NANO-100 micro-spectrophotometer
The NANO-100 micro-spectrophotometer is a compact and precise instrument designed for the measurement of absorbance and transmittance spectra of small sample volumes. It features a wavelength range of 190 to 1100 nanometers and a wavelength accuracy of ±0.5 nanometers. The NANO-100 is equipped with a high-performance detector and a user-friendly software interface for data acquisition and analysis.
Lab products found in correlation
6 protocols using NANO-100 micro-spectrophotometer
Quantifying Gene Expression in bEnd.3 Cells
Profiling M2 Macrophage and Glycolysis Markers
Primers information
Gene name | Primers sequences | Species |
---|---|---|
18SrRNA | F: 5ʹ-ATGCGGCGGCGTTATTCC-3’ | Mouse |
F: 5ʹ-GCTATCAATCTGTCAATCCTGTCC-3’ | ||
IFI27 | F: 5ʹ-GACTCTCCGTGCCATCTACTG-3’ | Mouse |
R: 5ʹ-CCTCTATCGCCATATCTGCCAC-3’ | ||
CD163 | F: 5ʹ- CTGGCGGGTGGTGAAAACA-3’ | Mouse |
R: 5ʹ- CAGCCGTTACTGCACACTG-3’ | ||
CD206 | F: 5ʹ- GAGGGAAGCGAGAGATTATGGA-3’ | Mouse |
R: 5ʹ- GCCTGATGCCAGGTTAAAGCA-3’ | ||
HK2 | F: 5ʹ- TGATCGCCTGCTTATTCACGG-3’ | Mouse |
R: 5ʹ- AACCGCCTAGAAATCTCCAGA-3’ | ||
LDHA | F: 5ʹ-TGTCTCCAGCAAAGACTACTGT-3’ | Mouse |
R: 5ʹ-GACTGTACTTGACAATGTTGGGA-3’ | ||
PGK1 | F: 5ʹ-ATGTCGCTTTCCAACAAGCTG-3’ | Mouse |
R: 5ʹ-GCTCCATTGTCCAAGCAGAAT-3’ | ||
ENO1 | F: 5ʹ-TGCGTCCACTGGCATCTAC-3’ | Mouse |
R: 5ʹ-CAGAGCAGGCGCAATAGTTTTA-3’ |
Quantitative RT-PCR for Gene Expression
Quantitative Gene Expression Analysis
Resting and Activated CD8+ T Cell DNA Extraction
Resting and activated mature CD8þ T cells were harvested and washed once in phosphate-buffered saline (PBS). DNA preparation was performed using a High Pure PCR Template Preparation Kit (Indianapolis, Indiana). Briefly, 200 μl of sample material was added to 200 μl of binding buffer and 40 μl proteinase K. The resulting mixture was incubated at 70°C for 10 min. After adding 100 μl isopropanol, the sample was loaded into high filter tubes. After centrifugation, 500 μl inhibitor removal buffer was added. The samples were centrifuged and washed twice with 500 μl wash buffer. Prewarmed elution buffer was added in order to elute the DNA. The concentration of DNA was calculated using a Nano-100 Micro-spectrophotometer (Allsheng, Hangzhou city, China).
RNA Extraction and qRT-PCR Analysis
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