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Enzyme linked immunosorbent assay kit

Manufactured by Assaypro
Sourced in United States

The Enzyme-linked immunosorbent assay (ELISA) kit is a laboratory tool used to detect and quantify specific proteins, hormones, antibodies, or other molecules in a sample. The kit utilizes antibodies and color change to measure the concentration of the target analyte.

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11 protocols using enzyme linked immunosorbent assay kit

1

Immune Thrombocytopenia Treatment Outcomes

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Patient data, including age, gender, height, weight, treatment protocol, ITP-specific bleeding score (bleeding symptoms were scored according to the ITP bleeding scale) [16 (link)], expression of platelet glycoprotein-specific antibodies, treatment response and adverse reactions were retrospectively collected.
The complete blood count, including the platelet count, was measured by a haematology analyzer (Mindray Biomedical Electronics Co., Ltd., Shenzhen, China). The serum platelet glycoprotein-specific antibodies were determined using enzyme-linked immunosorbent assay kits in accordance with the manufacturer’s instructions (Assaypro LLC, Saint Charles, MO).
The patient response within three months of the treatment onset was evaluated as complete response (CR), response (R) or no response (NR). CR was defined as a platelet count ≥100 × 109/L without bleeding manifestations; R was defined as a platelet count ≥30 × 109/L or a two-fold increase over baseline platelet count without bleeding; and NR was defined as a platelet count <30 × 109/L or < two-fold increase of baseline platelet count or bleeding. The overall response = CR + R, and the time to response (TTR) was defined as time from treatment onset to response.
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2

Measuring Coagulation Factors by ELISA

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All fasting plasma samples were separated from EDTA anticoagulation whole blood after centrifuged in the first follow‐up (2013) and stored at −80℃ until 2019. Plasma samples of case‐control pairs were random and laboratory personnel was blinded to disease status. We carried out experiments according to the instruction of the enzyme‐linked immunosorbent assay kits, and measured FX and FXI levels on the multifunctional enzyme marker (BioTek) at 450 nm. Samples of quality control were to estimate the stability of experimental operation in all enzyme‐linked immunosorbent assay kits (Assaypro). Each sample and its duplicate were measured at the same time.
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3

Adipsin Quantification by ELISA

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Enzyme‐linked immunosorbent assay kits (AssayPro, St. Charles, MO, USA) were used to detected circulating adipsin. The standard linear range was 0.001875–0.120 ug/mL, and variations were less than 10%.
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4

Corticosterone Measurement in Cancer-Induced Anesthesia

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At the time of euthanasia, the animals were anesthetized by intraperitoneal injection, with a mixture of medetomidine, midazolam, and butorphanol. Blood samples were collected from the axillary artery from 10:00 to 11:00 a.m. on day 49 after cancer-cell inoculation. The serum was obtained by centrifugation of the blood sample at 3000 rpm for 15 min at 4 °C. The corticosterone level in the serum was measured using an enzyme-linked immunosorbent assay kit (Assaypro, St. Charles, MO, USA), following the manufacturer’s instructions [24 (link)].
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5

Quantifying t-PA in EA.hy926 Cells

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The conditioned medium of EA.hy926 cells transfected with siRNA or treated with sulforaphane (10 µM) (LKT Laboratories, St. Paul, MN, USA) in 24-well culture plates was used to determine t-PA using an enzymelinked immunosorbent assay kit (Assaypro LLC, St. Charles, MO, USA), after which the cell layer was analyzed for DNA content using fluorometry (Kissane and Robins, 1958) to normalize the t-PA content in the conditioned medium per µg DNA.
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6

Adiponectin Measurement Protocol

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Serum adiponectin levels were determined using a commercial enzyme-linked immunosorbent assay kit from AssayPro (St. Charles, MO, USA). The intra- and inter-assay coefficients of variation were 3.0% and 8.3%, respectively. The MDD of adiponectin was 0.781–50 ng/mL. The blood samples used to measure adiponectin levels were obtained after recruitment and were immediately centrifuged and stored at −70 °C until use.
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7

Tooth Loss Impacts Stress Hormones in AD Mice

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To investigate the effects of tooth loss on changes in the circulating stress hormone levels in AppNL − G − F mice, we measured the plasma corticosterone levels in 6-month-old mice (n = 10/group). Mice were injected intraperitoneally with the anesthetic mixture used for the tooth extraction surgery.
Blood samples were collected from the heart and centrifuged at 3500 rpm for 10 min at 4°C to separate the plasma. The plasma corticosterone levels were determined using an enzyme-linked immunosorbent assay kit according to the manufacturer’s instructions (Assaypro, St. Charles, MO, USA).
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8

Arsenite Modulates Endothelial Cell Secretion

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The conditioned medium of endothelial EA.hy926 cells treated with 1, 2, 5, or 10 µM arsenite for 24 h at 37 °C in a 24-well culture plate was used to measure t-PA and PAI-1 secretion with an enzyme-linked immunosorbent assay kit (AssayPro LLC, St. Charles, MO, USA), after which the cell layer was analyzed for DNA content using a fluorometric method [58 (link)] to normalize the t-PA and PAI-1 content in the conditioned medium per µg DNA. Separately, the conditioned medium of endothelial EA.hy926 cells treated with arsenite for 24 h was used to assess PGI2 secretion measured as 6-keto PGF with a 6-keto PGF enzyme-linked immunosorbent assay kit (Cayman Chemical Co., Ann Arbor, MI, USA). The accumulated 6-keto PGF in the conditioned medium was expressed as pg/µg DNA.
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9

Serum Biomarkers in Blood Sample Assessment

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5 cc venous blood was collected from every case using standard venipuncture aseptic technique. 1 cc taken on EDTA tube for complete blood picture assessment and the rest was centrifuged and sera were collected and stored at -20 for assessment of serum adiponectin, serum resistin, serum MCP-1 & total serum IgE using Enzyme linked immuno-sorbent assay (ELISA) technique.

• Serum IgE (immunoglobulin E): enzyme immunoassay test kit provided by Bio Check, Inc.; Foster city, CA 94404, USA; for the quantitative determination of IgE concentration in human serum)

• Serum resistin: Enzyme-linked immunosorbent assay kit, for quantitative detection of human resistin, provided by Assaypro LLC 30 Triad South Drive St. Charles, MO 63304, USA)

• Serum adiponectin: ELISA kit, for quantitative sandwich enzyme immunoassay technique that measures adiponectin in less than 3 hours, provided by Assaypro LLC 30 Triad South Drive St. Charles, MO 63304, USA).

• Serum MCP-1: ELISA kit, for quantitative determination of human MCP-1 in human serum provided by glory science co., ltd. 2400 veterans Blvd. Suite 16 – 101, Del Rio, TX 78840, USA).

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10

Biochemical Analyses of Plasma and Liver

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EDTA plasma further served for the analysis of albumin as a parameter of liver function using a commercially available enzyme-linked immunosorbent assay kit in accordance with the manufacturer’s instructions (Assaypro, MO, USA). Measurements of plasma triglycerides (TG) and free fatty acids (FFA), serving as indicators of systemic dyslipidemia, were performed using the TG (Cayman Chemical Company, MI, USA; 10010303) and FFA (abcam, ab65641) assay kit methods according to the manufacturer’s instructions. Glycogen levels in liver samples were measured using a glycogen assay kit (Abnova; KA0861) according to the manufacturer’s instructions with 10 mg of homogenized frozen liver tissue. For measurement of hepatic TG, Lipids were extracted from mouse liver biopsies using the commercially available LabAssay Triglyceride (WAKO Pure Chemicals, Kyoto, Japan).
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