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Hmgb1 elisa kit

Manufactured by Aviva Systems Biology
Sourced in United States

The HMGB1 ELISA Kit is a laboratory product designed to quantify the levels of HMGB1 (High Mobility Group Box 1) protein in biological samples. It is a sandwich enzyme-linked immunosorbent assay (ELISA) that uses specific antibodies to detect and measure the concentration of HMGB1 in various sample types.

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4 protocols using hmgb1 elisa kit

1

Quantifying Extracellular ATP and HMGB1

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1 × 105 of HCT116 VPS4B−/− cells were seeded per well of 12‐well plates and either left non‐transfected or forward‐transfected with siRNAs (control or targeting VPS4A). After 16‐h growth, media were removed and cells were cultured in 0.6 ml of fresh medium for further 52–56 h. Next, media were collected and centrifuged at 300 × g for 10 min, followed by centrifugation at 2,000 × g for 10 min. Media stored at −80°C were used for the following measurements: ATP was quantified using ENLITEN® ATP Assay System (Promega, FF2000) and HMGB1 was quantified using HMGB1 ELISA Kit (Aviva Systems Biology, OKCD04074). All procedures were performed according to the manufacturers’ instructions.
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2

HMGB1 Protein Quantification

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Concentration of the HMGB1 protein in the cell culture medium was measured according to the manufacturer’s instructions using the HMGB1 ELISA kit (Aviva Systems Biology, OKEH00424).
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3

HMGB1 Protein Concentration Quantification

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Concentration of the HMGB1 protein in the cells growing medium was measured according to the manufacturer’s instructions using the HMGB1 ELISA kit (OKEH00423, Aviva systems biology, USA).
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4

Synthesis and Characterization of Cy5.5-Labeled Immune Checkpoint Inhibitor

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2-Chlorotrityl chloride resin (1.2 mmol/g) was purchased from Nankai University resin Co., Ltd. Fmoc-amino acids were obtained from GL Biochem (Shanghai, China). Cy5.5 NHS ester (non-sulfonated) purchased from APEXBIO. Alkali phosphatase (30 U/μL) was obtained from Takara (D2250, Dalian, China) Bio. Inc. Enhanced ATP Assay Kit (S0027) purchased from Beyotime. Both of anti-HMGB1 antibody (ab79823) and anti-carlreticulin antibody (ab92516) were purchased from Abcam. Calreticulin Rabbit mAb (Alexa Fluor 488 conjugate, #62304) was obtained from Cell Signaling Technology. HMGB1 ELISA Kit (OKEH00424) was purchased from AVIVA systems biology. InvivoMAb antimouse PD-L1 (B7-H1) was obtained from BioXcell. APC anti-mouse CD3 antibody (100235), FITC anti-mouse CD8a antibody (100705), APC anti-mouse IFN-γ (505810), PE anti-mouse CD80 (104707), FITC anti-mouse CD86 (105005), Alexa Fluor 647 anti-mouse CD11c (117314), ELISA MAX™ Deluxe Mouse IFN-γ, ELISA MAX™ Deluxe Mouse TNF-α and ELISA MAX™ Deluxe Mouse IL-6 were purchased from Biolegend. Collagenase IV and DNase I were both purchased from Solarbio. Commercially available reagents and solvents were used without further purification, unless noted otherwise.
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