 55 ± 2%). The rats had free access to tap water and standard rodent chow (Altromin, Lage, Germany). The rats were cared for daily and monitored for pain and distress between and after the procedures in accordance to the general distress scoring sheet from Wolfensohn et al [29 ]. The rats were allowed to acclimate at least 1 week prior to the surgical procedures.
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All experimental procedures were conducted in compliance with ethical standards and received prior approval from the Animal Care Committee of Rhineland-Palatinate, Germany, under approval number 23 177-07/G 13-1-034. The experiments strictly adhered to the guidelines outlined in the EU Directive 2010/63/EU for animal experiments.
Mice deficient in the gene encoding apolipoprotein E (ApoE−/−) and wild-type controls (C57BL/6J) were procured from The Jackson Laboratory, located in Bar Harbor, ME, USA. Male mice, aged 12 months, were selected for the experiments. These mice were maintained on a standard rodent chow (Altromin, Lage, Germany). The mice were housed in a carefully controlled environment, featuring a 12 h light/dark cycle, a constant temperature of 22 ± 2 °C, humidity levels maintained at 55 ± 10%, and provided with unrestricted access to both food and tap water.
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Adult male Sprague-Dawley rats (Charles River Laboratories; n = 26) were housed in groups of two to four animals per Makrolon Type IV open cages under controlled environmental conditions (22 ± 2°C; 55 ± 10% humidity; 10/14 h dark/light cycle with lights on at 06:00 a.m.). Tap water was freely available and standard rodent chow (Altromin, Lage, Germany) was fed with 14–16 g per day and animal to enhance saliency of reward pellets while ensuring weekly weight gain of about 5–15%. Body weight and clinical scores were assessed at least two times a week to ensure the well-being of the rats.
All experiments were carried out in accordance with the EU directive 2010/63 and were approved by the local animal ethics committee (Lower Saxony State Office for Consumer Protection and Food Safety, AZ 18/2874). All efforts were made to minimize the number of animals and their suffering.
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Male Apoe knockout (Apoe KO) mice B6.129P2-Apoetm1Unc N11 were obtained from Taconic (Borup, Denmark). The mouse model was originally developed through embryonic transfer51 (link) and the line is maintained by inbreeding homozygous mice. As control mice, male wild type (WT) C57BL/6JBomTac mice were used with same genetic background (C57BL/6) as the Apoe KO mice. The animals (WT N = 48, Apoe KO N = 48) were purchased at 10 weeks of age, group housed, and kept on a 12-h light/dark cycle at constant room temperature. Both WT and Apoe KO mice were feed ad libitum water and standard rodent chow (#131003, Altromin, Lage, Germany).
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