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Cf 0500 a

Manufactured by Atlas Biologicals

The CF-0500-A is a laboratory centrifuge that can achieve a maximum speed of 5,000 rpm. It has a rotor capacity of up to 6 samples. The centrifuge is designed for general-purpose centrifugation applications in a research or clinical laboratory setting.

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3 protocols using cf 0500 a

1

Rapid Degradation of COG4 in RPE1 and HEK293T Cells

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hTERT RPE1 (retinal pigment epithelial) and HEK293T cells were purchased from ATCC. hTERT RPE1 COG4 KO cells were described previously.29 (link) Cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) containing Nutrient mixture F-12 (DMEM/F12, Corning 10–092-CV) supplemented with 10% fetal bovine serum (Atlas Biologicals, CF-0500-A). Cells were incubated in a 37°C incubator with 5% CO2 and 90% humidity.
For rapid COG4 degradation, a stock solution of 0.5 M Indole-3-acetic acid sodium salt (auxin, IAA, Sigma # I5148) was prepared in water and stored in a frozen aliquot. Time course treatment of cells was performed with 500 μM IAA for 0.5, 1, 2, 24, and 48 h at 37°C. The cells without auxin treatment were considered as untreated control.
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2

hTERT RPE1 and HEK293T Cell Culture

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hTERT RPE1 (Retinal Pigment Epithelial) and HEK293T cells (a human cell line obtained from embryonic kidney but exhibiting properties of immature neurons) purchased from ATCC were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) containing Nutrient mixture F-12 (Corning 10–092-CV) supplemented with 10% Fetal Bovine Serum (Atlas Biologicals, CF-0500-A). Cells were incubated in a 37°C incubator with 5% CO2 and 90% humidity.
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3

Rapid COG4 Degradation in RPE1 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
hTERT RPE1 (retinal pigment epithelial) and HEK293T cells were purchased from ATCC. hTERT RPE1 COG4 KO cells were described previously.29 Cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) containing Nutrient mixture F‐12 (DMEM/F12, Corning 10–092‐CV) supplemented with 10% fetal bovine serum (Atlas Biologicals, CF‐0500‐A). Cells were incubated in a 37°C incubator with 5% CO2 and 90% humidity.
For rapid COG4 degradation, a stock solution of 0.5 M Indole‐3‐acetic acid sodium salt (auxin, IAA, Sigma # I5148) was prepared in water and stored in a frozen aliquot. Time course treatment of cells was performed with 500 μM IAA for 0.5, 1, 2, 24, and 48 h at 37°C. The cells without auxin treatment were considered as untreated control.
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