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Hi9146

Manufactured by Hanna Instruments
Sourced in United States, Italy, United Kingdom

The HI9146 is a portable pH and temperature meter designed for laboratory and field use. It features a high-accuracy pH measurement range of 0.00 to 14.00 pH with a resolution of 0.01 pH. The meter also measures temperature from -20.0 to 120.0°C with a resolution of 0.1°C. The HI9146 is equipped with automatic temperature compensation and a large, easy-to-read LCD display.

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11 protocols using hi9146

1

Characterization of Landfill Leachate

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Physicochemical properties of leachate sample such pH, total dissolved solids (TDS), dissolved oxygen (DO) were measured by portable meters pocket type HI9146 (HANNA), HI98301 (HANNA) and HI9146 (HANNA), respectively. To determine the COD values of the leachate samples Colorimeter (HACH, DR/890), COD Reactor (HACH, DRB 200) and COD Digestion Reagent High Range Vial (HACH) were used. The dry weight (DW) content of leachate samples was determined by taking a portion of the sample into the preweighed porcelain crucibles cleaned and dried in an oven previously and oven-dried at 105 °C for 24 h. Then the oven-dried sample was heated in the muffle furnace at 550 °C for 2 hour. The volatile dry weight (VDW) content was measured by calculating the differences between the two weights. The VDW corresponds to the organic fraction of samples (Simon et al., 2009 (link)). TOC of liquid samples were determined by Total Organic Carbon (TOC) Analyzer using combustion technique in the presence of oxidative catalyst working at 680 °C with non-dispersive infrared detection NDIR system (TOC 5000A, Shimadzu).
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2

Fenton Catalysis of Melanin-like Nanomaterial

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To demonstrate the Fenton catalytic effect of MFN, 2.5 mM of H2O2 and 2.5 mM of MFN were mixed in phosphate-buffered saline (PBS). Then, 150 µL of the solution was added to 300 µL of a Ti(SO4)2 solution (1.33 mL of 24% Ti(SO4)2 + 8.33 mL of H2SO4 in 50 mL of deionized water) every 10 min. The concentration of H2O2 was evaluated by measuring the absorbance at 408 nm using a UV-2600 spectrophotometer (Shimadzu). To investigate the capability of MFN to produce oxygen, dissolved oxygen was removed by bubbling the solution with argon. Subsequently, 0.5 mM of H2O2 was incubated with 0.5 mM of MFN in PBS. The O2 concentration was monitored using a dissolved oxygen meter (HI9146, HANNA Instruments, Woonsocket, RI, USA) every 5 min.
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3

Dissolved Oxygen Levels for Marine Fish

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The dissolved oxygen (DO) level of the spent solution after herbal colouration was measured with a dissolved oxygen meter Hanna, HI 9146 microprocessor. Table 4 shows the DO level requirements of marine fish in mg/l (1 mg/l = 1 ppm). The DO level could be also be expressed in ppm. 

Dissolved oxygen level requirement of marine fish (Horne and Goldman 1994 )

Fish survivalDissolved oxygen (mg/ l) level
All fish die0–4
Very few fish live4–6.5

Big fish live

Small fish die

6.5–9.5
All fish live9.5–12
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4

Physicochemical Parameters Influencing Macrobrachium

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Measurements of water physicochemical parameters of the rivers were done according to APHA (1998) and Rodier, Legube, and Brunet (2009) standards to see whether they have an influence in the distribution of Macrobrachium species in the three regions. Water temperature and dissolved oxygen were monitored monthly using oxygen meter (HI 9146, Hanna, Italy), while pH was measured using a pH meter (HI 98129, Hanna, USA).
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5

Water Quality Maintenance for Trout Fry

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To maintain the water pH of these tanks, sodium hydroxide (NaOH) was added to raise the pH, and Hydrochloric acid (HCl) was added to reduce pH if required. The total water hardness was maintained by adding the Calcium sulfate (CaSO4) salts to increase its hardness and magnesium sulfate (MgSO4) to decrease the hardness. The scheduled photoperiod during the experimental period was 12L: 12D. The water quality parameters were determined at twelve hours intervals during the experiment. Total dissolved oxygen (mg/L−1) was measured using a portable digital DO-meter (Model: HI 9146, Hanna, UK), the water temperature was determined with a Celsius glass thermometer, pH with a digital pen pH meter (Orion, model 201, USA), and salinity with a handheld refractometer (Hanna, UK). Total hardness (mg L−1), electric conductivity (mScm1) and total ammonia (mg L−1) were measured with a ProPlus multiparameter (YSI, USA). After acclimation, 25 healthy O. mykiss fry of the same size was randomly stocked in each experimental tank.
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6

Measuring O2 Generation from Zinc-Magnesium Ferrite

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The generation of O2 was measured according to a method reported in the previous literature22 (link) with minor modifications. In summary, 4 mg of Zn1–xMgxFe2O4 was incubated with 20 mL of PBS (pH = 5.8) containing 0.5 mM H2O2 at 37 °C. The dissolved O2 concentration was monitored with an oxygen meter (HI9146, HANNA instruments, Korea) in real time at 60, 120, 180, 240, 300, 360, and 420 min.
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7

Rapid Biodegradability Assessment of Samples

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Rapid biodegradability assays were carried out in glass reactors (0.250 dm -3 ) with 0.095 dm -3 of endogenous activated sludge and 0.005 dm -3 of target sample. Before the assay, the activated sludge was maintained in starvation and continuous aeration overnight to eliminate any residual organic matter and to avoid oxygen limitation. The dissolved oxygen concentration was monitored by using an oximeter (HI9146, Hanna Instruments) as function of the experimental time. The rapid biodegradability of samples was calculated as a relation between rapid biochemical oxygen demand (BOD) and chemical oxygen demand (COD) by using Equation ( 1).
(1)
The rapid BOD is calculated as the difference, in terms of dissolved oxygen decay (mg O2 dm -3 ), between the slope of endogenous activated sludge and the slope when sample is added into endogenous activated sludge.
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8

Tilapia Personality Screening Protocol

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The experiments were conducted in the larviculture laboratory Laqua (Laboratório de Aquacultura) of Universidade Federal de Minas Gerais, Brazil, under protocol number 373/2013 of Comitê de Ética em Experimentação Animal. A total of 324 O. niloticus (40.1 ± 5.3 g, 13.5 ± 1.1 cm) were used for personality classification. The animals were placed in 16 tanks (200 L), with a stocking density of 5.41 g/L, in a recirculating aquaculture system with controlled temperature (28ºC) and pH (7.2 -7.8) (model HI9146 Hanna instruments) and ammonia (<0.004 ppm) (Labcon kit), constant aeration (dissolved oxygen > 5 mg/L) (YSI 6920VZ2 multiparameter probe), 12h/12h photoperiod (digital timer, group Key West DNI), and feed (36% crude protein, 2.6 mm diameter, Laguna/Socil, São Paulo, Brazil) offered until apparent satiety twice a day (8:00am and 4:00pm). The animals were kept in this system for two weeks, with fasting for the last two days prior to personality screening.
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9

Comparative Evaluation of Soybean Meal-Based Diets on Carp Species

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All trials were carried out in triplicates to minimize chances of errors. The fingerlings of Cirhinus mrigala, Labeo rohita and Catla catla were reared into raceways with the dimension ‫׳05×׳22‬ (W×L) for a period of one year in a proportion of 34:33:33 fish/raceway correspondingly (Wahab et al., 2002) (link). Three different diets (SBM I, SBM II and SBM III) were formulated by 80%, 50% and 20% replacement of fish meal with soybean meal from a 45% fish meal diet (control) which constituted 63% of total crude protein. Fish meal and soybean meal, with some other ingredients (Table 1) were pulverized and emulsified to make dough. Pellets were made by a pellet maker machine. Formulated pellets were sun dried for 24 hours before freezing. By following Dada et al. (2002) fish were fed at the rate of 3% body weight.
Dissolved oxygen meter (HI-9146), the microprocessor pH meter (HANNA-HI-8520) and TDS meter (HANNA-HI-98302) were used respectively to determine dissolved oxygen, temperature, pH and total dissolved solids. The temperature of water throughout the experimental trial ranged from 10.1 °C to 30.5 °C, pH ranged 7.8-8.4, while dissolved oxygen was observed within the range of 5.1-8.4 mg/l.
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10

Aquatic Species Creation Protocol

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The parameters of water quality were maintained within the recommended standards for the creation of species, as recommended by Gomes Simões and Araújo-Lima (2010) and Mendonça et al. (2012) . The maximum and minimum water temperatures remained around 27.05 ±0.72 °C (8 h), and 24.91 ±0.72 °C (16 h), respectively, measured daily using a mercury bulb thermometer graduated between 0 and 50 °C. The concentration of dissolved oxygen in the water was around 9.02 ±1.21 ppm, pH 6.19 ±0.95, and total ammonia < 1.00 ppm, measured every three days using a digital pH meter (HI 8424, Hanna ® ), oximeter (HI 9146, Hanna ® ), and commercial colorimetric kit (Arcor ® ) for testing toxic ammonia, respectively.
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