Immediately after LCM, RNA was extracted from the respective cartilage zones using an RNeasy Micro kit (Qiagen GmbH, Hiden, Germany) with routine use of DNase I (Qiagen). cDNA was synthesized using Sensiscript reverse transcriptase (Qiagen). The gene expression was evaluated quantitatively by real-time PCR on a LightCycler (Roche Diagnostics, Basel, Switzerland), using gene-specific primers and probes (Additional file
PixCell IIe
The PixCell IIe is a laser-based microdissection system designed for precise and efficient cell isolation. It features a high-precision laser capture mechanism that allows for the selective extraction of targeted cells or tissue samples from heterogeneous samples. The PixCell IIe is a laboratory instrument used for micromanipulation and microscale sample preparation.
Lab products found in correlation
6 protocols using PixCell IIe
Laser Capture Microdissection of Osteoarthritic Cartilage
Immediately after LCM, RNA was extracted from the respective cartilage zones using an RNeasy Micro kit (Qiagen GmbH, Hiden, Germany) with routine use of DNase I (Qiagen). cDNA was synthesized using Sensiscript reverse transcriptase (Qiagen). The gene expression was evaluated quantitatively by real-time PCR on a LightCycler (Roche Diagnostics, Basel, Switzerland), using gene-specific primers and probes (Additional file
Laser Capture of IPMN Cells for Mitochondrial DNA Analysis
Isolation and Analysis of B Cell Lineage from CNS Tissue
Transcriptomic Analysis of Chlorine-Exposed Mouse Trachea
Laser Capture Microdissection and Whole Genome Amplification
Laser Capture Microdissection Protocol
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