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Magnetic stirrer

Manufactured by As One
Sourced in Japan

A magnetic stirrer is a laboratory equipment used to create a uniform mixture by continuously stirring a liquid sample. It consists of a rotating magnetic field that drives a small magnetic bar immersed in the liquid, causing it to spin and mix the contents.

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8 protocols using magnetic stirrer

1

Preparation of PLGA Copolymer Solution

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Example 2

<Preparation of Solution B>

Polylactic acid-glycolic acid copolymer (product name: PLGA-5010, obtained from Wako Pure Chemical Industries, Ltd.) (4 parts by mass), acetone (obtained from Wako Pure Chemical Industries, Ltd.) (76.8 parts by mass), and methanol (obtained from Wako Pure Chemical Industries, Ltd.) (19.2 parts by mass) were mixed under stirring using a stirrer (device name: magnetic stirrer, obtained from AS ONE Corporation) for 1 hour at 1,000 rpm, followed by passing the resultant through a 1 μm-filtration filter (product name: Millex SLFA05010, obtained from Merck), to prepare solution B.

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2

Preparation of PLGA-Based Solution F

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Example 6

<Preparation of Solution F>

Polylactic acid-glycolic acid copolymer (product name: PLGA-5010, obtained from Wako Pure Chemical Industries, Ltd.) (4 parts by mass), acetone (obtained from Wako Pure Chemical Industries, Ltd.) (57.6 parts by mass), and ethanol (obtained from Wako Pure Chemical Industries, Ltd.) (38.4 parts by mass) were mixed under stirring using a stirrer (device name: magnetic stirrer, obtained from AS ONE Corporation) for 1 hour at 1,000 rpm, followed by passing the resultant through a 1 μm-filtration filter (product name: Millex SLFA05010, obtained from Merck), to prepare solution F.

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3

Preparation of PLGA-based Solution C

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Example 3

<Preparation of Solution C>

Polylactic acid-glycolic acid copolymer (product name: PLGA-5010, obtained from Wako Pure Chemical Industries, Ltd.) (4 parts by mass), acetone (obtained from Wako Pure Chemical Industries, Ltd.) (86.4 parts by mass), and methanol (obtained from Wako Pure Chemical Industries, Ltd.) (9.6 parts by mass) were mixed under stirring using a stirrer (device name: magnetic stirrer, obtained from AS ONE Corporation) for 1 hour at 1,000 rpm, followed by passing the resultant through a 1 μm-filtration filter (product name: Millex SLFA05010, obtained from Merck), to prepare solution C.

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4

Preparation of PLGA-based Solution E

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Example 5

<Preparation of Solution E>

Polylactic acid-glycolic acid copolymer (product name: PLGA-5010, obtained from Wako Pure Chemical Industries, Ltd.) (4 parts by mass), methyl ethyl ketone (obtained from Wako Pure Chemical Industries, Ltd.) (67.2 parts by mass), and methanol (obtained from Wako Pure Chemical Industries, Ltd.) (28.8 parts by mass) were mixed under stirring using a stirrer (device name: magnetic stirrer, obtained from AS ONE Corporation) for 1 hour at 1,000 rpm, followed by passing the resultant through a 1 μm-filtration filter (product name: Millex SLFA05010, obtained from Merck), to prepare solution E.

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5

Preparation of PLGA-based Polymer Solution

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Example 4

<Preparation of Solution D>

Polylactic acid-glycolic acid copolymer (product name: PLGA-5010, obtained from Wako Pure Chemical Industries, Ltd.) (4 parts by mass), ethyl acetate (obtained from Wako Pure Chemical Industries, Ltd.) (67.2 parts by mass), and methanol (obtained from Wako Pure Chemical Industries, Ltd.) (28.8 parts by mass) were mixed under stirring using a stirrer (device name: magnetic stirrer, obtained from AS ONE Corporation) for 1 hour at 1,000 rpm, followed by passing the resultant through a 1 μm-filtration filter (product name: Millex SLFA05010, obtained from Merck), to prepare solution D.

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6

Preparation of PLGA-5010 Solution

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Example 7

<Preparation of Solution G>

Polylactic acid-glycolic acid copolymer (product name: PLGA-5010, obtained from Wako Pure Chemical Industries, Ltd.) (4 parts by mass) and acetone (obtained from Wako Pure Chemical Industries, Ltd.) (96 parts by mass) were mixed under stirring using a stirrer (device name: magnetic stirrer, obtained from AS ONE Corporation) for 1 hour at 1,000 rpm, followed by passing the resultant through a 1 μm-filtration filter (product name: Millex SLFA05010, obtained from Merck), to prepare solution G.

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7

PG Vapor Inactivation of IAV

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Three cotton balls (Yamatokojo Co., Ltd., Osaka, Japan) soaked in a total of 1 mL PG were placed at the bottom of a 1 L glass bottle (Tokyo Garasu Kikai Co., Ltd., Tokyo, Japan) and stirred at 500 rpm using a magnetic stirrer (As One Corporation, Osaka, Japan) at 22–23 °C for 30 min. IAV solution (1.5 μL; 8.3 × 105 FFU) was dried on the lid of a cryo vial (Thermo Fisher Scientific Japan K.K.) for 30 min at 22–23 °C. This lid was attached to the inside of a 1 L bottle cap. The cap was then replaced on the 1 L bottle with the IAV lid attached, exposing the lid to PG vapor for 30 min at 22–23 °C. After exposure, the lid was removed from the cap. The IAV on the lid was dissolved in SFM and immediately titrated using the focus-forming assay described below.
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8

Preparation of PLGA-based Solution H

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Example 8

<Preparation of Solution H>

Polylactic acid-glycolic acid copolymer (product name: PLGA-5010, obtained from Wako Pure Chemical Industries, Ltd.) (4 parts by mass) and methyl acetate (obtained from Wako Pure Chemical Industries, Ltd.) (96 parts by mass) were mixed under stirring using a stirrer (device name: magnetic stirrer, obtained from AS ONE Corporation) for 1 hour at 1,000 rpm, followed by passing the resultant through a 1 μm-filtration filter (product name: Millex SLFA05010, obtained from Merck), to prepare solution H.

Next, each of the prepared solutions A to H was charged into a solution housing container 13 made of glass presented in FIG. 2.

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