Anti-β-actin
Anti-β-actin is a primary antibody that specifically recognizes the beta-actin protein, a ubiquitous cytoskeletal protein found in eukaryotic cells. It is commonly used as a loading control in various protein analysis techniques, such as Western blotting, to normalize the expression levels of target proteins.
Lab products found in correlation
50 protocols using Anti-β-actin
Western Blot Antibody Validation
Western Blot Analysis of Protein Markers
Western Blot Analysis of Apoptosis Markers in HT22 Cells
Protein Extraction and Western Blotting
Quantification of MEX3C Protein Levels
Protein Expression Analysis in BV-2 Cells
Western Blot Analysis of Inflammatory Signaling
Western Blot Analysis of Spinal Cord Inflammation
The equal quantified proteins (50 μg) were denatured and loaded for separation by 10%–12% sodium dodecyl sulfate–polyacrylamide gel electrophoresis, then transferred to the polyvinylidene fluoride membrane (Millipore). Membranes were blocked with 5% nonfat milk/TBST for 2 h at room temperature and probed with the following primary antibodies: rabbit anti‐IL‐6, anti‐IL‐6Rα, anti‐JAK2, anti‐pJAK2, anti‐STAT3, anti‐pSTAT3, anti‐MIF, anti‐Cox‐2, and anti‐β‐actin (1:1000; Affinity). Subsequently, the membranes were incubated with 1:10,000 horseradish peroxidase‐conjugated goat anti‐rabbit secondary antibody (Affinity) in 5% nonfat milk/TBST for 1 h at room temperature. Finally, proteins were visualized with ECL reagent (Affinity) and analysed by ImageJ software (National Institutes of Health, Bethesda, MD, USA).
Protein Expression Analysis via Western Blotting
Carbon Monoxide Neuroprotection Protocol
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