Microplate enzyme immunoassay kit

Manufactured by Monobind

Sourced in United States

The Microplate Enzyme Immunoassay kit is a laboratory instrument designed for conducting immunoassay tests. It is used to detect and measure the presence of specific analytes in a sample by utilizing enzyme-linked immunosorbent assay (ELISA) technology. The kit provides a standardized platform for performing multiple immunoassay tests simultaneously in a 96-well microplate format.

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Enzyme immunoassay kit (2 citations)

5 protocols using microplate enzyme immunoassay kit

Hormones Profiling in Cell Culture

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The levels of hormones including 17-ß estradiol (E2),
progesterone (P4), as well as dehydroepiandrosterone
(DHEA) were measured by a Microplate Enzyme
Immunoassay kit (sensitivity=6.5 pg/mL, Monobind Inc.,
USA), an enzyme-linked immunosorbent (ELISA) assay
kit (sensitivity=0.1 ng/mL, DiaPlus Inc., USA), and a
Microplate Enzyme Immunoassay kit (sensitivity=0.04
mg/mL, Monobind Inc., USA), respectively. On the last
day of culture, half of the culture media was collected.

Majdi Seghinsara A., Shoorei H., Hassanzadeh Taheri M.M., Khaki A., Shokoohi M., Tahmasebi M., Khaki A.A., Eyni H., Ghorbani S., Riahi Rad K., Kalarestaghi H, & Roshangar L. (2019). Panax ginseng Extract Improves Follicular Development after Mouse Preantral Follicle 3D Culture. Cell Journal (Yakhteh), 21(2), 210-219.

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Hormone Quantification in Follicle Culture

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At the end of the culture period, day 12, the levels of 17-β estradiol (E2), progesterone (P4), and DHEA hormones were measured in collected media derived from follicle culture. The levels of E2 were measured by a Microplate Enzyme Immunoassay kit (monobind, sensitivity = 6.5 pg/ml), of P4 by an enzyme-linked immunosorbent assay kit (DIAPLUS, sensitivity = 0.1 ng/ml), and of DHEA by a Microplate Enzyme Immunoassay kit (monobind, sensitivity = 0.04 mg/ml). These experiments were minimally done in triplicates.

Shoorei H., Khaki A., Ainehchi N., Hassanzadeh Taheri M.M., Tahmasebi M., Seyedghiasi G., Ghoreishi Z., Shokoohi M., Khaki A.A, & Abbas Raza S.H. (2018). Effects of Matricaria chamomilla Extract on Growth and Maturation of Isolated Mouse Ovarian Follicles in a Three-dimensional Culture System. Chinese Medical Journal, 131(2), 218-225.

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Quantitative Serum Testosterone ELISA

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The serum testosterone concentrations was determined quantitatively using the Microplate Enzyme Immunoassay kit for total testosterone concentration in human serum as described in the manufacturer’s test procedure (Accu-Blind ELISA Microwells, Product code: 3725–300, Monobind Inc., USA).

Erhabor J.O, & Idu M. (2017). Aphrodisiac potentials of the ethanol extract of Aloe barbadensis Mill. root in male Wistar rats. BMC Complementary and Alternative Medicine, 17, 360.

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Ovarian Tissue Endocrine Function

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To evaluate the endocrine function of ovarian tissue, the concentration of E2 and AMH were measured in the collected media derived from cultured ovarian fragments on day 2 and 14 of culture period. The levels of E2 were measured by a Microplate Enzyme Immunoassay kit (Monobind, USA, sensitivity=6.5 pg/ml) and AMH by an Enzyme-Linked Immunosorbent Assay (Ultra-sensitive AMH/MIS, USA, sensitivity= 4.4±0.9 pg/ml).

Ramezani M., Salehnia M, & Jafarabadi M. (2017). Short Term Culture of Vitrified Human Ovarian Cortical Tissue to Assess the Cryopreservation Outcome: Molecular and Morphological Analysis. Journal of Reproduction & Infertility, 18(1), 162-171.

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Testosterone, LH, and FSH Quantification

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Plasma testosterone TET, luteinizing hormone (LH) and follicle stimulating
hormone (FSH) assays were performed using a commercial microplate enzyme
immunoassay kit, following the manufacturer’s instructions (Monobind Inc., USA).
The testosterone AccuBind™ Microplate EIA Test System has a sensitivity
of 0.0576 ng/ml and with a negligible cross reactivity with other androgen
derivatives like androstenedione, 5α-dihydrotestosterone, and
methyltestosterone.

Ezejiofor A.N, & Orisakwe O.E. (2019). The protective effect of Costus afer Ker Gawl aqueous leaf extract on lead-induced reproductive changes in male albino Wistar rats. JBRA Assisted Reproduction, 23(3), 215-224.

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