Alp kit
The ALP kit is a laboratory equipment product designed for performing alkaline phosphatase (ALP) assays. It provides the necessary reagents and materials to quantify ALP levels in biological samples. The kit includes a buffer solution, substrate solution, and standards for conducting the assay. The core function of the ALP kit is to enable the measurement and analysis of ALP activity in a controlled and standardized manner.
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60 protocols using «alp kit»
In Vitro Osteogenic Evaluation of CeO2 Nanoparticles
Osteogenic Differentiation Assessment
in extraction liquid from scaffolds for 7 days, alkaline phosphatase
(ALP) staining was conducted to assess the osteogenic differentiation.
Rat osteoblasts, initially cultured, were rinsed with PBS, fixed in
4% paraformaldehyde for 25 min, and then rinsed with PBS once more.
Subsequently, the cells underwent staining with a BCIP/NBT ALP kit
(Beyotime, Shanghai, China). Osteocalcin (OCN) or collagen type 1
(COL1) was determined by analyzing the cell culture media with an
OCN or COL1 content assay kit (NanjingjianchengBio Corporation, Nanjing,
China). To measure ALP activity, the cells were lysed, washed, and
resuspended in PBS for the assessment of the optical density (OD)
value at 405 nm using an ALP kit (NanjingjianchengBio Corporation,
Nanjing, China).
Osteoblast ALP Activity under Hypoxia
Quantification of Cellular ALP Activity
Osteogenic Differentiation of PDLSCs
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