Porcine fgf23 elisa kit

Q: What specific identification details should researchers use when referencing this ELISA kit?

When citing this kit in academic publications, include the manufacturer (Wuhan Fine Biotech), catalog number, lot number, and specific assay version. Recommended citation format includes the full product name: 'Porcine Fibroblast Growth Factor 23 (FGF23) Enzyme-Linked Immunosorbent Assay (ELISA) Kit'.

Q: What laboratory systems and research contexts are compatible with this FGF23 ELISA kit?

This ELISA kit is compatible with standard microplate readers and compatible with complementary endocrine research tools like intact PTH and osteocalcin ELISA kits from Immunodiagnostic Systems. Ideal for bone metabolism, kidney function, and mineral regulation research platforms.

Q: What primary scientific research domains utilize this Porcine FGF23 ELISA Kit?

This kit is extensively used in veterinary endocrinology, bone metabolism studies, renal disease research, and comparative physiology. Researchers studying phosphate homeostasis, chronic kidney disease, and mineral regulatory mechanisms frequently employ this specialized diagnostic tool.

Q: What fascinating scientific insight relates to FGF23 research?

FGF23 was discovered as a critical hormone regulating phosphate metabolism, challenging previous understanding of bone-kidney endocrine interactions. Its role in detecting early-stage mineral dysregulation represents a groundbreaking advancement in translational medical research.

Manufactured by Wuhan Fine Biotech

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About the product

The Porcine FGF23 ELISA kit is a quantitative sandwich enzyme-linked immunosorbent assay designed for the measurement of porcine fibroblast growth factor 23 (FGF23) levels in plasma or serum samples.

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Lab products found in correlation

N mid osteocalcin elisa, by Immunodiagnostic Systems (2 mentions) Intact pth elisa, by Immunodiagnostic Systems (1 mentions) Calcitonin ultrasensitive elisa, by DRG International (1 mentions) Porcine vd3 elisa kit, by Kendall Scientific (1 mentions) 25 hydroxy vitamin ds eia, by Immunodiagnostic Systems (1 mentions)

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Product FAQ

How does the Porcine FGF23 ELISA Kit by Wuhan Fine Biotech stand out in the research market?

Wuhan Fine Biotech's Porcine FGF23 ELISA Kit offers superior sensitivity and specificity compared to alternative products. While competitors like Immunodiagnostic Systems and DRG International produce similar immunoassay kits, this kit provides exceptional precision for bone and mineral metabolism research with optimized reagent formulations and stringent quality control.

What specific identification details should researchers use when referencing this ELISA kit?

What laboratory systems and research contexts are compatible with this FGF23 ELISA kit?

What primary scientific research domains utilize this Porcine FGF23 ELISA Kit?

What fascinating scientific insight relates to FGF23 research?

3 protocols using «porcine fgf23 elisa kit»

Plasma Vitamin D3 and FGF-23 Measurement

2022

Plasma vitamin D₃ (VD₃; Catalog no. PV0037, Porcine VD₃ ELISA Kit, Kendall Scientific, IL, USA) and serum FGF-23 (Catalog no. EP0059, Porcine FGF-23 ELISA Kit, Wuhan Fine Biotech Co., Ltd., Wuhan, China) concentrations were measured according to manufacturers’ protocols. Using Epoch microplate spectrophotometer (BioTek® Instruments, Inc. Highland Park, VT, USA), the VD₃ and FGF-23 absorbance were detected at 450 nm. The intra-assay coefficient of variation for VD₃ and FGF-23 were 3.97% and 1.52%, respectively.

Habibi M., Shili C.N., Sutton J., Goodarzi P, & Pezeshki A. (2022). Dietary branched-chain amino acids modulate the dynamics of calcium absorption and reabsorption in protein-restricted pigs. Journal of Animal Science and Biotechnology, 13, 15.

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Analytical Methods for Serum Biochemistry

2021

An auto analyzer for clinical chemistry using enzymatic colorimetric assays (Cobas 6000/c501; Roche Diagnostics GmbH, Rotkirch, Canton of Zug, Switzerland) was used to determine serum content of P, Ca, ALP, cholesterol, nonesterified fatty acids (NEFA), triglycerides, and urea. The Ca:P ratio in serum was calculated to assess the systemic availability of Ca and P and their association with the regulatory hormones (Veum, 2010 ; Gerlinger et al., 2020 (link); Vötterl et al., 2020 (link)). A porcine-specific enzyme-linked immunosorbent assay (ELISA) was used to determine FGF23 (Porcine FGF23 ELISA kit, Wuhan Fine Biotech Co., Ltd., Wuhan, Hubei, China; coefficient of variation [CV] < 10%). For calcitonin, a porcine-specific ELISA kit (Calcitonin ultrasensitive ELISA; DRG Instruments GmbH, Marburg, Hesse, Germany; CV < 8%) was used and the non-diluted samples were analyzed. Nevertheless, most of the samples contained calcitonin levels at the quantification limit of 0.7 pg/mL and were, therefore, not reported. Vitamin D (25-Hydroxy Vitamin Ds EIA, Immunodiagnostic Systems Holdings PLC, Tyne & Wear, NE35 9PD, UK; CV < 20%) and OCN (N-MID Osteocalcin ELISA, Immunodiagnostic Systems Holdings PLC, Tyne & Wear, NE35 9PD, UK; CV < 6%) were analyzed with commercial ELISA kits that were previously evaluated for pig serum (Kolp et al., 2017 (link); Sørensen et al., 2018 (link)).

Vötterl J.C., Klinsoda J., Hennig-Pauka I., Verhovsek D, & Metzler-Zebeli B.U. (2021). Evaluation of serum parameters to predict the dietary intake of calcium and available phosphorus in growing pigs. Translational Animal Science, 5(2), txab059.

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Porcine Serum Mineral and Hormone Evaluation

Cited 2 times 2020

The serum content of total P, total Ca, and alkaline phosphatase (ALP) were determined with enzymatic colorimetric assays using an autoanalyzer for clinical chemistry (Cobas 6000/c501; Roche Diagnostics GmbH, Rotkreuz, Switzerland). Commercial porcine-specific ELISA kits were used to determine FGF23 (Porcine FGF23 ELISA kit; Wuhan Fine Biotech Co., Ltd., Hubei, China). Calcitonin was determined using a porcine-specific ELISA kit (Calcitonin ultrasensitive ELISA; DRG Instruments GmbH, Marburg, Germany); however, most samples contained levels below the quantification limit of 0.7 pg/mL. VitD (25-Hydroxy Vitamin D EIA; Immunodiagnostic Systems Holdings PLC, Tyne & Wear, United Kingdom), OCN (N-MID Osteocalcin ELISA; Immunodiagnostic Systems Holdings PLC, Tyne & Wear, United Kingdom), and PTH (Intact PTH ELISA; Immunodiagnostic Systems Holdings PLC, Tyne & Wear, United Kingdom) were analyzed with commercial ELISA kits for humans that were used in previous pig studies [54 (link),55 (link)].

Vötterl J.C., Klinsoda J., Zebeli Q., Hennig-Pauka I., Kandler W, & Metzler-Zebeli B.U. (2020). Dietary Phytase and Lactic Acid-Treated Cereal Grains Differently Affected Calcium and Phosphorus Homeostasis from Intestinal Uptake to Systemic Metabolism in a Pig Model. Nutrients, 12(5), 1542.

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