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Anti-GAPDH antibody

Manufactured by Abclone

The Anti-GAPDH antibody is a primary antibody that specifically recognizes the GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) protein. GAPDH is a widely used loading control and housekeeping protein in various biological applications. This antibody can be used to detect and quantify GAPDH levels in various samples, such as cell lysates or tissue extracts, through techniques like Western blotting or immunohistochemistry.

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4 protocols using Anti-GAPDH antibody

Skullcapflavone II (5-hydroxy-2-[2-hydroxy-6-methoxyphenyl]-6,7,8-trimethoxychromen-4-one) was purchased from ChemFaces (Wuhan, Hubei, China). Antibodies against phospho-ERK, ERK2, p38, and NF-κB p65 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against phospho-NF-κB p65 (Ser536), phospho-JNK, JNK, phospho-p38, phospho-c-Jun, and c-Jun were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-phospho-tyrosine antibody (clone 4G10) was purchased from Millipore (Billerica, MA, USA). Anti-collagen type I cleavage-site antibody was purchased from ImmunoGlobe (Himmelstadt, Germany). Anti-GAPDH antibody was purchased from AbClone (Seoul, Korea). Horseradish peroxidase-conjugated goat anti-mouse IgG and rabbit IgG were obtained from KOMA Biotech (Seoul, Korea). Anti-MMP-1 and pro-collagen α1(I) N-propeptide (pN-ColIα1) antibodies were a gift from Dr. Chung, J. H. (Seoul National University College of Medicine, Republic of Korea) [53 (link)]. Alexa Fluor® 488 goat anti-rabbit IgG (H+L) and rhodamine-conjugated phalloidin were purchased from Thermo Fisher Scientific (Waltham, MA, USA).
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Quenched fluorogenic DQ Gelatin and rhodamine-conjugated phalloidin were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Fluorescein isothiocyanate (FITC)-conjugated gelatin, anti-FLAG antibody, and 4′,6-diamidino-2-phenylindole (DAPI) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Genistein, PP1, PP2, and LY294002 were purchased from AG Scientific (San Diego, CA, USA). TKI-258 was purchased from ApexBio (Houston, TX, USA). PD98059 and SP600125 were purchased from Tocris Bioscience (Bristol, UK). QNZ and antibodies against phospho-ERK, ERK2, c-Fos, lamin A/C, phospho-IKKα (Thr23), IKKα, NF-κB p65, CAS, FAK, Src, and HA tag were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against phospho-JNK, JNK, c-Jun, phospho-Src family (Tyr416), phospho-Akt (Ser473), Akt, phospho-IKKα/β (Ser176/180), IKKβ, phospho-IκBα (Ser32), IκBα, phospho-NF-κB p65 (Ser536), and MMP-9 were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-phosphotyrosine antibody (clone 4G10) and anti–MMP-2 antibody were purchased from Millipore (Billerica, MA, USA). Anti-GAPDH antibody was purchased from AbClone (Seoul, Korea). Horseradish peroxidase-conjugated goat anti-mouse IgG, rabbit IgG, and rabbit anti-goat IgG were purchased from KOMA Biotech (Seoul, Korea). Anti-PTK7 antibody was described previously [39 (link)].
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Recombinant human TGF-β1 was purchased from PeproTech (Princeton, NJ, USA). SB431542 was purchased from Selleckchem (Houston, TX, USA). RGD peptide was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). TGF-β neutralizing antibody was purchased from R&D Systems (Minneapolis, MN, USA). Anti-TNC antibodies for IHC staining and western blot analysis were purchased from Santa Cruz Biotechnology and Abcam (Cambridge, UK), respectively. Anti-GAPDH antibody was from AbClone (Seoul, Korea). Anti-SMAD2 (SMAD family member 2), anti-phospho-SMAD2, anti-SMAD3, and anti-phospho-SMAD3 antibodies were from Cell Signaling Technology (Danvers, MA, USA). Anti-pro-COL1A1 and anti-MMP-1 antibodies have been described previously [49 (link)]. Horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG, goat anti-rabbit IgG, and rabbit anti-goat IgG antibodies were purchased from KOMA Biotech (Seoul, Korea). Rhodamine Red-X-conjugated goat anti-mouse IgG and Alexa Fluor 488-conjugated rabbit anti-goat IgG antibodies were purchased from Invitrogen (Carlsbad, CA, USA).
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Recombinant human TGF-β1 and SB431542 were purchased from PeproTech (Princeton, NJ, USA) and Selleckchem (Houston, TX, USA), respectively. Anti-pro-COL1A1 and anti-MMP-1 antibodies were used as described previously [45 (link)]. The anti-GAPDH antibody was purchased from AbClone (Seoul, Republic of Korea). Anti-SMAD2 (SMAD family member 2), anti-phospho-SMAD2, anti-SMAD3, and anti-phospho-SMAD3 antibodies were from Cell Signaling Technology (Danvers, MA, USA). Horseradish peroxidase (HRP)-conjugated goat anti-mouse and anti-rabbit IgG antibodies were purchased from KOMA Biotech (Seoul, Republic of Korea). Rhodamine Red X-conjugated goat anti-mouse IgG (H + L) antibody was purchased from Thermo Fisher Scientific (Waltham, MA, USA).
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