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Bs 23748r

Manufactured by Bioss Antibodies
Sourced in China
About the product

Bs-23748R is a high-quality lab equipment product designed for use in scientific research applications. It is a precise and reliable instrument that serves a core function in laboratory workflows. The detailed specifications and intended use of this product are not available in an unbiased and factual format within the constraints provided.

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2 protocols using «bs 23748r»

1

Tumor Immunohistochemical Analysis

2020
Tumour sections were cut at a thickness of 5 μm using a microtome and mounted on glass microscope slides. For immunohistochemical staining, sections were dewaxed in xylene and hydrated in graded alcohol solutions and distilled water. Routine haematoxylin and eosin (H&E) staining was carried out. Immunohistochemical staining was performed using antibodies against the proto-oncogene MDM2 (bs23748R, Bioss, China) and proliferation marker Ki67 (15580, Abcam) according to the manufacturers' recommendations.
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2

Western Blot Analysis of Cell Signaling Proteins

2019
The cell lysates were separated by 10–15% sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE) and electrophoretically transferred to a PVDF membrane (Millipore, Billerica, MA). The membrane was incubated with primary antibodies, including rabbit anti-ZBTB48 (Abcam, ab50588, Cambridge, United Kingdom), rabbit anti-GAPDH (Proteintech Co., 10494-1-AP, Wuhan, China), rabbit anti-P21 (Proteintech, 10355-1-AP), rabbit anti-P53 (Proteintech, 10442-1-AP), rabbit anti-CDKN2A/P16-INK4a (Bioss Co., bs-4592R, Beijing, China), rabbit anti-P14ARF (Bioss, bs0534R), rabbit anti-MDM2 (Bioss, bs-23748R), and rabbit anti-PPAR gamma (Bioss, bs-4590R) antibodies overnight at 4 °C, followed by incubation with an HRP-labeled goat or mouse anti-rabbit IgG for 1 h at room temperature. Specific proteins were detected by a high-sensitivity chemiluminescence imaging system (BioRad Laboratories, Hercules, CA, United States). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a control.
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