The largest database of trusted experimental protocols

FlexA-200

Manufactured by Allsheng
Sourced in China

The FlexA-200 is a versatile laboratory equipment designed for general-purpose applications. It features a compact and durable construction, with a focus on reliable performance and ease of use. The core function of the FlexA-200 is to provide a stable and controlled environment for various laboratory tasks, ensuring consistent and accurate results.

Automatically generated - may contain errors

Lab products found in correlation

6 protocols using FlexA-200

Biofilm was determined as previous description (O’Toole 2011 (link)) with minor modification. V. splendidus AJ01 was reinoculated into fresh 2216E medium to make an initial bacterial cell suspension of 1.0 × 106 CFU/mL. The cell suspension was split into a 96-well polystyrene microplate. 2.5 μg/mL tryptanthrin was added as the experimental group, and the culture with the same volume of DMSO was used as the control sample. The plates were statically cultured in an incubator at 28 ℃ for 36 h, and then, the OD600 of the culture was measured. After that, biofilm was stained using crystal violet, and the absorbance at OD590 using a spectrophotometer (FlexA-200, Allsheng, China).
+ Open protocol
+ Expand
V. splendidus AJ01 was grown in a 2216E medium at 28 °C in filtered seawater containing 5 g/L of tryptone, 1 g/L of yeast extract, and 0.01 g/L of FePO4. To analyze the effect of L-Glu on the growth of V. splendidus AJ01, different concentrations of L-Glu were added to an M9 minimal medium with 3% salinity as the sole carbon source [26 (link)], and then the absorbance of the culture was recorded at 600 nm. To determine the growth of V. splendidus AJ01 in the coelomic fluid containing L-Glu, the filtrated and cell-free coelomic fluid at a ratio of 1% or 10% (v/v) was added into an M9 minimal medium with 3% salinity containing 10 mM L-Glu, respectively. The absorbance at 600 nm was measured with a Microplate Reader (FlexA-200, Allsheng, Hangzhou, China) under the above incubation conditions. Each growth was repeated in triplicate.
+ Open protocol
+ Expand
MIC of tryptanthrin was determined as described previously (Narendrakumar et al. 2019 (link)). Briefly, 2216E media supplemented with 0.5, 1, 2.5, 5, 10, 25, and 50 μg/mL tryptanthrin were used to culture V. splendidus AJ01. The control sample was the culture of V. splendidus AJ01 grown in medium without tryptanthrin. After being cultured for 24 h, OD600 was recorded using a microplate reader (FlexA-200, Allsheng, China). Each growth was performed and measured in triplicate.
+ Open protocol
+ Expand
Growth of bacterial isolates H1 and H2 was performed as described previously [[27] (link), [28] (link)]. A single colony was inoculated into 5 mL of 2216E and grown overnight at 28 °C under shaking condition. Overnight cultures were inoculated into 25 mL fresh 2216E in a sterilized glass conical flask at a ratio of 1:1000. The cultures were further incubated at 28 °C and the OD600 was measured at every 2 h interval for 24 h using a Microplate Reader (FlexA-200, Allsheng, Hangzhou, China).
+ Open protocol
+ Expand
When the pathogens challenge the host, REDOX stress and iron lack are the main environmental stresses that the pathogens need to conquer [31 (link),32 (link)]. Thus, H2O2 or 2,2′-dipyridyl (DP) were separately added into the medium to simulate oxidative stress and iron limited condition of the host. Briefly, overnight culture of bacterium H1 was inoculated into 2216E medium containing H2O2 at different concentrations of 0, 0.2, 0.4, 0.6, 0.8, 1 and 2 mM or DP at different concentrations of 0, 20, 40, 80, 160, 320 and 640 μM, respectively. After cultured for 12 h, 24 h, 36 h and 48 h, 100 μL of cultures were taken for the measurement of OD600 using Microplate Reader (FlexA-200, Allsheng, Hangzhou, China). The MIC of H2O2 or DP was defined as the lowest concentration at which no obvious growth was observed.
+ Open protocol
+ Expand
The antimicrobial activity of peptides was conducted in triplicate on separate occasions in 96-well flat-bottom plates using MIC and minimum bactericidal concentration (MBC) as previously described methods with slight modifications [15 ,25 ]. Briefly, Gram-positive bacteria (Staphylococcus aureus) and Gram-negative bacteria (E. coli, Vibrio harveyi, PDD,Pseudomonas aeruginosa, V. alginayticus, V. vulnificus, V. parahaemolyticus) were diluted with sterile PBS (pH 7.2). Synthesized AMPs were diluted to a series of concentrations with sterile PBS. Next, peptides were mixed separately with bacteria in equal volume, followed by incubation for 24 h at 26 °C or 37 °C in the dark. After the incubation period, samples were measured in terms of absorbance at 600 nm using a microplate reader (FlexA-200, ALLSHENG). Subsequently, 100 µL cultures were added on plates (TSA-1 or LB) in triplicate and incubated at the appropriate temperature overnight. Finally, colonies were counted and recorded. The MIC of AMPs was defined as the lowest concentration that completely inhibits bacterial growth, while the MBC was defined as the minimum AMP concentration without bacterial growth.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!