For rapid COG4 degradation, a stock solution of 0.5 M Indole-3-acetic acid sodium salt (auxin, IAA, Sigma # I5148) was prepared in water and stored in a frozen aliquot. Time course treatment of cells was performed with 500 μM IAA for 0.5, 1, 2, 24, and 48 h at 37°C. The cells without auxin treatment were considered as untreated control.
Cf 0500 a
The CF-0500-A is a laboratory centrifuge that can achieve a maximum speed of 5,000 rpm. It has a rotor capacity of up to 6 samples. The centrifuge is designed for general-purpose centrifugation applications in a research or clinical laboratory setting.
3 protocols using cf 0500 a
Rapid Degradation of COG4 in RPE1 and HEK293T Cells
hTERT RPE1 and HEK293T Cell Culture
Rapid COG4 Degradation in RPE1 Cells
For rapid COG4 degradation, a stock solution of 0.5 M Indole‐3‐acetic acid sodium salt (auxin, IAA, Sigma # I5148) was prepared in water and stored in a frozen aliquot. Time course treatment of cells was performed with 500 μM IAA for 0.5, 1, 2, 24, and 48 h at 37°C. The cells without auxin treatment were considered as untreated control.
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