Rat insulin elisa kit

Fasting blood glucose and serum insulin were estimated using the glucometer method (Accutrend alpha, Roche Diagnostic GMbH, Germany) and rat insulin ELISA kit (Wuhan Fine Biotech Co., Ltd., China), respectively. Systemic insulin resistance was estimated using the homeostasis model assessment index for insulin resistance (HOMA-IR) using the following equation: $HOMA-IRindex=\left[fastingglucose\left(mg/dl\right)\times fastinginsulin\left(uU/ml\right)\right]/405)$ . The triglyceride and glucose (TyG) index, is an important predictor for fatty liver and hepatic insulin resistance. This index was estimated using the following formula $TyGindex=\mathit{Ln}\left[triglycerides\left(mg/dl\right)\times fastingglucose\left(mg/dl\right)/2\right]$ . To determine insulin sensitivity, the quantitative insulin sensitivity check index $\left(QUICKI\right)=1/\left[\mathit{log}fastinginsulin\left(uU/ml\right)+\mathit{log}fastingglucose\left(mg/dl\right)\right]$ was used. To determine the secretory capacity of the pancreas, HOMA-B index was calculated as follows: $HOMA-Bindex=20\times fastinginsulin\left(uU/ml\right)/\left[fastingglucose\left(mg/dl\right)-63\right]$ (Abo-elmatty et al., 2013 (link); Yoon et al., 2016 (link); Zhang et al., 2017 (link)).

Serum insulin was measured using a rat insulin ELISA Kit (Wuhan Fine Biotech Co., Ltd. Wuhan, China) according to the manufacturer’s instructions. The results obtained are expressed as µIU/mL. Further, HOMA-IR was used to identify insulin resistance. HOMA-IR was calculated using the following formula [105 (link),106 (link)].