Hxc apo

Manufactured by Leica

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About the product

The HXC APO is a high-performance apochromatic lens designed for microscopy applications. It offers excellent optical performance with low chromatic aberration and high resolution. The lens is suitable for a variety of microscopy techniques, but a detailed description of its intended use is not available.

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Lab products found in correlation

Dmi6000 cs, by Leica (3 mentions) Easy3d module, by Abberior (3 mentions) Cube and box, by Life Imaging Services (3 mentions) Imspector software, by Abberior (2 mentions) Ust 2 solid universal joint, by Narishige (1 mentions) Tcs sp8 dm6000 cfs confocal microscope, by Leica (1 mentions) Confocal software version 2.61, by Leica (1 mentions) Viscotears, by Novartis (1 mentions) Fluorescein isothiocyanate fitc labeled dextran, by Merck Group (1 mentions) Sg 4n, by Narishige (1 mentions) Inspector software, by Abberior (1 mentions)

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Spelling variants (same manufacturer)

HXC PL APO 63x/1.40 OIL CS - 3 citations HXC APO 20× 0.5 W - 2 citations

The spelling variants listed below correspond to different ways the product may be referred to in scientific literature.
These variants have been automatically detected by our extraction engine, which groups similar formulations based on semantic similarity.

4 protocols using «hxc apo»

3D-STED Microscopy Setup and Characterization

2023

We used a home-built 3D-STED setup (for details, see Inavalli et al., 2019) (link) constructed around an inverted microscope body (DMI 6000 CS, Leica Microsystems), which was equipped with a TIRF oil objective (x100, 1.47 NA, HXC APO, Leica Microsystems) and a heating box (Cube and Box, Life Imaging Services) to maintain a stable temperature of 32°C. A pulsed-laser (PDL 800-D, PicoQuant) was used to deliver excitation pulses (90 ps at 80 MHz) at 485 nm and a synchronized de-excitation laser (Onefive Katana 06 HP, NKT Photonics) operating at 592 nm was used to generate the STED light pulses (500-700ps). The STED beam was reflected on a spatial light modulator (Easy3D Module, Abberior Instruments) to generate a mixture of doughnut-and bottle-shaped beams for 2D and 3D-STED respectively. Image acquisition was controlled by the Imspector software (Abberior Instruments). The performance and spatial resolution of the microscope was checked and optimized by visualizing and overlapping the PSFs of the laser beams using 150 nm gold nano-spheres and correcting the main optical aberrations.
Usually, the spatial resolution was 175 nm (lateral) and 450 nm (axial) in confocal mode and 60 nm (lateral) and 160 nm (axial) in STED mode.

Idziak A., Inavalli V.V.G.K., Bancelin S., Arizono M., & Nägerl U.V. (2023). The impact of chemical fixation on the microanatomy of mouse brain tissue.

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3D Super-Resolution Microscopy with Home-Built STED Setup

2023

We used a home-built 3D-STED setup (for details, see Inavalli et al., 2019 (link)) constructed around an inverted microscope body (DMI 6000 CS, Leica Microsystems), which was equipped with a TIRF oil objective (100×, 1.47 NA, HXC APO, Leica Microsystems) and contained within a heating box (Cube and Box, Life Imaging Services) to maintain a stable temperature of 32°C. A pulsed-laser (PDL 800-D, PicoQuant) was used to deliver excitation pulses (90 ps at 80 MHz) at 485 nm and a synchronized de-excitation laser (Onefive Katana 06 HP, NKT Photonics) operating at 592 nm was used to generate the STED light pulses (500–700 ps). The STED beam was reflected on a spatial light modulator (SLM, Easy3D Module, Abberior Instruments) to generate a mixture of doughnut-shaped and bottle-shaped beams for 2D and 3D-STED, respectively. Image acquisition was controlled by the Imspector software (Abberior Instruments). The performance and spatial resolution of the microscope was checked and optimized by visualizing and overlapping the PSFs of the laser beams using 150-nm gold nano-spheres and 40-nm fluorescent beads and correcting the main optical aberrations with the SLM. The spatial resolution was 175 nm (lateral) and 450 nm (axial) in confocal mode and 60 nm (lateral) and 160 nm (axial) in STED mode.

Idziak A., Inavalli V.V., Bancelin S., Arizono M, & Nägerl U.V. (2023). The Impact of Chemical Fixation on the Microanatomy of Mouse Organotypic Hippocampal Slices. eNeuro, 10(9), ENEURO.0104-23.2023.

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Super-Resolution Microscopy Setup

2023

We used a custom-built STED/confocal setup^{55 (link)} constructed around an inverted microscope body (DMI 6000 CS, Leica Microsystems) which was equipped with a TIRF oil objective (x100, 1.47 NA, HXC APO, Leica Microsystems) and a heating box (Cube and Box, Life Imaging Services) to maintain a stable temperature of 32 °C. A pulsed-laser (PDL 800-D, PicoQuant) was used to deliver excitation pulses at 488 nm and a de-excitation laser (Onefive Katana 06 HP, NKT Photonics) operating at 594 nm was used to generate the STED light pulses. The STED beam was profiled to a donut shape using a spatial light modulator (Easy3D Module, Abberior Instruments). Image acquisition was controlled by the Inspector software (Abberior Instruments). The spatial resolution of the microscope was 175 nm (x-y) and 450 nm (z) in confocal mode and 60 nm (x-y) and 160 nm (z) in STED mode.

Valiente-Gabioud A.A., Garteizgogeascoa Suñer I., Idziak A., Fabritius A., Basquin J., Angibaud J., Nägerl U.V., Singh S.P, & Griesbeck O. (2023). Fluorescent sensors for imaging of interstitial calcium. Nature Communications, 14, 6220.

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Intravital Imaging of Transplanted Islets

2019

At the indicated time points after transplantation, mice were anesthetized with a 30% oxygen and a ~2% isoflurane mixture and placed on a heating pad. We restrained the mouse head with a stereotaxic head-holder (SG-4N, Narishige) and positioned the eye containing the engrafted islets facing upwards. The eyelid was carefully pulled back to hold the eye gently at the coreneoscleral junction with a pair of tweezers attached to a UST-2 Solid Universal Joint (Narishige). The tips of the tweezers were covered with a single piece of polythene tubing, creating a loop between the two tips. This arrangement permitted a flexible but stable fixation of the head and eye without causing damage or disrupting the blood circulation in the eye. Imaging was performed using an upright Leica TCS SP8 DM6000 CFS confocal microscope (Leica Microsystems) equipped with White Light Laser (470–670 nm) using a long-distance water dipping lens (Leica HXC APO 10 × 0.3 w)^{28 (link)}. Viscotears (Novartis) was used as an immersion medium between the lens and the mouse eye. Backscatter signal imaging of each islet was obtained using a 633 nm laser beam as previously described^{29 (link)}. To visualize the blood vessels, the animal was injected with 150 kDa Fluorescein isothiocyanate (FITC)-labeled dextran (20 mg/kg, Sigma) via the retro-orbital venous sinus. Fluorescence emission from FITC was obtained by excitation at 488 nm and detection between 500 and 550 nm. No signs of photo-damage in islet cells were observed. Leica Confocal Software (version 2.61), and ImageJ were used to process images.

Fan Y., Zheng X., Ali Y., Berggren P.O, & Loo S.C. (2019). Local release of rapamycin by microparticles delays islet rejection within the anterior chamber of the eye. Scientific Reports, 9, 3918.

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