The leaves were air-dried at room temperature for two weeks, after which they were ground to a uniform powder. The n-hexane extract was prepared by soaking 100 g of the dried ground material in 1 L of n-hexane at room temperature for 48 hrs. The extract was filtered after 48 hrs through a Whatmann filter paper number 42 (125 mm). The extract was then concentrated using a rotary evaporator in a water bath at a temperature not exceeding 40°C. The extract was reconstituted in DMSO at 10 mg/mL for all bioassays.
Whatmann filter paper number 42
Manufactured by Cytiva
Whatman filter paper number 42 is a quantitative grade filter paper designed for general filtration purposes. It has a medium-slow filtration rate and retention of particles down to 2.5 microns. The paper is made from high-quality cellulose fibers and is available in a range of sizes to suit various laboratory applications.
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Lab products found in correlation
2 protocols using whatmann filter paper number 42
1
Extraction and Characterization of n-Hexane from Leaves
2
Methanolic Extraction of O. hispidum Roots
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O. hispidum roots were shade-dried and macerated in methanol at room temperature three times for 21, 7, and 3 days respectively. Whatmann filter paper number 42 with a pour size of 2 mm and muslin cloth were used to filter the extracted material. Using a rotary evaporator, the filtrate was concentrated at 35–40 °C under reduced pressure of −220 mm Hg (Williamson et al., 1996 ).
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