Cell lysates were prepared as described previously16 (link). Antibodies directed against E2F1 (KH-95), CD44 (Sp37) and CD24 (C-20) were purchased from Santa Cruz (Santa Cruz, CA). While, E-cadherin (24E10), EpCAM (UV1D9), Cyclin D1 (2922), BMI-1, ERK1/2 (137F5), phospho-ERK1/2 (Thr202/Tyr204) (20G11), MMP-9, ALDH-1, Akt, phospho-Akt (Ser473)(193H12), JAK2, phosphor-JAK2 (Tyr1007/1008), Nanog, Snail (C15D3), FAP-a and Glyceraldehydes-3-phosphate dehydrogenase (GAPDH, FL-335), NF-κB p65 [D14E12], and pNF-κB p65 (Ser536) from Cell Signaling (Danvers, MA). In addition, AUF1 (ab50692), alpha smooth muscle actin (α-SMA), Stromal- derived factor-1 (SDF-1), transforming growth factor beta 1 (TGF-β1) (2Ar2), Twist-1, Vimentin (RV202), Ki-67, N-cadherin, and interleukin-6 (IL-6) were purchased from Abcam (Cambridge, MA); Anti-Rabbit IgG HRP Conjugate (W401), anti-mouse IgG HRP Conjugate (W402) and donkey anti-Goat IgG (H + L), HRP Conjugate (V805) were purchased from Promega (USA).
Mmp 9
Manufactured by Cell Signaling Technology
Sourced in United States, China, United Kingdom, Germany, Japan
About the product
MMP-9 (Matrix Metalloproteinase-9) is a lab equipment product offered by Cell Signaling Technology. MMP-9 is an enzyme involved in the degradation of the extracellular matrix. It plays a role in various physiological and pathological processes.
Automatically generated - may contain errors
Lab products found in correlation
Mmp 2, by Cell Signaling Technology (276 mentions)
β actin, by Cell Signaling Technology (128 mentions)
E cadherin, by Cell Signaling Technology (126 mentions)
Vimentin, by Cell Signaling Technology (109 mentions)
Gapdh, by Cell Signaling Technology (105 mentions)
Bcl 2, by Cell Signaling Technology (87 mentions)
N cadherin, by Cell Signaling Technology (86 mentions)
Pvdf membrane, by Merck Group (67 mentions)
P akt, by Cell Signaling Technology (66 mentions)
Cleaved caspase 3, by Cell Signaling Technology (56 mentions)
Bcl2 associated x (bax), by Cell Signaling Technology (51 mentions)
Cyclin d1, by Cell Signaling Technology (48 mentions)
Snail, by Cell Signaling Technology (48 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (47 mentions)
β catenin, by Cell Signaling Technology (42 mentions)
P erk, by Cell Signaling Technology (39 mentions)
Caspase 3, by Cell Signaling Technology (35 mentions)
Erk1 2, by Cell Signaling Technology (30 mentions)
P jnk, by Cell Signaling Technology (28 mentions)
P erk1 2, by Cell Signaling Technology (27 mentions)
Check compatibility
Market Availability & Pricing
Cell Signaling Technology offers several MMP-9 antibodies, including:
- **MMP-9 (D6O3H) XP® Rabbit mAb**: This commercialized product is available in 20 μl and 100 μl sizes.
- **MMP-9 (G657) Antibody**: This commercialized product is available in a 100 μl size.
- **MMP-9 (E3D1W) Rabbit mAb**: This commercialized product is available in a 100 μl size.
Pricing for these antibodies varies depending on the specific product and size. For example, the MMP-9 (D6O3H) XP® Rabbit mAb (20 μl) is listed at $186.20, while the 100 μl size is priced at $445.90 from third-party distributors.
Please refer to Cell Signaling Technology's official website or contact authorized distributors for the most accurate and up-to-date pricing information.
Need Operating Instructions, SDS, or distributor details? Just ask our AI Agent.
Is this product still available?
Get pricing insights and sourcing optionsSpelling variants (same manufacturer)
Similar products (other manufacturers)
Matrix metalloproteinase (MMP)-9 (by Abcam) - 13 citations
MMP-9 (by Agilent Technologies) - 8 citations
MMP-9 (by AnaSpec) - 8 citations
MMP-9 (by GE Healthcare) - 6 citations
MMP-9 (by Cytiva) - 6 citations
MMP-9 (by Oncogene) - 6 citations
MMP-2/MMP-9 inhibitor II (by Merck Group) - 5 citations
MMP-9 inhibitor (by Santa Cruz Biotechnology) - 4 citations
MMP-9 inhibitor screening assay kit (by Abcam) - 4 citations
MMP-9 (by Lab Vision) - 3 citations
The spelling variants listed below correspond to different ways the product may be referred to in scientific literature.
These variants have been automatically detected by our extraction engine, which groups similar formulations based on semantic similarity.
Product FAQ
717 protocols using «mmp 9»
1
Antibody Immunoblotting for Cell Signaling
2
Mitochondrial Dynamics in Colorectal Cancer
HCT116 colorectal cancer cells were obtained from American Type Culture Collection (Manassas, VA, USA). DMEM/Ham’s F-12 media, penicillin and streptomycin were purchased from Gibco (Thermo Fisher Scientific). Mdivi.1 was purchased from Santa Cruz biotechnologies. Inc. (Waltham, MA). MitoTracker Deep Red was purchased from Invitrogen (Carlsbad, MA USA). TMRM (tetramethylrhodamine methyl ester) was purchased from Abcam (USA). DMSO and Crystal violet were purchased from Sigma-Aldrich (St. Louis, MO, USA). MTT was purchased from Bio Basic Inc. (Markham, Canada). Antibodies against Drp, p-Drp, Mfn2, AMPK-α, p-PAMK-α, Cox-2, iNos, MMP9 and β-actin were from Cell Signaling Technology (Danvers, MA). All other chemicals used were of scientific standard and from commercial sources.
3
Inhibiting PDE4, PKA, and UAE in Cell Study
Rolipram (a PDE4 inhibitor) was purchased from Sigma–Aldrich (catalog no.: R6520) and dissolved in ethanol to prepare a stock solution of 10 mM. The selective PKA inhibitor H89 was also procured from Sigma–Aldrich (catalog no.: B1427) and dissolved in dimethyl sulfoxide (DMSO) to prepare a stock solution of 1 mM. Inhibitor of UAE, TAK-243, was obtained from Cayman Chemical Company (catalog no.: 30108) and dissolved in DMSO to prepare a stock solution of 100 μM. The primary antibodies used in this study are PKA (Santa Cruz Biotechnology; catalog no.: sc-28315, Research Resource Identifier [RRID]: AB_628136), PTEN (Cell Signaling Technology; catalog no.: 9552, RRID: AB_10694066), PI3K (Cell Signaling Technology; catalog no.: 4249, RRID: AB_2165248), Akt (Cell Signaling Technology; catalog no.: 9272 [also 9272S], RRID: AB_329827), p-GSK3β (Santa Cruz Biotechnology; catalog no.: sc-373800, RRID: AB_10920410), GLI1 (Novus Biologicals; catalog no.: NB600-600, RRID: AB_2111758), GLI2 (Novus Biologicals; catalog no.: NB600-874, RRID: AB_10001953), GLI3 (Novus Biologicals; catalog no.: NBP2-29627), anti-HA (Cell Signaling Technology; catalog no.: 2367, RRID: AB_10691311), MMP2 (Cell Signaling Technology; catalog no.: 4022, RRID: AB_2266622), MMP9 (Cell Signaling Technology; catalog no.: 3852, RRID: AB_2144868), E-cadherin (Cell Signaling Technology; catalog no.: 14472, RRID: AB_2728770), and vimentin (Cell Signaling Technology; catalog no.: 3932 [also 3932S], RRID: AB_2288553). The secondary antibodies used in this study are goat antimouse IgG–horseradish peroxidase (HRP) (Santa Cruz Biotechnology; catalog no.: sc-2005, RRID: AB_631736) and goat anti-rabbit IgG-HRP (Santa Cruz Biotechnology; catalog no.: sc-2004, RRID: AB_631746).
4
Western Blot Analysis of Protein Expression
Thirty micrograms of protein samples were loaded onto an SDS‐PAGE gel (Invitrogen, CA, USA). After transferring the proteins to a nitrocellulose blotting membrane (GE Healthcare, TX, USA), blocking was performed using a 5% (w/v) bovine serum albumin (BSA) solution (Capricorn Scientific, Hessen, Germany) for 2 h at room temperature to prevent non‐specific binding. Subsequently, the membranes were subjected to overnight incubation at 4°C with primary antibodies. Primary antibodies included β‐actin (RRID: AB_476693; #A2066) was purchased from Sigma‐Aldrich (Sigma‐Aldrich, MO, USA), MMP‐9 (RRID: AB_2144612; #2270), and mTOR (RRID: AB_2105622; #2983) were purchased from Cell Signaling Technology Inc. (Cell Signaling Technology, MA, USA). Phospho‐p44/42 MAPK (Erk1/2) (RRID: AB_331646; #9101), Phospho‐mTOR (RRID: AB_10691552; #5536), and Phospho‐Akt (Ser473) (RRID: AB_2315049; #4060) were kindly provided by Rutaiwan Tohtong and Thaned Kangsamaksin, respectively, at Mahidol University. A secondary antibody, anti‐rabbit IgG conjugated with horseradish peroxidase (RRID: AB_2099233; #7074) from Cell Signaling Technology Inc. (Cell Signaling Technology, MA, USA) was used for detection. To enhance the signal of protein expression, an enhanced chemiluminescence (ECL) substrate (Millipore, MA, USA) was employed. The protein expression was then visualized using the Azure 600 imaging system (Azure Biosystems, CA, USA) and analyzed by ImageJ software (National Institutes of Health, MD, USA).
5
Western Blot Analysis of Protein Expression
The cells were lysed in a buffer composed of 50 mM Tris, 5 mM EDTA, 150 mM NaCl, 1 mM dithiothreitol, 0.01% NP-40, and 0.2 mM phenylmethylsulfonyl fluoride. Protein concentrations in the lysates were determined using bovine serum albumin solutions of known concentrations as standards. Equal amounts of protein from each sample were separated based on molecular weight by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Following electrophoresis, proteins were transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA) via electrophoretic transfer. The membranes were blocked with 5% (w/v) nonfat dry milk in Tris-buffered saline containing Tween-20 (TBS-T) for 1 h at room temperature. Subsequently, the membranes were incubated overnight at 4°C with primary antibodies to SERPINB2 (Cat. No: ab47742; Abcam), MMP-2 (Cat. #4022; Cell Signaling Technology, Beverly, MA, USA), MMP-9 (Cat. #13667; Cell Signaling Technology), caspase-3 (Cat No. #9662; Cell Signaling Technology), and β-actin (Cat No.: ab189073; Abcam). After incubation with the primary antibody, the membranes were washed and incubated with horseradish peroxidase-conjugated secondary antibodies, including goat anti-rabbit IgG (554021; BD Pharmingen, San Diego, CA, USA) and goat anti-mouse IgG (554002; BD Pharmingen), for 60 min at room temperature. The bound antibodies were visualized using an enhanced chemiluminescence reagent.
Top 5 protocols citing «mmp 9»
1
Immunoblotting Analysis of Prostate Samples
2
Western Blot Analysis of Protein Expression
Antibodies against the following proteins were used: PinX1 (1:200 for WB, 1:50 for IHC; Novus Biologicals); MMP-2 (1:100 for WB; Cell Signaling Technology, Beverly, MA, USA); MMP-2 (1:50 for IHC; Santa Cruz); MMP-9 (1:200 for WB, Cell Signaling Technology); TIMP-1(1:200 for WB, Santa Cruz); TIMP-2 (1:200 for WB, 1:100 for IHC; Santa Cruz); NF-κB-p65 (1:500 for WB, 1:200 for IHC; Santa Cruz); β-actin (1:1000 for WB; Cell Signaling Technology); Infrared IRDye-labeled secondary antibody (1:10000; LI-COR, Lincoln, NE, USA) was applied to the blot for 1 hour at room temperature. The signals were detected with Odyssey Infrared Imaging system (LI-COR).
Western blot analysis was performed as described previously [56 (link)]. Cells were harvested and washed twice with PBS. Whole-cell proteins were extracted as described previously. Protein concentrations were determined by protein assay (Bio-Rad). All protein samples were denatured, electrophoresed on SDS/polyacrylamide gels and transferred onto polyvinylidene difluoride membranes (Millipore).
Western blot analysis was performed as described previously [56 (link)]. Cells were harvested and washed twice with PBS. Whole-cell proteins were extracted as described previously. Protein concentrations were determined by protein assay (Bio-Rad). All protein samples were denatured, electrophoresed on SDS/polyacrylamide gels and transferred onto polyvinylidene difluoride membranes (Millipore).
3
Western Blotting Analysis of Cell Signaling Proteins
Western blotting was performed using a SDS-PAGE Electrophoresis System according to the previous39 (link), 40 (link) description with rabbit polyclonal anti-FAP antibody (1 : 1000; LifeSpan BioSciences Inc., Seattle, WA, USA), anti-β-actin, CCND1, CDK4, p21, E2F1, and p27 (1 : 500; Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-C-Myc, PTEN, β-catenin, GSK-3b, p-GSK-3b(ser-9), p-Erk1/2(Tyr202/Tyr204),Erk1/2, p-MEK1/2 (Ser-217/221), MEK1,CCNE1, MMP2, MMP9, p-RB (ser780), RB, AKT, p-Akt (Ser-473), PI3K, p-PI3K (Tyr458), p16, p27, Slug, Snail, Vimentin, N-cadherin, and E-cadherin (1 : 1000; Cell Signaling Technology, Danvers, MA, USA). Signals were detected using enhanced chemiluminescence reagents (Pierce, Rockford, IL, USA).
4
Comprehensive Immunoblotting Analysis of Signaling Pathways
5
Comprehensive Protein Analysis of IL-32γ Mice
Skin tissues from WT mice and IL-32γ mice were lysed by Pro-prep protein extraction buffer (iNtRON, Sungnam, Korea) and the total protein concentration was determined using the Bradford reagent (Bio-Rad, Hercules, CA, USA). Nuclear extraction was performed using nuclear extraction kit (Abcam, Cambridge, MA, USA). The membranes were immunoblotted with specific primary antibodies. The intensity of the bands was measured using the Fusion FX 7 image acquisition system (Vilber Lourmat, Eberhardzell, Germany). Specific primary antibodies were purchased from Santa Cruz Bio (p-IKKα/β, IKKα/β, p-JNK, JNK, p-ERK, p-p38, p38, p-STAT3, STAT3, p50, Histone H1, Cyclin D1, CDK4, Bax, Bcl-2, MMP-2, MMP-9 and β-actin; Dallas, TX, USA), Cell Signaling Technology (Myc-tag and ERK; Trask Lane, Danvers, MA, USA), Abnova (CD133; Taipei, Taiwan), Novus Biologicals (TIMP-1, iNOS and COX-2; Littleton, CO, USA) and Abcam (CD44, ITGAV, S100A8 and p65; Cambridge, MA, USA). β-actin and Histone H1 was used as a loading control.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!
Request a quote
🧪 Need help with an experiment or choosing lab equipment?
I search the PubCompare platform for you—tapping into 40+ million protocols to bring you relevant answers from scientific literature and vendor data.
I search the PubCompare platform for you—tapping into 40+ million protocols to bring you relevant answers from scientific literature and vendor data.
1. Find protocols
2. Find best products for an experiment
3. Validate product use from papers
4. Check Product Compatibility
5. Ask a technical question
Want to copy this response? Upgrade to Premium to unlock copy/paste and export options.