Mda mb 231

MCF10A, ZR75-30, MDA-MB-453, BT20, JIMT-1, MDA-MB-231, and HEK293T cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). MCF7, T47D, ZR75-1, SKBR3, BT474, HCC-1954, HCC1419, and HS578T cells were purchased from the Korean Cell Line Bank. MCF7, T47D, MDA-MB-231, and HEK293T cells were cultured in DMEM (Welgene, Daegu, Republic of Korea) supplemented with 10% FBS, and SKBR3, BT474, HCC-1954, HCC-1419, JIMT-1, and HS5788T cells were cultured in RPMI (Welgene) supplemented with 10% FBS at 37 °C in a 5% CO2 atmosphere. Trastuzumab was obtained from Roche (Basel, Switzerland). The β-Catenin/TCF inhibitor FH-535 was purchased from Sigma-Aldrich (St. Louis, MO, USA).

Human breast cancer cell lines (MDA-MB-231 and MCF7) and NSCLC cell lines (H460 and H226B cells) were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA) or kindly provided by Dr. John V. Heymach (MD Anderson Cancer Center, Houston, TX, USA). T47D cells were kindly provided by Dr. Sang Kook Lee (Seoul National University, Seoul, Korea). MDA-MB-453, BT-20, and MDB-MB-468 cells were kindly provided by Dr. Hyeong-Gon Moon (Seoul National University, Seoul, Korea). The cells were cultured in DMEM (MDA-MB-231, MCF7, MDA-MB-453, and MDA-MB-468 cells) or RPMI 1640 medium (H460, H226B, T47D, and BT-20 cells) supplemented with 10% fetal bovine serum (FBS) and antibiotics (Welgene, Kyeongsan-si, Korea), and maintained at 37 °C with 5% CO₂ in a humidified atmosphere. Paclitaxel-resistant MDA-MB-231 (MDA/R) cells were generated by continuously exposing the cells to increasing concentrations of paclitaxel for more than 6 months. These cancer cell lines were authenticated and validated using an AmplFLSTR identifier PCR Amplification kit (Applied Biosystems, Foster, CA, USA; cat. no. 4322288) in 2013 and 2017. We used cell lines passed for fewer than three months.

The human breast cancer cell lines MCF-7 (ER-positive subtype), MDA-MB-231 and HCC-38 (TN subtype) were obtained from the Korean Cell Line Bank (Seoul, Korea). Hs578T cells (TN subtype) were obtained from ATCC (Manassas, VA, USA). MCF-7 cells were grown in Dulbecco’s Modified Eagle’s Medium (DMEM) (WelGENE, Daegu, Korea) containing 10 % fetal bovine serum (FBS) and supplemented with a 1 % antibiotic solution containing penicillin and streptomycin (Gibco, Auckland, NZ). The MDA-MB-231, HCC-38, and Hs578T cells were grown in Roswell Park Memorial Institute (RPMI) 1640 medium (WelGENE) containing 10 % FBS and supplemented with a 1 % antibiotic solution containing penicillin and streptomycin (Gibco). The MCF-7, MDA-MB-231, HCC-38, and Hs578T cells used in this study were authenticated and validated by DNA fingerprinting (AmplFLSTR identifiler PCR Amplification kit), which was conducted by the Korean Cell Line Bank.

Mouse mammary carcinoma cells (EO771, 4T1), human breast cancer cells (Hs578T, MDA-MB-231), mouse normal mammary epithelial cells (NMuMG), and human non-malignant breast epithelial cells (MCF10A) were purchased from American Type Culture Collection (ATCC). EO771, 4T1, Hs578T, and MDA-MB-231 breast cancer cells were maintained in monolayers in DMEM (WelGENE, Daegu, South Korea) with 10% fetal bovine serum (FBS; WelGENE, Daegu, South Korea), 1% penicillin/streptomycin (GIBCO, Grand Island, NY, USA). NMuMG cells were maintained in monolayers in DMEM (WelGENE) with 10% fetal bovine serum (FBS; WelGENE, Daegu, South Korea), 1% penicillin/streptomycin, and 10 μg/ml insulin (Sigma-Aldrich, St.Louis, MO, USA). MCF10A cells were maintained in DMEM/Ham’s F-12 nutrient mixture (GIBCO) with 5% horse serum (GIBCO), 20 ng/ml EGF (Peprotech), 10 μg/ml insulin, 0.5 μg/ml hydrocortisone (Sigma), and 100 ng/ml choleratoxin (Sigma). All cell lines were maintained at 37 °C in CO₂ humidified atmosphere. For all experiments cells were grown to 70–80% confluence. The cell lines in this study were routinely tested for mycoplasma contamination by PCR. Tetraarsenic hexoxide (As₄O₆, TetraAS^®) was provided by CHEMAS Co. Ltd. (Seoul, South Korea) N-Acetyl-Asp-Glu-Val-Asp-al (Ac-DEVD-CHO) and N-Acetyl-L-cysteine (NAC) were purchased from Sigma-Aldrich.

Human prostate cancer cell line PC3, human lung cancer cell line A549, and human breast cancer cell line MDA-MB-231 were purchased from the Korean Cell Line Bank (Seoul, Korea). Human glioma cells (U251, U373, and U87MG) were purchased from ATCC (American Type Culture Collection, Manassas, VA). U251 and MDA-MB-231 were maintained in Dulbecco's modified Eagle medium (DMEM, WELGENE, Gyeongsan, Korea). PC3 and A549 were maintained in Roswell Park MEMorial Institute 1640 medium (RPMI1640, WELGENE). U87MG and U373 were grown in Minimum Essential Medium (MEM, Gibco, Grand Island, NY). Each medium (DMEM, MEM, and RPMI) was supplemented with 10% (v/v) fetal bovine serum (FBS, Gibco) and 1% antibiotics containing penicillin/streptomycin (Invitrogen, Grand Island, NY). All cells were maintained at 37 °C in a humidified atmosphere with 5% CO₂.

MCF7, T47D, and MDA-MB-231 cell lines were obtained from American Type Culture Collection (Manassas, VA, USA). Palbociclib-resistant MCF7 and T47D cells (MCF7-PR and T47D-PR) cells were generated in the laboratory of the current study, as previously mentioned [9 (link)]. MCF7, T47D, and MDA-MB-231 cells were routinely maintained in RPMI 1640 medium (WelGENE Inc., Daegu, Korea) supplemented with 10% heat-inactivated fetal bovine serum (cat# S001-01; WelGENE Inc.) and 1% 100× penicillin/streptomycin solution (WelGENE Inc.). MCF7-PR and T47D-PR cells were maintained with 1.5 µM palbociclib concentration, and the drug was washed out for 24–48 h before experiments were performed.

Human mammary-gland-derived cell lines (MCF-10A, MCF-7, and MDA-MB-231) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). MCF-10A was cultured in MEGM (Lonza, Basel, Switzerland) with 100 ng/mL cholera toxin. MCF-7 and MDA-MB-231 were cultured in RPMI 1640 medium (Welgene, Seoul, Korea) supplemented with 10% fetal bovine serum (Capricorn Scientific, Ebsdorfergrund, Germany). All cells were supplemented with 2% penicillin/streptomycin (Capricorn Scientific) and cultured at 37 °C with 5% CO₂ in a humidified incubator.