BDNF
BDNF is a laboratory reagent that functions as a neurotrophin, a type of protein that supports the growth, maturation, and survival of neurons. It plays a crucial role in the development and maintenance of the nervous system.
Lab products found in correlation
23 protocols using BDNF
BDNF-Induced Protein Analysis in DIV Neurons
Neuroinflammation and Neurodegeneration Assays
GC Migration Assay in Neurodegeneration
A Boyden chamber cell migration assay was performed as described in [32 (link)]. Briefly, purified GCs in serum-free Dulbecco’s Modified Eagle Medium (DMEM) were placed in the poly-lysine precoated upper Boyden chamber while BDNF (40 ng/mL, #B-250, Alomone Labs, Jerusalem, Israel) was added to the lower chambers in the same medium. After overnight incubation at 37°C in 5% CO2, the upper surface of the membranes was scraped free of cells and debris. The membranes and covers were washed, fixed, and stained with Hoechst (Hoechst-33258, Invitrogen, Milan, Italy). Cells adhering to the underside of the membrane were counted under an epifluorescence Zeiss microscope with a 10× objective. The total number of cells that had migrated was calculated in five adjacent fields and the average values of each experimental group were expressed in arbitrary units, normalizing to the untreated control.
Hippocampal Protein Analysis in Rats
BDNF and Mirtazapine Neuronal Effects
Intracerebral Infusion Protocols for Memory Modulation
Primary Spinal Cord Neuron Isolation
Hippocampal Protein Expression Analysis
Neuronal Induction of iPSCs
BDNF Stimulation of Hippocampal Neurons
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