Microplate reader
The Microplate reader is a laboratory instrument used to measure the absorbance, fluorescence, or luminescence of samples in a microplate format. It is designed to quantify and analyze a wide range of biological and chemical assays, such as enzyme-linked immunosorbent assays (ELISA), cell-based assays, and other microplate-based applications.
Lab products found in correlation
6 925 protocols using Microplate reader
Oxidative Stress Enzyme Assays
Metabolic Profiling of Cultured Cells
The culture medium was collected for the measurement of lactate release from cells. Lactate levels were determined using a lactate colorimetric assay kit (BioVision) according to the manufacturer’s protocol. The absorbance at 450 nm was recorded using a BioTek microplate reader and normalized to protein concentration.
ATP and ADP levels were measured using an ATP/ADP colorimetric assay kit (BioVision) according to the manufacturer’s protocol. The absorbance at 450 nm was recorded using a BioTek microplate reader and normalized to protein concentration.
Melanin Quantification Assay with Essential Oils
Plasma Biochemical Analysis Protocol
Colorimetric Assay for PTP1B Activity
For in vitro PTP1B assay, different concentrations of drugs were incubated with PTP1B (0.4 μg) and 4 mmol/L p-nitrophenyl phosphate in 50 mmol/L potassium phosphate buffer (pH 7.5), containing 0.1 mmol/L EDTA. Incubations were carried out for 30 min at 37°C and terminated by the addition of NaOH. The p-nitrophenol formed was determined at 400 nm using a BioTek microplate reader.
Proliferation and Cytotoxicity Assay of ATB
To analyze the cytotoxicity of ATB to HCT116/ATB and LOVO/ATB cells, different concentrations of ATB were added to the cell culture. Then, the plate was cultured for 48 hours at 37°C, 5% CO2. Then, 10 μL of CCK8 solution was added to each well, and the cells were further cultured for 1–4 hours. The cytotoxicity of ATB to HCT116/ATB and LOVO/ATB cells was measured using a Microplate reader (BioTek).
Cell viability and proliferation assays
Nitrite, TNF-α, and IL-6 Quantification
Serum and heart tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) were quantified by available ELISA kits (eBioscience Co., San Diego, CA, USA) according to manufacturers’ instruction. The absorbance was measured using a microplate reader (Biotek, USA) and concentration of each factor was calculated by comparison curve established in the same measurement. Each cytokine assay was performed in duplicate each time.
Liver Inflammatory Biomarker Quantification
Cytotoxicity Evaluation of CuO NPs
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