The ethanol extract was obtained by soaking 468.43g of blended A. sativum in 1000ml of ethanol and shaken intermittently for 72hours. The mixture was first sieved with cheese cloth and then with Whatmann filter paper number: 42 (125mm). The solution was concentrated using rotary evaporator and the crude extract was obtained and weighed.
Whatmann filter paper number 42
Manufactured by Cytiva
3 citations
About the product
Whatman filter paper number 42 is a quantitative grade filter paper designed for general filtration purposes. It has a medium-slow filtration rate and retention of particles down to 2.5 microns. The paper is made from high-quality cellulose fibers and is available in a range of sizes to suit various laboratory applications.
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Get pricing insights and sourcing options3 protocols using «whatmann filter paper number 42»
1
Garlic Ethanol Extract Preparation
2
Methanolic Extraction of O. hispidum Roots
O. hispidum roots were shade-dried and macerated in methanol at room temperature three times for 21, 7, and 3 days respectively. Whatmann filter paper number 42 with a pour size of 2 mm and muslin cloth were used to filter the extracted material. Using a rotary evaporator, the filtrate was concentrated at 35–40 °C under reduced pressure of −220 mm Hg (Williamson et al., 1996 ).
Corresponding organizations : COMSATS University Islamabad
3
Extraction and Characterization of n-Hexane from Leaves
The leaves were air-dried at room temperature for two weeks, after which they were ground to a uniform powder. The n-hexane extract was prepared by soaking 100 g of the dried ground material in 1 L of n-hexane at room temperature for 48 hrs. The extract was filtered after 48 hrs through a Whatmann filter paper number 42 (125 mm). The extract was then concentrated using a rotary evaporator in a water bath at a temperature not exceeding 40°C. The extract was reconstituted in DMSO at 10 mg/mL for all bioassays.
Corresponding organizations : University of the Free State, Institute of Biomedical Science, Tshwane University of Technology, BioClinica (United States), Onderstepoort Veterinary Academic Hospital, University of Pretoria, University of South Africa
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