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AM157S-K

Manufactured by Asan Pharmaceutical

The AM157S-K is a laboratory equipment product. It is a device designed for use in scientific research and analysis. The core function of the AM157S-K is to perform specific tasks required in a laboratory setting. No further details can be provided while maintaining an unbiased and factual approach.

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7 protocols using AM157S-K

After 9 weeks of experimental period, all the mice were subjected to fasting for 16 h and anesthetized with isoflurane. Before tissue harvest, blood was collected by cardiac puncture into serum separation tubes (BD Microtainer ® tube, 365967, BD Biosciences, Franklin Lakes, NJ, USA), clotted for 30 min at RT, and centrifuged at 21,206 × g at 4°C for 90 s to isolate the serum. The serum was stored at −70°C until further analysis. Assay kits for determining serum levels of glucose (AM201-K), triacylglycerol (TG; AM-157S-K), total cholesterol (TC; AM-157S-K), and high-density lipoprotein cholesterol (HDL-C; AM-202-K) were purchased from Asan Pharmaceutical Co. (Seoul, Korea). Serum levels were determined using each commercial kit according to the manufacturer’s instructions. Low-density lipoprotein cholesterol (LDL-C) levels were calculated using the Friedewald equation (22 (link)). After collecting the blood, epididymal adipose tissue was rapidly removed from the mice and weighed immediately. The tissues were snap-frozen in liquid nitrogen and stored at −70°C until analysis.
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A commercially available assay kit was used to quantify triglyceride levels (AM 157S-K, Asan Pharmaceutical, Seoul, Korea). In this assay, triglycerides are converted to free fatty acids and glycerol, which is then oxidized to form a product that reacts with a probe to produce a colored product whose concentration can be determined by spectrophotometry. In particular, samples were incubated with enzyme mix at 37℃ for 10 minutes before measured absorbance at a wavelength of 550 nm using microplate reader (Spectra Max 190).
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Fresh softshell turtle (T. sinensis) bodies were obtained from the Gunwi Zara Aquaculture Farm (Ubo, Gunwi, Korea). A voucher specimen is deposited in the laboratory of Dr. Mi-Ryung Kim (Silla University, Busan, Korea). Whole bodies (2 kg) were extracted with boiling water (20 L) for 25 h. After removing the residue and oily layer by centrifugation at 3,000 g for 10 min, the aqueous portion was concentrated using a rotary evaporator to obtain the extract (220 g; yield 11%). The extract was adjusted to 88 mg/mL (or 50 mg/mL as protein) with distilled water. Bovine serum albumin was used as the standard when determining the protein content (8 (link)). Assay kits for determining glucose (AM201-K), urea (AM165-K), lactate dehydrogenase (LDH; LDH-LQ), triglyceride (AM157S-K), and cholesterol (AM202-K) were purchased from Asan Pharmaceutical (Seoul, Korea). The glutathione peroxidase (GPx) kit (K762-100) was purchased from BioVision (Milpitas, CA, USA). The other reagents used in this study were of analytical grade.
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Briefly, 1.0–1.2 ml total blood was collected from each mouse using cardiac puncture following sacrifice. The collected blood was immediately centrifuged (1,000 × g for 30 min at 4°C) to obtain plasma. The plasma levels of triglycerides (TG) and total cholesterol (TC) were measured using commercial kits (AM157S-K for TG and AM 202-K for TC) (Asan Pharmaceutical, Co., Ltd.).
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Total lipids were isolated from 250 mg of mouse liver in a chloroform-methanol mixture (2:1, v/v). The total lipid extract was measured enzymatically using a commercially available enzymatic TG assay kit (AM157S-K; Asan Pharmaceutical, Seoul, Korea) according to the manufacturer’s protocol. TG concentration was measured colorimetrically at 532 nm using a MULTISKAN GO reader (Thermo Scientific, Waltham, MA, USA).
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Blood glucose levels were measured by Accu-Chek Aviva glucose monitors (Roche Diagnostics, Indianapolis, IN, USA) and plasma insulin was measured using an ELISA kit (cat. no. EZRMI-13K; Millipore, Bedford, MA, USA). Plasma levels of total cholesterol (TC), triglyceride (TG) and HDL-cholesterol were measured using commercially available kits (cat. no’s. AM202-K, AM157S-K and AM203-K, respectively; Asan Pharmaceutical, Seoul, Korea). For liver TG quantification, liver tissues were homogenized and extracted in chloroform, methanol and DW (2/1/1 ratio).
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Blood samples were collected in tubes and immediately centrifuged at 900× g for 10 min at 4 °C. ALT, AST, and TG levels were measured using measurement kits (Cat No. AM102-k, AM103-k, and AM157S-k; Asan Pharmaceutical, Seoul, Republic of Korea).
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