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Alp kit

Manufactured by Nanjing Jiancheng
Sourced in China
About the product

The ALP kit is a laboratory equipment product designed for performing alkaline phosphatase (ALP) assays. It provides the necessary reagents and materials to quantify ALP levels in biological samples. The kit includes a buffer solution, substrate solution, and standards for conducting the assay. The core function of the ALP kit is to enable the measurement and analysis of ALP activity in a controlled and standardized manner.

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The spelling variants listed below correspond to different ways the product may be referred to in scientific literature.
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60 protocols using «alp kit»

1

In Vitro Osteogenic Evaluation of CeO2 Nanoparticles

2025
MC3T3-E1 cells were seeded into six-well plates at 10×104 cells per well and divided into five groups: the first group was the blank control group. The second group was treated with P.g-LPS (1 µg/mL) for 4 h to establish an in vitro inflammation model. The third group was pretreated with P.g-LPS for 4 h and then treated with CA-074Me (100 μM) as a positive control group. Four to five groups were treated with P.g-LPS (1 µg/mL) for 4 h followed by the addition of safe concentrations of the groups of hCeO2 and hCeO2@CA-074Me NPs. MC3T3-E1 cells were cultured in a 6-well plate with growth medium. When the cells reached 80% confluence, they were cultured in osteogenic induction medium, which consisted of 10% FBS, 90% DMEM, 0.1 μM dexamethasone, 50 µM ascorbic acid-2-phosphate, and 10 mM β-glycerophosphate. The medium was replaced every three days. After 7, 14, and 21 d of induction, total RNA was extracted and quantitative real time-polymerase chain reaction (RT-PCR) was performed to detect the expression of osteogenesis-related genes. The activity of alkaline phosphatase (ALP) was detected using an ALP kit (Jiancheng, Nanjing, China) and stained with a BCIP/NBT Alkaline Phosphatase Color Development Kit (Beyotime, Shanghai, China). After 21 d of induction, alizarin red S staining was used to evaluate cell mineralization in vitro (Beyotime, Shanghai, China). This process was repeated three times for each group of samples.
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2

Osteogenic Differentiation Assessment

2025
Following incubation
in extraction liquid from scaffolds for 7 days, alkaline phosphatase
(ALP) staining was conducted to assess the osteogenic differentiation.
Rat osteoblasts, initially cultured, were rinsed with PBS, fixed in
4% paraformaldehyde for 25 min, and then rinsed with PBS once more.
Subsequently, the cells underwent staining with a BCIP/NBT ALP kit
(Beyotime, Shanghai, China). Osteocalcin (OCN) or collagen type 1
(COL1) was determined by analyzing the cell culture media with an
OCN or COL1 content assay kit (NanjingjianchengBio Corporation, Nanjing,
China). To measure ALP activity, the cells were lysed, washed, and
resuspended in PBS for the assessment of the optical density (OD)
value at 405 nm using an ALP kit (NanjingjianchengBio Corporation,
Nanjing, China).
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3

Osteoblast ALP Activity under Hypoxia

2024
After subjecting osteoblasts to axial stretch for 3 or 7 days under hypoxia, we used an ALP kit (Nanjing Jiancheng Bioengineering Institute, Jiangsu, China) to detect ALP activity. The osteoblasts were then lysed at 4 °C for 12 h using an equal part (100µL) of cell lysate containing 0.05% Triton X-100. For the ALP assay, 20µL of the sample and 100µL of substrate buffer (containing p-nitrophenyl phosphate) were added to each well of a 96-well culture plate. The plate was shaken for 1 min and incubated at 37 °C for 15 min. After incubation, 80µL of the reaction solution termination solution was added to each well and shaken for another minute. Finally, we determined the absorbance at a wavelength of 520 nm. The nitrophenol level was then calculated according to the instructions provided in the ALP kit. To determine the intracellular protein content, we used a bicinchoninic acid (BCA) protein assay kit (Nanjing Jiancheng Bioengineering Institute, Jiangsu, China) following the manufacturer’s instructions. With reference to the normal standards, we obtained the total protein content. Relative ALP activity was calculated by dividing the amount of nitrophenol by the corresponding total protein, following the instructions provided in the ALP kit.
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4

Quantification of Cellular ALP Activity

2023
The cell samples of each group were resuspended with PBS, and the cells were broken by ultrasonic broken instrument (Ningbo Scientz Biotechnology Co.,Ltd., Ningbo) under the condition of ice bath on differentiation day 7. The protein content of the cell suspension was determined by BCA assay kit (Solarbio, Beijing). For quantification of ALP activity, ALP kit (Nanjing Jiancheng, Nanjing) was used.
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5

Osteogenic Differentiation of PDLSCs

2023
PDLSCs were cultured in a 6-well plate with growth medium. When the cells reached 80% confluence, the cells were cultured in osteogenic induction medium (α-MEM containing 10% FBS, 0.1 μM dexamethasone, 50 μM ascorbate-2-phosphate and 10 mM β-glycerophosphate; Sigma‒Aldrich, St. Louis, USA) and replaced every three days. After 7 days of induction, total RNA was extracted, and quantitative real time-polymerase chain reaction (qRT‒PCR) was performed to detect the expression of osteogenesis-related genes. The activity of alkaline phosphatase (ALP) was detected by an ALP kit (Jiancheng, Nanjing, China) and stained with a BCIP/NBT Alkaline Phosphatase Color Development Kit (Beyotime, Shanghai, China). Then, the sections were scanned, and pictures were taken. After 21 days of induction, alizarin red S staining was used to evaluate cell mineralization in vitro (Solarbio, Beijing, China). We repeated this process three times for each group of samples.
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