Upon fasting for 12 hrs, the blood samples were collected from the tail vein after anesthesia, and centrifuged at 3,000 rpm for 8 min. Fasting blood glucose (FBG) was measured using a blood glucose meter (One Touch Ultra, Johnson & Johnson, CA, USA). Then serum insulin was detected with EZRMI-13K kit (One Touch Ultra, Johnson & Johnson, CA, USA). Finally, the Homeostasis Model Assessment of Insulin Resistance (HOMA-IR) was calculated to assess the degree of insulin resistance, using the following formula: HOMA-IR=fasting serum insulin (mIU/L)×FBG (mmol/L)/22.5 (22 (link)).
Onetouch ultra
Manufactured by Johnson & Johnson
Sourced in United States, Japan, Brazil, Argentina, China
The OneTouch Ultra is a blood glucose monitoring system designed to help individuals with diabetes track their blood sugar levels. It is a compact, handheld device that uses test strips to measure the glucose concentration in a small blood sample. The device provides fast and accurate readings to assist in the management of diabetes.
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Lab products found in correlation
Dca2000 hba1c reagent kit, by Siemens (3 mentions)
Neutral buffered formalin, by Fujifilm (3 mentions)
Streptozotocin (stz), by Merck Group (3 mentions)
Humulin r, by Eli Lilly (2 mentions)
Streptozotocin (stz), by Johnson & Johnson (2 mentions)
2 deoxy d glucose 2 dg, by Merck Group (2 mentions)
Integra 800, by Roche (1 mentions)
Retrospective cgm system, by Medtronic (1 mentions)
Lactate pro2 lt 1730, by Arkray (1 mentions)
Alloxan, by Merck Group (1 mentions)
Prism software, by GraphPad (1 mentions)
Humulin human insulin, by Eli Lilly (1 mentions)
Enzyme immunoassay kit, by R&D Systems (1 mentions)
Prism version, by GraphPad (1 mentions)
Ultra sensitive mouse insulin elisa, by Crystal Chem (1 mentions)
D glucose g8270, by Merck Group (1 mentions)
Rat insulin elisa, by Mercodia (1 mentions)
Au480, by Beckman Coulter (1 mentions)
Streptozotocin (stz), by Fujifilm (1 mentions)
Phentolamine, by Merck Group (1 mentions)
75 protocols using onetouch ultra
1
Blood Glucose and Insulin Resistance Evaluation
2
Glucose and Pyruvate Tolerance Tests
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Mice were fasted for 8 h, and then, 1 mg/g body weight of glucose or pyruvate was dissolved in sterile sodium chloride. Fasting blood glucose was drawn just before glucose administration, either by oral gavage or intraperitoneal injection using a glucometer (OneTouch Ultra, Lifescan, Johnson & Johnson). Glucose levels were checked over 2 h. Pyruvate was administered through intraperitoneal injection.
3
Glucose and Insulin Tolerance Test
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Mice were fasted for 16 h and 2 h before intraperitoneal (i.p.) injections of 1 g/kg glucose and 0.5 units/kg insulin, respectively. Blood samples were collected from the tail veins at indicated time points after glucose or insulin administration, and blood glucose levels were measured using a blood glucose monitoring system (OneTouch Ultra; Johnson & Johnson).
4
Lipid and Glucose Metabolism Assay
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Serum total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C) and glucose concentrations were measured by enzymatic assay using an automatic biochemical analyzer (ROCHE COBAS INTEGRA 800, Basel, Switzerland) from the fasting mice at the time-points dictated.
Three days before euthanasia, intraperitoneal Glucose Tolerance Test (ipGTT) was performed on 6 hrs fasted mice. The mice (n = 6 each group) were intraperitoneally injected with glucose (2 g/kg bodyweight, 20% glucose solution). Blood samples were respectively obtained from tail vein, at 0, 10, 30, 60, 90 and 120 min. after the glucose load, and blood glucose concentration was measured using a One Touch Ultra glucometer (Johnson&Johnson New Jersey, USA). The area under the curve (AUC) was then determined using the linear method of the trapezoid rule 21 (link),22 (link).
Three days before euthanasia, intraperitoneal Glucose Tolerance Test (ipGTT) was performed on 6 hrs fasted mice. The mice (n = 6 each group) were intraperitoneally injected with glucose (2 g/kg bodyweight, 20% glucose solution). Blood samples were respectively obtained from tail vein, at 0, 10, 30, 60, 90 and 120 min. after the glucose load, and blood glucose concentration was measured using a One Touch Ultra glucometer (Johnson&Johnson New Jersey, USA). The area under the curve (AUC) was then determined using the linear method of the trapezoid rule 21 (link),22 (link).
5
Perioperative Glycemic Monitoring with CGM
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During the perioperative period, a retrospective CGM system (Medtronic Inc.,
Northridge, CA, USA) was used to monitor glycemia for five consecutive days,
including the day of surgery, and 2 days before and after operation. At least
four capillary blood glucose measurements per day were obtained with a
glucometer (OneTouch® Ultra®, Johnson & Johnson, New
Brunswick, NJ, USA) to calibrate each CGM trace. After the 5-day monitoring
period, we calculated the TIR, the time above target range (TAR), the time below
target range (TBR), and the mean amplitude of glycemic excursion (MAGE) to
assess glycemic control. TIR, glucose 3.9–10.0 mmol/L, was computed by
calculating the percentage of time spent in target range during a 24 h period.
TAR (>10.0 mmol/L) and TBR (<3.9 mmol/L) were calculated in a similar
manner; MAGE was determined by calculating the arithmetic mean of the
differences between peaks and nadirs, and only the amplitudes of more than one
standard deviation (SD) of the mean glucose were considered.
Northridge, CA, USA) was used to monitor glycemia for five consecutive days,
including the day of surgery, and 2 days before and after operation. At least
four capillary blood glucose measurements per day were obtained with a
glucometer (OneTouch® Ultra®, Johnson & Johnson, New
Brunswick, NJ, USA) to calibrate each CGM trace. After the 5-day monitoring
period, we calculated the TIR, the time above target range (TAR), the time below
target range (TBR), and the mean amplitude of glycemic excursion (MAGE) to
assess glycemic control. TIR, glucose 3.9–10.0 mmol/L, was computed by
calculating the percentage of time spent in target range during a 24 h period.
TAR (>10.0 mmol/L) and TBR (<3.9 mmol/L) were calculated in a similar
manner; MAGE was determined by calculating the arithmetic mean of the
differences between peaks and nadirs, and only the amplitudes of more than one
standard deviation (SD) of the mean glucose were considered.
6
Rodent Exhaustion Testing: Lactic Acid and Glucose
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Blood was collected from the caudal vein of each rat immediately before and after exhaustion testing. Blood lactic acid levels were measured using a simplified blood lactic acid measuring device (LactatePro™ 2 LT‐1730; Arkray, Kyoto, Japan), and blood glucose levels were measured using a glucose self‐measuring device (One Touch UltraⓇ; Johnson & Johnson, Tokyo, Japan).
7
Alloxan-Induced Diabetic Rat Model
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Alloxan (ALX, 40 mg/kg, Sigma®) was dissolved in saline and injected into jugular vein in rats previously fasted for 12 h. Five days after ALX injection, animals with postprandial glycaemia over 250 mg/kg were considered diabetics and included in the experimental protocol [7 (link)]. Blood samples were collected from the tail tip and glycaemia was measured by One Touch Ultra (Johnson & Johnson®) utilizing the glucose oxidase method. Nondiabetic control animals received saline injection as control.
8
Glucose Tolerance in Mice: Diet and Genotype
9
Biomarker Assessment in Experiment
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Random blood glucose testing was conducted using a glucometer (OneTouch Ultra, Johnson & Johnson Co., CA, USA) every other week during the experiment. Blood samples were collected before sacrifice. Hemoglobin A1c (HbA1c) levels were measured using DCA2000 HbA1c reagent kits (Siemens Healthcare Diagnostics, Inc., Tarrytown, NY, USA). Blood samples were centrifuged at 900 × g for 15 min at 4 °C to collect plasma. Serum creatinine and free fatty acid (FFA) levels were measured using ELISA kits obtained from Arbor Assays (Ann Arbor, MI, USA) and BioAssay Systems (Hayward, CA, USA), respectively.
10
Measuring Glycemic and Renal Biomarkers
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Blood glucose and HbA1c levels were measured with a glucometer (OneTouch Ultra; Johnson & Johnson, Milpitas, CA, USA) and with a DCA2000 HbA1c Reagent Kit (Siemens Healthcare Diagnostics, Tarrytown, NY, USA), respectively. Urinary albumin was measured using a competitive enzyme-linked immunosorbent assay (ALPCO, Westlake, OH, USA). Urine spectra were used to determine the urine creatinine concentration, and urine albumin levels were corrected for urine creatinine and presented as the UACR.
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