Human insulin elisa kit
The Human Insulin ELISA Kit is a quantitative enzyme-linked immunosorbent assay (ELISA) for the measurement of human insulin levels in biological samples. The kit utilizes a monoclonal antibody specific for human insulin that is coated on a microplate. Samples and standards are pipetted into the wells and any insulin present is bound by the immobilized antibody. After washing, an enzyme-linked polyclonal antibody specific for insulin is added and binds to the captured insulin. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added and color develops in proportion to the amount of insulin bound. The intensity of the color is measured using a microplate reader.
Lab products found in correlation
31 protocols using human insulin elisa kit
Glucose-Stimulated Insulin Secretion Assay
FAHFA Biosynthesis and Bioassays
Quantitative Platelet and Pancreatic Assays
Insulin Secretion in INS-1E Cells
Fasting Blood Lipid and Glucose Profile
Insulin Secretion Assessment in IPCs
Intranasal Human Insulin Pharmacokinetics
Plasma Metabolite Analysis by LC-MS
Glucose-Stimulated Insulin Secretion Assay
Biochemical and Hormone Profile Analysis
Immunoassay was performed via chemiluminescent detection for vitamin B12, total 25(OH)D3, insulin, sex hormone-binding globulin (SHBG), and dehydroepiandrosterone sulfate (DHEA-S). Insulin levels were assessed using a Human Insulin ELISA kit (ALPCO Diagnostics) with inter- and intra-assay precision below 15%.
The enzymatic method was used to assess blood glucose levels using an Abbot ALinity I analyzer. Ca, P, and ALP concentrations were estimated using Abbot ALinity C Analyzer (Abbott, Abbott Park, Chicago, IL, USA). Androstenedione was analyzed using an Immulite Siemens analyzer (Siemens Healthcare GmbH, Erlangen, Germany).
HbA1c (%) was assessed with a Siemens DCA Vantage analyzer (Siemens Healthcare GmbH, Erlangen, Germany).
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