Inspector software

Manufactured by Abberior

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About the product

The Inspector software is an image acquisition and analysis tool developed by Abberior. It is designed to control and operate various microscope systems and acquire high-quality images. The software provides a user-friendly interface for controlling the microscope hardware, configuring imaging parameters, and processing the acquired data. The core function of the Inspector software is to facilitate the efficient capture and management of microscopic images.

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Lab products found in correlation

Dmi6000 cs, by Leica (1 mentions) Easy3d module, by Abberior (1 mentions) Cube and box, by Life Imaging Services (1 mentions) Hxc apo, by Leica (1 mentions) Single photon counting detectors, by Excelitas (1 mentions) Alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Expert line gated sted, by Nikon (1 mentions) Paraformaldehyde (pfa), by Electron Microscopy Sciences (1 mentions) Glutaraldehyde, by Electron Microscopy Sciences (1 mentions) Anti rabbit 580 sted, by Abberior (1 mentions) Anti rat star red, by Abberior (1 mentions)

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InSpector software (by Miltenyi Biotec) - 4 citations

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3 protocols using «inspector software»

Multimodal Microscopy for Cellular Imaging

2023

The images were obtained using a combination of confocal and superresolution microscopy. DAPI and WGA 488 were acquired in confocal mode and images of MPO (STAR RED) and IL-40 (STAR 580) in cell detail were acquired using stimulated emission depletion (STED) microscopy. The imaging was performed on an Abberior Expert Line STED system (Abberior Instruments GmbH) equipped with a Nikon Eclipse Ti-E body (Nikon, Tokyo, Japan), QUAD beam scanner and Nikon CFI Plan Apo 60x/NA 1.40 oil-immersion objective (Nikon). The samples were illuminated with 405 nm, 485 nm, 561 nm and 640 nm lasers. The fluorescence excited with 561 and 640 nm laser was depleted by a pulsed 775 nm STED laser in 2D donut shape. The fluorescence signal passed the pinhole set to 1 AU, was filtered by emission filters (422–467 nm, 506–594 nm, 580–630 nm and 650–720 nm) and the signal was detected with single-photon counting detectors (Excelitas Technologies, Waltham, MA, USA). The images were obtained by the Inspector software (Abberior Instruments GmbH).

Navrátilová A., Bečvář V., Hulejová H., Tomčík M., Štolová L., Mann H., Růžičková O., Šléglová O., Závada J., Pavelka K., Vencovský J., Šenolt L, & Andrés Cerezo L. (2023). New pro-inflammatory cytokine IL-40 is produced by activated neutrophils and plays a role in the early stages of seropositive rheumatoid arthritis. RMD Open, 9(2), e002894.

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Super-Resolution Microscopy Setup

2023

We used a custom-built STED/confocal setup^{55 (link)} constructed around an inverted microscope body (DMI 6000 CS, Leica Microsystems) which was equipped with a TIRF oil objective (x100, 1.47 NA, HXC APO, Leica Microsystems) and a heating box (Cube and Box, Life Imaging Services) to maintain a stable temperature of 32 °C. A pulsed-laser (PDL 800-D, PicoQuant) was used to deliver excitation pulses at 488 nm and a de-excitation laser (Onefive Katana 06 HP, NKT Photonics) operating at 594 nm was used to generate the STED light pulses. The STED beam was profiled to a donut shape using a spatial light modulator (Easy3D Module, Abberior Instruments). Image acquisition was controlled by the Inspector software (Abberior Instruments). The spatial resolution of the microscope was 175 nm (x-y) and 450 nm (z) in confocal mode and 60 nm (x-y) and 160 nm (z) in STED mode.

Valiente-Gabioud A.A., Garteizgogeascoa Suñer I., Idziak A., Fabritius A., Basquin J., Angibaud J., Nägerl U.V., Singh S.P, & Griesbeck O. (2023). Fluorescent sensors for imaging of interstitial calcium. Nature Communications, 14, 6220.

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High-Resolution Imaging of Mitotic Spindle

2020

U2OS cells grown on glass coverslips were fixed using a solution of 4% paraformaldehyde (Electron microscopy sciences) and 0.1% glutaraldehyde (Electron microscopy sciences) for 10 min at RT. Autofluorescence was quenched using freshly prepared 0.01% sodium borohydride for 7 min at RT. Cells were permeabilized with 0.5% Triton-X100 in PBS for 10 min at RT, washed, and incubated in blocking solution (10% fetal bovine serum (FBS) in 0.05% PBS-Tween) for 30 min.
Immunolabelling was performed with the following primary antibodies: rabbit anti-CENP-E (1:25), rabbit anti-KIF4A (1:50; PA5-30492), rat anti-tyrosinated tubulin (1:100) and guinea pig anti-CENP-C (1:500), fluorescently labelled secondary antibodies anti-rabbit 580 STED (1:100; Abberior), anti-rat STAR RED (1:100, Abberior) and anti-guinea pig Alexa Fluor-488 (1:1000, Thermo Fisher Scientific) and DAPI (1 µg/mL) as DNA counterstain.CH-STED microscopy was performed as described previously (Pereira et al., 2019) . An Abberior Instruments 'Expert Line' gated-STED was used coupled to a Nikon Ti microscope with 60x 1.4NA Plan-Apo objective (Nikon, Lambda Series) oil-immersion, pinhole size of 0.8 Airy units, 40 MHz modulated excitation (405, 488, 560 and 640nm) and depletion (775nm) lasers. Abberior's Inspector software was used to control the acquisition process. CH-STED was always acquired after 2D-STED (and z-STED) acquisitions to avoid artifacts caused by photo-bleaching. siControl (49, 5), STLC (31, 3), siKIF15 (39, 3), siKID (32, 3), siKIF4A (28, 3), siKIF2A (36, 3), siCLASPs (21, 2), GSK923295 (44, 3); -monopolar spindles: siControl (35, 3), siKIF15 (44, 3), siKID (38, 3), siKIF4A (44, 3), siKIF2A (38, 3) , siCLASPs (12, 2), GSK923295 (33, 3). P-values were calculated using one-way ANOVA. n.s. -not significant, **** P ≤ 0.0001. N (number of cells, number of independent experiments): siControl (49, 5), siNDC80 (59, 6), siNDC80 + siKIF4A (41, 3), siNDC80 + STLC (bipol.) (28, 3), siNDC80 + siKIF15 (36, 4), siNDC80 + siCLASPs (33, 3), siNDC80 + siKIF2A (38, 4) , siNDC80 + GSK923295 (38, 3), early prometaphase (23, 3), early prometaphase + GSK923295 (28, 3). P-values were calculated using transfected with control or HSET 3′UTR-targeting siRNAs and co-transfected with the RNAiresistant GFP-HSET constructs. Expression levels of HSET was observed using anti-HSET antibody with α-tubulin serving as a loading control.

Steblyanko Y., Rajendraprasad G., Osswald M., Eibes S., Geley S., Pereira A.J., Maiato H., & Barišić M. (2020). Microtubule poleward flux in human cells is driven by the coordinated action of four kinesins.

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