Human HT1080 cells were purchased from the American Type Culture Collection. HT1080 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Thermo Fisher Scientific) supplemented with 10% (v/v) fetal bovine serum (FBS; Thermo Fisher Scientific), penicillin (100 U/ml), and streptomycin (100 μg/ml) (Thermo Fisher Scientific). RS4;11 cells were purchased from the German Collection of Microorganisms and Cell Cultures (DSMZ) and cultured in α-MEM with ribo- and deoxyribonucleosides (Thermo Fisher Scientific) supplemented with 10% (v/v) FBS (Thermo Fisher Scientific), penicillin (100 U/ml), and streptomycin (100 μg/ml) (Thermo Fisher Scientific). Jurkat T cells were provided by A. Rösen-Wolff and cultured in RPMI 1640 medium (Thermo Fisher Scientific) supplemented with 10% (v/v) FBS (Thermo Fisher Scientific), penicillin (100 U/ml), and streptomycin (100 μg/ml) (Thermo Fisher Scientific). Murine thymocytes were isolated as described below and cultured in RPMI 1640 medium (Thermo Fisher Scientific) supplemented with 10% (v/v) FBS (Thermo Fisher Scientific), penicillin (100 U/ml), and streptomycin (100 μg/ml) (Thermo Fisher Scientific). All cells were cultured in a humidified 5% CO2 atmosphere.
Streptomycin
Manufactured by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, China, France, Canada, Australia, Japan, Switzerland, Italy, Belgium, Israel, Austria, Spain, Netherlands, Poland, Brazil, Denmark, Argentina, Sweden, New Zealand, Ireland, India, Gabon, Macao, Portugal, Czechia, Singapore, Norway, Thailand, Uruguay, Moldova, Republic of, Finland, Panama
Streptomycin is a broad-spectrum antibiotic used in laboratory settings. It functions as a protein synthesis inhibitor, targeting the 30S subunit of bacterial ribosomes, which plays a crucial role in the translation of genetic information into proteins. Streptomycin is commonly used in microbiological research and applications that require selective inhibition of bacterial growth.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin, by Thermo Fisher Scientific (34640 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (12301 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (5483 mentions)
L glutamine, by Thermo Fisher Scientific (3933 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2887 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1785 mentions)
Glutamax, by Thermo Fisher Scientific (1179 mentions)
Dmem f12, by Thermo Fisher Scientific (993 mentions)
Fetal bovine serum (fbs), by Merck Group (972 mentions)
Trypsin edta, by Thermo Fisher Scientific (863 mentions)
Amphotericin b, by Thermo Fisher Scientific (842 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (818 mentions)
Glutamine, by Thermo Fisher Scientific (784 mentions)
Penicillin g, by Thermo Fisher Scientific (742 mentions)
Sodium pyruvate, by Thermo Fisher Scientific (726 mentions)
Fetal calf serum (fcs), by Thermo Fisher Scientific (724 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (711 mentions)
Non essential amino acids (neaa), by Thermo Fisher Scientific (689 mentions)
Trypsin, by Thermo Fisher Scientific (619 mentions)
Hepes, by Thermo Fisher Scientific (617 mentions)
24 872 protocols using streptomycin
1
Cultivation of Various Cell Lines
2
Cell Culture Conditions for Cancer Cell Lines
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DLD-1 and COLO-205 were purchased at ATCC and regularly tested for mycoplasma infection. Cells were grown in RPMI-1640 Medium (Sigma) supplemented with 10% (v/v) Fetal Bovine Serum (Gibco), 2 mM L-glutamine (Thermo Fisher), 100 Units/mL penicillin (Thermo Fisher) and 100 mg/mL streptomycin (Thermo Fisher). HCT116 cells were grown in McCoy's 5A Medium (Sigma) supplemented with 10% (v/v) Fetal Bovine Serum (Gibco), 2 mM L-glutamine (Thermo Fisher), 100 Units/mL penicillin (Thermo Fisher) and 100 mg/mL streptomycin (Thermo Fisher). LoVo cells were grown in Kaighn's Modification of Ham's F-12 Medium (Sigma) supplemented with 10% (v/v) Fetal Bovine Serum (Gibco), 2 mM L-glutamine (Thermo Fisher), 100 Units/mL penicillin (Thermo Fisher) and 100 mg/mL streptomycin (Thermo Fisher). The cells were grown at 37 °C in the presence of 5% CO2. HEK293T cells and CCD841 cells were grown in Dulbecco’s Modified Eagle’s Medium (Sigma) supplemented with 10% (v/v) Fetal Bovine Serum (Gibco) and 2 mM L-glutamine (Thermo Fisher) 100 Units/mL penicillin (Thermo Fisher) and 100 mg/mL streptomycin (Thermo Fisher. All the cells were grown at 37 °C in the presence of 5% CO2.
3
Cell Culture Conditions Across Cell Lines
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BeWo cells (RIKEN Cell Bank, code RCB1644) were grown in Ham's F-12 medium (17458-65, Nacalai Tesque, Inc.) supplemented with 100 unit/mL penicillin, 100 µg /mL streptomycin (15140-122, Thermo Fisher Scientific), and 10% fetal bovine serum (10437-010, Thermo Fisher Scientific) in an incubator with 5% CO2 at 37°C. HuH-7, VMRC-RCW, NEC14, HuO-3N1, and RAJI Cells (RIKEN Cell Bank, code RCB1942, RCB1963, RCB0490, RCB2104, and RCB1647) were grown in RPMI 1640 medium (30264-85, Nacalai Tesque, Inc.) supplemented with 100 unit/mL penicillin, 100 µg/mL streptomycin (15140-122, Thermo Fisher Scientific), and 10% fetal bovine serum (10437-010, Thermo Fisher Scientific) in an incubator with 5% CO2 at 37°C. MCF7 cells (RIKEN Cell Bank, code RCB1940) were grown in MEM (21442-25, Nacalai Tesque, Inc.) supplemented with 100 unit/mL penicillin, 100 µg /mL streptomycin (15140-122, Thermo Fisher Scientific), 0.1 mM nonessential amino acids (M71455, Sigma-Aldrich), 1 mM sodium pyruvate (S8636, Sigma-Aldrich) and 10% fetal bovine serum (10437-010, Thermo Fisher Scientific) in an incubator with 5% CO2 at 37°C. HeLa S3 cells (JCRB Cell Bank, JCRB0713) were grown in DMEM (08458-45, Nacalai Tesque) supplemented with 100 unit/mL penicillin, 100 µg/mL streptomycin (15140-122, Thermo Fisher Scientific) and 10% fetal bovine serum (10437-010, Thermo Fisher Scientific) in an incubator with 5% CO2 at 37°C.
4
Isogenic Tetraploid Cell Line Generation
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All breast cancer cell lines were authenticated by ATCC and used at early passage numbers. Isogenic tetraploid cell lines were generated as described6 (link). hTERT-RPE-1 were cultured in DME/F12 (HyClone) supplemented with 10% FBS (ThermoFisher) with 50 IU/mL penicillin and 50 μg/mL streptomycin (ThermoFisher). HCT116, CAMA-1, MDA-MB-415, MDA-MB-453, MDA-MB-134-VI, MDA-MB-157, Hs578T, MDA-MB-231, MDA-MB-361 cells were cultured in high glucose DMEM (Gibco) supplemented with 10% FBS with 50 IU/mL penicillin and 50 μg/mL streptomycin. ZR-75–30 and HCC1806 cells were cultured in RPMI (Gibco) supplemented with 10% FBS with 50 IU/mL penicillin and 50 μg/mL streptomycin. MCF10A cells were cultured in DME/F12 (HyClone) supplemented with 5% horse serum (ThermoFisher), 20ng/mL EGF (ThermoFisher), 500ng/mL hydrocortisone (ThermoFisher), 100ng/mL cholera toxin (Sigma), 10ug/ml insulin (ThermoFisher), with 50 IU/mL penicillin and 50 μg/mL streptomycin. RPE-1, HCT116, and MCF10A 2N and 4N cell lines were tested for mycoplasma contamination and confirmed to be negative.
5
Isogenic Tetraploid Cell Line Generation
6
Cell Culture Conditions for NSCLC Lines
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Human NSCLC H358, H1299, A549, H520, SK-MES-1, H1703 and normal human bronchial epithelial BEAS-2B cells were purchased from the China Infrastructure of Cell Line Resources. SK-MES-1 cells were cultured in Minimum Essential Medium (Thermo Fisher Scientific, Inc.) supplemented with 10% FBS (Gemini Bio Products), 10,000 U/ml penicillin and 10,000 μg/ml streptomycin (Thermo Fisher Scientific, Inc.). H520 cells were cultured in DMEM (Thermo Fisher Scientific, Inc.) supplemented with 10% FBS (Gemini Bio Products), 10,000 U/ml penicillin and 10,000 μg/ml streptomycin (Thermo Fisher Scientific, Inc.). The other cells were cultured in RPMI-1640 medium (Thermo Fisher Scientific, Inc.) supplemented with 10% FBS, 10,000 U/ml penicillin and 10,000 μg/ml streptomycin. BEAS-2B cells were cultured in Bronchial Epithelial Cell Medium (ScienCell Research Laboratories, Inc.) with 1% cell growth supplements (cat. no. 3962; ScienCell Research Laboratories, Inc.) and 1% penicillin/streptomycin (Thermo Fisher Scientific, Inc.). All cells were incubated at 37°C with 5% CO2.
7
Biotin Labeling and Autophagy Flux Analysis
8
Cell Culture Conditions for Various Cell Lines
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HEK 293TT cells, purchased from the National Cancer Institute’s Developmental Therapeutics Program (Frederick, Maryland, USA), were grown in complete DMEM (ThermoFisher) containing 10% FBS (ThermoFisher), 100 U/mL penicillin, 100 μg/mL streptomycin (Dutscher), 1x Glutamax-I (ThermoFisher) and 250 μg/mL Hygromycin B (Sigma-Aldrich). RS cells (Evercyte, Vienna, Austria) were grown in Optipro (ThermoFisher) containing 100 U/mL penicillin, 100 μg/mL streptomycin, 1x Glutamax-I. GM95 cells purchased from the RIKEN BRC cell bank were cultured in DMEM with 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin, and 1x Glutamax-I, and LNCaP cells were grown in RPMI medium supplemented with 10% of FBS, 100 U/mL penicillin, 100 μg/mL streptomycin, and 1x Glutamax-I. Cells were maintained at 37°C in a humidified 5% CO2 incubator, and passaged at confluence by trypsinization for 10 min with 1x TrypLE Express (ThermoFisher).
9
Cell Culture and Virus Propagation Methods
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Vero and VeroE6 (both African green monkey kidney origin) cells were grown at 37 °C and 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM) (Sigma-Aldrich, St. Louis, MO) containing 2–10% fetal bovine serum (FBS) (Wisent Inc., St. Bruno, Canada), 2 mM L-glutamine (Thermo Fisher Scientific, Waltham, MA), 50 U/mL penicillin (Thermo Fisher Scientific), and 50 μg/mL streptomycin (Thermo Fisher Scientific). BHK-T7 (baby hamster kidney) cells were grown at 37 °C and 5% CO2 in minimum essential medium (MEM) (Thermo Fisher Scientific) containing 10% tryptose phosphate broth (Thermo Fisher Scientific), 2% FBS (Wisent), 2 mM L-glutamine (Thermo Fisher Scientific), 50 U/mL penicillin (Thermo Fisher Scientific), and 50 μg/mL streptomycin (Thermo Fisher Scientific). C6/36 (Aedes albopictus) cells were grown at 32 °C and 5% CO2 in MEM (Thermo Fisher Scientific) containing 10% FBS (Wisent), non-essential amino acids solution (Thermo Fisher Scientific), 50 U/mL penicillin (Thermo Fisher Scientific), and 50 μg/mL streptomycin (Thermo Fisher Scientific). The ZIKV used for challenge was the French Polynesian strain propagated on C6/36 cells47 . Mouse-adapted EBOV was used for the challenge study in CD1 mice62 (link). VSV-EBOV was used as a control vaccine30 (link). The VSV-EBOV-Andes virus vaccine was used as the control vaccine in the time to immunity study63 (link).
10
Cell Culture Conditions and Protocols
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The HEK-293 (ATCC, CRL-1573) and HeLa (ATCC, CCL-2) cells were cultured in DMEM (4.5 g/L glucose, Thermo Fisher Scientific) supplemented with 2 mM GlutaMAX (Thermo Fisher Scientific), 10% FCS (Thermo Fisher Scientific), 100 U/mL of penicillin, and 100 µg/mL of streptomycin (Thermo Fisher Scientific). The PC12 Tet-Off cells (Clontech, 631134; PC12-TO) were maintained in DMEM medium (1 g/l glucose, Thermo Fisher Scientific) supplemented with 10% FCS, 5% horse serum (HS), 2 mM GlutaMAX, 100 U/mL penicillin, and 100 µg/mL streptomycin (all Thermo Fisher Scientific). The osteosarcoma U-2 OS cells (ATCC, HTB-96) were cultured in McCoy’s 5A (Modified) Medium supplemented with GlutaMAX containing 10% FCS, 100 U/mL penicillin, and 100 µg/mL streptomycin (all Thermo Fisher Scientific). The PC12-TO cells were grown on surfaces coated with poly-l -lysine (PLL, Sigma-Aldrich) for the maintenance and experiments. For coating, the plates were incubated with PLL (0.02 mg/mL final concentration diluted in ddH2O) for 30 min at 37 °C, washed twice with ddH2O, and air-dried. The cells were cultured at 37 °C and 5% CO2.
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