Hplc grade methanol

Acetonitrile and methanol (HPLC-grade) were purchased from Merck (Darmstadt, Germany). Sodium chloride (NaCl, analytical-grade), anhydrous magnesium sulfate (MgSO4, analytical-grade), and ammonium acetate (HPLC-grade) were purchased from ANPEL (Shanghai, China). Potato dextrose agar (PDA), octadecylsilane-bonded silica gel (C₁₈), and propylethylenediamine (PSA) were purchased from Wuzhi Biotechnology (Shanghai, China). The analytical standards (stock solutions) of AOH (100.0 µg/mL), AME (100.3 µg/mL), ALT (101.1 µg/mL), and TeA (101.1 µg/mL) dissolved in Acetonitrile were obtained from Romer Labs (Union, MO, USA). The analytical standard powders of citric acid (25 mg), malic acid (100 mg), tartaric acid (250 mg), frutose (250 mg), glucose (250 mg), and sucrose (200 mg) were purchased from ANPEL (Shanghai, China). The fixative for SEM (2.5% glutaraldehyde solution) was purchased from Solarbio Science & Technology (Beijing, China).

Cell culture media, supplements, consumables, dimethyl sulfoxide (DMSO; 99.9%), and formaldehyde solution were all acquired from Euroclone S.p.A. (Milan, Italy). Methanol (HPLC grade) was supplied by Merck KGaA (Darmstadt, Germany). Mitomycin C and cytochalasin B were obtained from D.B.A. Italia s.r.l. (Milan, Italy), while 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT) was sourced from Biosigma S.p.A. (Cona, Italy). Hoechst 33342 Staining Dye Solution from Abcam, supplied by Prodotti Gianni s.r.l. (Milano, Italy).

The standards of rutin, gallic acid, Folin–Ciocalteu phenol reagent, HPLC-grade methanol, 2,2-diphenyl-1-picrylhydrazyl, and ethanol were obtained from Sigma Aldrich (St. Louis, MO, USA). Stevioside and rebaudioside A were purchased from ChromaDex (Los Angeles, CA, USA). HPLC-grade acetonitrile and sodium acetate were obtained from Sharlau Chemie S. A. (Sentmenat, Spain). HCl, sodium carbonate, and acetic acid were purchased from Carl Roth (Karlsruhe, Germany). Hexamethylenetetramine and aluminum chloride were from Thermo Fisher Scientific (Lancashire, UK). The hydroponics media Hydro A and B were obtained from Plagron (Ospel, The Netherlands). All solutions were prepared with ultrapure 18.2 MΩ water from a Watek ultrapure water purification system (Watek Ltd., Ledeč nad Sázavou, Czech Republic).