Formvar-Insulated Nichrome Wire (bare diameter: 17.78 μm, A-M systems, WA, USA) was twisted in groups of four as tetrodes (impedance: 0.5–0.8 MΩ at 1 kHz). Two 20 cm-long wires were folded in half over a horizontal bar for twisting. The ends were clamped together and manually twisted clockwise. Finally, their insulation coating was fused with a heat gun at the desired level of twist and cut in the middle to produce two tetrodes. To reinforce each tetrode longitudinally, each tetrode was then inserted into Polymide tubing (inner diameter: 0.045 inches; wall: 0.005 inches; A-M systems, WA, USA) and fixed in place by cyanoacrylate glue. An array of 2 × 4 tetrodes were then assembled using an inter-tetrode gap of 0.4–0.5 mm. After assembly, the insulation coating of each wire was gently removed at the tip, and then the wire was soldered to a connector pin. The reference electrode used was a tip-exposed Ni-Chrome wire of diameter 50.8 μm (A-M systems, WA, USA), and a ground electrode was a piece of copper wire of the diameter of 0.1 mm. Both of these were also soldered to a connector pin. The tetrodes and reference were then carefully cemented by silicon gel and trimmed to an appropriate length immediately before implantation.
Polymide tubing
Manufactured by A-M Systems
Sourced in United States
Polyimide tubing is a flexible, high-temperature resistant material used in a variety of laboratory and medical applications. It has excellent chemical and thermal stability, making it suitable for use in demanding environments.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using polymide tubing
1
Tetrode Array Construction for Neural Recording
2
Tetrode Array Fabrication Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Formvar-Insulated Nichrome Wire (bare diameter: 17.78 μm, A-M systems, WA, USA) was twisted in groups of four as tetrodes (impedance: 0.5-0.8 MΩ at 1 kHz). Two 20 cm-long wires were folded in half over a horizontal bar for twisting. The ends were clamped together and manually twisted clockwise. Finally, their insulation coating was fused with a heat gun at the desired level of twist and cut in the middle to produce two tetrodes. To reinforce each tetrode longitudinally, each tetrode was then inserted into Polymide tubing (inner diameter: 0.045 inches; wall: 0.005 inches; A-M systems, WA, USA) and xed in place by cyanoacrylate glue. An array of 2×4 tetrodes were then assembled using an inter-tetrode gap of 0.4-0.5 mm. After assembly, the insulation coating of each wire was gently removed at the tip, and then the wire was soldered to a connector pin. The reference electrode used was a tip-exposed Ni-Chrome wire of diameter 50.8μm (A-M systems, WA, USA), and a ground electrode was a piece of copper wire of the diameter of 0.1mm. Both of these were also soldered to a connector pin. The tetrodes and reference were then carefully cemented by silicon gel and trimmed to an appropriate length immediately before implantation.
3
Fabrication of Tetrode Microelectrode Array
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Formvar-Insulated Nichrome Wire (bare diameter: 17.78 μm, A-M systems, WA, USA) was twisted in groups of four as tetrodes (impedance: 0.5-0.8 MΩ at 1 kHz). Two 20 cm-long wires were folded in half over a horizontal bar for twisting. The ends were clamped together and manually twisted clockwise.
Finally, their insulation coating was fused with a heat gun at the desired level of twist and cut in the middle to produce two tetrodes. To reinforce each tetrode longitudinally, each tetrode was then inserted into Polymide tubing (inner diameter: 0.045 inches; wall: 0.005 inches; A-M systems, WA, USA) and fixed in place by cyanoacrylate glue. An array of 2×4 tetrodes were then assembled using an intertetrode gap of 0.4-0.5 mm. After assembly, the insulation coating of each wire was gently removed at the tip, and then the wire was soldered to a connector pin. The reference electrode used was a tipexposed Ni-Chrome wire of diameter 50.8μm (A-M systems, WA, USA), and a ground electrode was a piece of copper wire of the diameter of 0.1mm. Both of these were also soldered to a connector pin.
The tetrodes and reference were then carefully cemented by silicon gel and trimmed to an appropriate length immediately before implantation.
Finally, their insulation coating was fused with a heat gun at the desired level of twist and cut in the middle to produce two tetrodes. To reinforce each tetrode longitudinally, each tetrode was then inserted into Polymide tubing (inner diameter: 0.045 inches; wall: 0.005 inches; A-M systems, WA, USA) and fixed in place by cyanoacrylate glue. An array of 2×4 tetrodes were then assembled using an intertetrode gap of 0.4-0.5 mm. After assembly, the insulation coating of each wire was gently removed at the tip, and then the wire was soldered to a connector pin. The reference electrode used was a tipexposed Ni-Chrome wire of diameter 50.8μm (A-M systems, WA, USA), and a ground electrode was a piece of copper wire of the diameter of 0.1mm. Both of these were also soldered to a connector pin.
The tetrodes and reference were then carefully cemented by silicon gel and trimmed to an appropriate length immediately before implantation.
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