The compounds in EHF were analyzed by HPLC/MS, and the specific method of operation was reported previously (Zhang et al., 2017 (link)). An Agilent 1100 HPLC system and DAD and an LC/MSD Trap XCT ESI mass spectrometer (Agilent Technologies, MA, USA) were used for analysis. The separation was performed on a GS-120-5-C18-BIO chromatographic column (5 µm, 250 × 4.6 mm i.d.) with the column temperature set at 35 °C. A linear gradient elution of A (0.1% formic acid water) and B (acetonitrile) was used with the gradient procedure as follows: 0 min, B 5%, to 60 min B 40% (v/v). The flow rate was 1.0 ml/min and the injection volume was 10 µl. DAD was on and the target wavelength was simultaneously set at 210 nm. The split ratio to the mass spectrometer was 1:3. The acquisition parameters for negative ion mode were: collision gas, ultra-high purity helium (He), nebulizer gas (N2), 35 psi, drying gas (N2), 10 l/min, drying temperature, 350°C, HV, 3500 V, mass scan range, m/z 100–2,200, target mass, 500 m/z, compound stability, 100%, trap drive level, 100%. All the data were analyzed by Chemstation software.
Lc msd trap xct esi mass spectrometer
Manufactured by Agilent Technologies
Sourced in United States
The LC/MSD Trap XCT ESI mass spectrometer is a powerful analytical instrument designed for the detection and identification of chemical compounds. It utilizes liquid chromatography (LC) coupled with electrospray ionization (ESI) and an ion trap mass spectrometer (MSD Trap) to provide accurate mass measurements and structural information for a wide range of molecules.
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5 protocols using lc msd trap xct esi mass spectrometer
1
HPLC-MS Analysis of EHF Compounds
2
HPLC-based Quantification of BSHX
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The components of BSHX were measured using an Agilent 1100 HPLC system equipped with a quaternary pump, auto-sampler, degasser, automatic thermostatic column compartment, DAD, and LC/MSD Trap XCT ESI mass spectrometer (Agilent Technologies, MA, USA), as previously described [23 (link)].
3
HPLC-MS Protocol for Compound Analysis
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An Agilent 1100 HPLC system, equipped with a quaternary pump, an autosampler, a degasser, an automatic thermostatic column compartment, a DAD and an LC/MSD Trap XCT ESI mass spectrometer (Agilent Technologies, MA, USA), was used for the separation. The separation was performed on a GS-120-5-C18-BIO chromatographic column (5 µm, 250 ⋅ 4.6 mm i.d.) with the column temperature set at 35°C. A linear gradient elution of A (0.1% formic acid water) and B (acetonitrile) was used with the gradient procedure as follows: 0 min, B 5%, to 60 min B 40% (v/v). The ow rate was 1.0 ml/min and the injection volume was 10 µL. DAD was on and the target wavelength was simultaneously set at 210 nm. The split ratio to the mass spectrometer was 1:3. The acquisition parameters for negative ion mode were: collision gas, ultra high-purity helium (He), nebulizer gas (N2), 35 psi, drying gas (N2), 10 l/min, drying temperature, 350 °C, HV, 3500 V, mass scan range, m/z 100-2200, target mass, 500 m/z, compound stability, 100%, trap drive level, 100%. All the data were analysis by Chemstation software.
4
HPLC-MS Analysis of Herbal Compounds
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An Agilent 1100 HPLC system, equipped with a quaternary pump, an autosampler, a degasser, an automatic thermostatic column compartment , a DAD and an LC/MSD Trap XCT ESI mass spectrometer (Agilent Technologies, MA, USA), was used for the analysis of the herb. The separation was performed on a GS-120-5-C18-BIO chromatographic column (5 m, 250 4.6 mm i.d.) with the column temperature set at 35℃. A linear gradient elution of A (0.1% formic acid water) and B (acetonitrile
was used with the gradient procedure as follows: 0 min, B 5%, to 60 min B 40% (v/v).
The flow rate was 1.0 mL/min and the injection volume was 10 L. DAD was on and the target wavelength was simultaneously set at 210 nm. The split ratio to the mass spectrometer was 1:3. The acquisition parameters for negative ion mode were: collision gas, ultra high-purity helium (He), nebulizer gas (N2), 35 psi, drying gas (N2), 10 L/min, drying temperature, 350°C, HV, 3500 V, mass scan range, m/z 100-2200, target mass, 500 m/z, compound stability, 100%, trap drive level, 100%. All the data were analyzed by Chemstation software.
was used with the gradient procedure as follows: 0 min, B 5%, to 60 min B 40% (v/v).
The flow rate was 1.0 mL/min and the injection volume was 10 L. DAD was on and the target wavelength was simultaneously set at 210 nm. The split ratio to the mass spectrometer was 1:3. The acquisition parameters for negative ion mode were: collision gas, ultra high-purity helium (He), nebulizer gas (N2), 35 psi, drying gas (N2), 10 L/min, drying temperature, 350°C, HV, 3500 V, mass scan range, m/z 100-2200, target mass, 500 m/z, compound stability, 100%, trap drive level, 100%. All the data were analyzed by Chemstation software.
5
HPLC-MS Separation of DBTL Constituents
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An Agilent 1100 HPLC system, equipped with a quaternary pump, an autosampler, a degasser, an automatic thermostatic column compartment, a DAD and an LC/MSD Trap XCT ESI mass spectrometer (Agilent Technologies, MA, USA), was used for the separation of the constituents of DBTL. The separation was performed on a GS-120-5-C18-BIO chromatographic column (5 m, 250 4.6 mm i.d.) with the column temperature set at 35C. A linear gradient elution of A (0.1% formic acid water) and B
(acetonitrile) was used with the gradient procedure as follows: 0 min, B 5%, to 60 min B 40% (v/v). The flow rate was 1.0 mL/min and the injection volume was 10 L. DAD was on and the target wavelength was simultaneously set at 210 nm the split ratio to the mass spectrometer was 1:3. The acquisition parameters for negative ion mode were: collision gas, ultra high-purity helium (He), nebulizer gas (N2), 35 psi, drying gas (N2), 10 L/min, drying temperature, 350°C, HV, 3500 V, mass scan range, m/z 100-2200, target mass, 500 m/z, compound stability, 100%, trap drive level, 100%. All the data were analysed by Chemstation software.
(acetonitrile) was used with the gradient procedure as follows: 0 min, B 5%, to 60 min B 40% (v/v). The flow rate was 1.0 mL/min and the injection volume was 10 L. DAD was on and the target wavelength was simultaneously set at 210 nm the split ratio to the mass spectrometer was 1:3. The acquisition parameters for negative ion mode were: collision gas, ultra high-purity helium (He), nebulizer gas (N2), 35 psi, drying gas (N2), 10 L/min, drying temperature, 350°C, HV, 3500 V, mass scan range, m/z 100-2200, target mass, 500 m/z, compound stability, 100%, trap drive level, 100%. All the data were analysed by Chemstation software.
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