The largest database of trusted experimental protocols

15 protocols using GLUCOCARD G+ meter

The serum levels of the glucose, lipids, and hormones were measured using appropriate equipment, reagents, and kits. The GLUCOCARD G+ meter was used to measure the glucose content (Arkray, Kyoto, Japan). The NEFA C-Test (Wako Pure Chemical Industries, Osaka, Japan), the Triglyceride E-Test (Wako Pure Chemical Industries), and the Cholesterol E-Test (Wako Pure Chemical Industries) were used for the free fatty acid levels, triglyceride levels, and cholesterol content, respectively. The LBIS Mouse Insulin ELISA kit U-type (Shibayagi, Gunma, Japan) and the Total Thyroxine ELISA kit (ALPCO, Salem, NH, USA) were used to measure the insulin and T4 levels, respectively.
+ Open protocol
+ Expand
Blood glucose levels were measured using a Glucocard G + Meter (Arkray, Kyoto, Japan). Plasma insulin concentrations were determined using a mouse insulin enzyme‐linked immunosorbent assay kit (Fujifilm Wako Pure Chemical Co., Osaka, Japan). Plasma glucagon concentrations were determined using a Mercodia Glucagon enzyme‐linked immunosorbent assay kit (Mercodia, Uppsala, Sweden). Plasma GLuc activity was quantified using a Renilla Luciferase Assay System (Promega, Madison, WI, USA). Plasma β‐hydroxybutyrate concentrations were measured using a β‐hydroxybutyrate Colorimetric Assay Kit (BioVision Inc., Milpitas, CA, USA). Plasma concentrations of free fatty acids were determined by NEFA C‐Test‐Wako kits (Fujifilm Wako Pure Chemical Co.). Hepatic triglyceride and glycogen contents were measured as described previously10, 26.
+ Open protocol
+ Expand
For intraperitoneal glucose tolerance tests, mice were intraperitoneally administered 1 g/kg body weight glucose for HFD-fed mice and 2 g/kg body weight glucose for NC-fed mice, with or without continuous intravenous administration of somatostatin (3 μg/kg/min) (Sigma) through a jugular catheter from 1 h before glucose administration. Blood glucose levels were measured using a GLUCOCARD G+ Meter (Arkray, Kyoto, Japan) and a commercial colorimetric kit (Wako). Plasma insulin levels were measured using a Mouse Insulin ELISA Kit (Shibayagi, Gunma, Japan).
+ Open protocol
+ Expand
Serum levels of glucose, lipids, and hormones were measured using appropriate equipment, reagents, and kits. The GLUCOCARD G+ meter was used to measure glucose content (Arkray, Kyoto, Japan). The NEFA C-Test Wako (Wako Pure Chemical Industries, Osaka, Japan) was used to measure free fatty acid levels. The Triglyceride E-Test Wako (Wako Pure Chemical Industries) was used to measure triglyceride levels and the Cholesterol E-Test Wako (Wako Pure Chemical Industries) for cholesterol content. The Rebis Insulin-rat T ELISA kit (Shibayagi, Gunma, Japan) was used to measure insulin levels, the Leptin ELISA kit (Morinaga Institute of Biological Science, Yokohama, Japan) for leptin, and the Corticosterone ELISA kit (Assaypro, St. Charles, MO) for corticosterone. Blood chemistry is listed in Tables 1 and 2.
+ Open protocol
+ Expand
Blood glucose levels were determined in tail blood using a Glucocard G+ meter (Arkray, Kyoto, Japan). Serum concentrations of total cholesterol, triglycerides, and non-esterified fatty acids were measured using commercial kits (LabAssay, Fujifilm Wako Pure Chemical, Osaka, Japan).
+ Open protocol
+ Expand
Serum levels of glucose, insulin, and lipids were measured using appropriate equipment, reagents, and kits. Glucose content was measured using a GLUCOCARD G+ meter (Arkray, Kyoto, Japan). The LBIS Insulin-mouse T ELISA kit (Shibayagi, Gunma, Japan) was used to measure insulin levels. The NEFA C-Test Wako (Wako Pure Chemical Industries, Osaka, Japan) was used to measure free fatty acid abundance. Triglyceride E-Test Wako (Wako Pure Chemical Industries) was used to measure triglyceride levels, and the Cholesterol E-Test Wako (Wako Pure Chemical Industries) was used to assess cholesterol content.
+ Open protocol
+ Expand
Twelve-week-old C57BL/6J mice were divided into 4 groups (n = 4 per group) and intraperitoneally injected with 5 µL/g body weight of saline with or without metformin hydrochloride (150, 300, or 600 mg/kg body weight) at ZT 0. Chow was taken away and after 2 h (at ZT 2), blood glucose level in the blood (taken from the tail) was measured using a Glucocard G+ meter (Arkray, Kyoto, Japan), and the mice were euthanized to obtain liver samples.
+ Open protocol
+ Expand
Each participant was provided with a GLUCOCARD G+ Meter (Arkray Inc., Kyoto, Japan). MEQNET™ SMBG Viewer software (Arkray Inc., Kyoto, Japan) was used as the data management software. The SMBG data were downloaded to the software during hospital visits, and the mean SMBG frequency (times/day) for a 30-day period was calculated from the downloaded data.
+ Open protocol
+ Expand
The GLUCOCARD G+ meter was used to measure the serum glucose content (Arkray, Kyoto, Japan). The Rebis Insulin-Mouse T ELISA Kit (Shibayagi, Gunma, Japan) was used to measure the serum insulin levels.
+ Open protocol
+ Expand
Blood glucose levels were measured using a GLUCOCARD G+ Meter (Arkray, Kyoto, Japan). Plasma insulin concentrations were determined using a mouse insulin enzyme‐linked immunosorbent assay kit (Wako, Saitama, Japan). Plasma aspartate aminotransferase/alanine aminotransferase (AST/ALT) levels were measured using the Transaminase CII‐Test‐Wako kit (Wako). Liver triglyceride concentrations were measured by using the TG E‐Test‐Wako (Wako), as described previously20. Liver tissues were fixed in 4% paraformaldehyde/phosphate‐buffered saline (Wako), and the sections were stained with Sirius red.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!