Analytical grade (to enable its use without further purification) rhodamine 123 (R123); sodium dodecyl sulfate (SDS); 3-(4.5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide (MTT); Dulbecco’s Modified Eagle’s medium–high glucose (DMEM) with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin mixture; resazurin sodium salt; trypsin-EDTA solution; allantoin; and tariquidar and dimethyl sulfoxide (DMSO) were acquired at Sigma-Aldrich (St. Louis, MO, USA). Doxorubicin hydrochloride was acquired from Teva Pharmaceuticals (Petah Tikva, Israel). Eagle’s Minimal Essential Medium (EMEM, Sigma-Aldrich) containing 4500 mg/L glucose, supplemented with a non-essential amino acid (NEAA) mixture (Sigma-Aldrich); a selection of vitamins and 10% heat-inactivated FBS; 2 mM L-glutamine (Sigma-Aldrich); 1 mM Na-pyruvate (Sigma-Aldrich); nystatin (Sigma-Aldrich); a penicillin-streptomycin mixture at concentrations of 100 U/L and 10 mg/L; RPMI 1640 medium (Sigma-Aldrich), supplemented with 10% FBS; 2 mM L-glutamine; 1 mM Na-pyruvate; 100 mM HEPES (Sigma-Aldrich); nystatin; and a penicillin-streptomycin mixture at concentrations of 100 U/L and 10 mg/L were used in the biological evaluation. Pgp-Glo™ Assay Systems (Promega), and an Annexin V-FITC Apoptosis Detection Kit were used (Calbiochem, EMD Biosciences. Inc. La Jolla, CA, USA).
Na pyruvate
Manufactured by Merck Group
Sourced in United States, Germany, Sweden, Switzerland
Na-pyruvate is a laboratory reagent that serves as a source of the pyruvate anion. Pyruvate is a key metabolic intermediate in various biological processes, including glycolysis and the citric acid cycle. Na-pyruvate provides a convenient and stable form of pyruvate for use in cell culture media, enzymatic assays, and other research applications.
Automatically generated - may contain errors
Lab products found in correlation
L glutamine, by Merck Group (16 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (9 mentions)
Non essential amino acids (neaa), by Merck Group (8 mentions)
Penicillin, by Merck Group (8 mentions)
Streptomycin, by Merck Group (8 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (8 mentions)
Hepes, by Merck Group (7 mentions)
Nah2po4, by Merck Group (7 mentions)
Nahco3, by Merck Group (7 mentions)
Sodium chloride (nacl), by Merck Group (7 mentions)
Penicillin streptomycin, by Merck Group (6 mentions)
Non essential amino acids (neaa), by Thermo Fisher Scientific (5 mentions)
Insulin, by Merck Group (4 mentions)
Na ascorbate, by Merck Group (4 mentions)
Potassium chloride (kcl), by Merck Group (4 mentions)
Bovine serum albumin (bsa), by Merck Group (4 mentions)
Tween 20, by Merck Group (4 mentions)
Glutamax, by Thermo Fisher Scientific (4 mentions)
Ethylenediaminetetraacetic acid, by Merck Group (3 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (3 mentions)
66 protocols using na pyruvate
1
Rhodamine 123 Cell Viability and Apoptosis Assay
2
Ion Transport Modulation Assay
3
Intestinal Tissue Preparation and Ussing Chamber Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Experiments were performed as previously described [48 (link)]. Briefly, dissected tissue specimens were flushed with cold KREB’s buffer to remove fecal material, kept on ice in KREB’s buffer for 30 min, opened along the mesenteric border and mounted in the Ussing chambers (exposed area 0.25 cm2). Both the serosal and mucosal chambers were filled with 2 ml KREB’s buffer and gassed with 95% O2 and 5% CO2 at 37 ºC, pH 7.4. The serosal solution also contained 5.1 mM Na-L-Glutamate, 5.7 mM Na-Pyruvate and 10 mM D-Glucose (Riedel-Haen AG) and the mucosal solution contained 5.13 mM Na-L-Glutamate (Merck), 5.7 mM Na-Pyruvate (Sigma-Aldrich) and 10 mM D-Mannitol (BDH Laboratory supplies).
4
Chondrogenic Induction of Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Two-dimensional chondrogenic induction was performed as previously described [32 (link)]. Briefly, cells (1.5 x105) were suspended in 5 μl of chondrogenic medium (DMEM/F12 (Life Technologies), 1% (v/v) ITS1 mix (BD), 0.17 mM AA2P (Sigma), 0.35 mM Proline (Sigma), 0.1 μM dexamethasone (WAKO), 0.15% (v/v) glucose (Sigma), 1 mM Na-pyruvate (Sigma), and 2 mM GlutaMax (Life Technologies) supplemented with 40 ng/ml PDGF-BB (R&D System) and 1% (v/v) FBS (Nichirei, Inc., Tokyo, Japan)). They were subsequently transferred to fibronectin-coated 24-well plates (Corning, Inc., NY, USA). One milliliter of the chondrogenic medium was added after 1 h. TGFb3 (R&D System) was subsequently added at 10 ng/ml on days 6 to 10. Differentiation was confirmed on day 10 using Alcian Blue staining.
5
Measuring Oxygen Consumption in Mouse Tissues
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Oxygen consumption was assessed in fresh tissues from mice after CO2 asphyxiation; tissues were immediately placed into warm (37 °C, DMEM (4.5 g/l glucose, l -glutamine, Wisent, St. Bruno, QC)) media. Tissues were weighed (∼10–20 mg), washed in filtered warm 37 °C respiration buffer (PBS, 0.02% fatty acid free BSA, 25 μM glucose, 0.01% (vol/vol) of 100 mM Na pyruvate (Sigma)), minced with dissection scissors, re-suspended in 1 ml respiration buffer and placed into a Mitocell chamber (MT200A, Strathkelvin Instruments, North Lanarkshire, Scotland) with a Clark electrode (Strathkelvin). For each analyzed tissue, measurements were obtained from three pooled groups of fragments of equivalent size (∼10–20 mg) and normalized to tissue weight.
6
Culturing JIMT-1 Breast Cancer and Human Dermal Fibroblasts
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human breast carcinoma cell line JIMT-1 was purchased from the German Collection of Microorganisms and Cell Cultures (Braunschweig, Germany). The cells were tested for mycoplasma during the experimental period and were found to be negative (Eurofins Scientific, Cologne, Germany). HDFs were purchased from Sigma-Aldrich Sweden AB (Stockholm, Sweden). The HDFs were maximally used for up to 7 passages. The cell lines were cultured in Dulbecco’s modified Eagle medium/Ham’s F-12 (1:1) supplemented with 5 % heat-inactivated donor herd horse serum (DHHS) (Sigma-Aldrich Sweden AB), 5 μg/mL insulin (Sigma-Aldrich Sweden AB), 10 ng/mL epidermal growth factor (Lonza, Basel, Switzerland), 0.5 μg/mL hydrocortisone (Sigma-Aldrich Sweden AB), 1 mM Na-pyruvate (Sigma-Aldrich Sweden AB), 50 μg/mL transferrin (Sigma-Aldrich Sweden AB), 2 mM l -glutamine (VWR, Lund, Sweden), 1 mM non-essential amino acids (VWR), 100 μg/mL streptomycin (VWR), and 100 U/mL penicillin (VWR). The cell lines were maintained in a humidified incubator (95 % humidity) with 5 % CO2 in air at 37 °C (CO2 incubator). The cells were passaged twice a week using Accutase™ (Sigma-Aldrich Sweden AB).
7
Culturing and Maintaining JIMT-1 and HDF Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human breast carcinoma cell line JIMT-1 (ACC-589) was purchased from the German Collection of Microorganisms and Cell Cultures (Braunschweig, Germany). The cells were tested for mycoplasma during the experimental period and were found to be negative (Eurofins Scientific, Cologne, Germany). HDFs (106-05a) were purchased from Sigma-Aldrich Sweden AB (Stockholm, Sweden). The HDFs were maximally used between passages 3–6. The cell lines were cultured in Dulbecco’s modified Eagle medium/Ham´s F-12 (1:1) supplemented with 5% heat-inactivated donor herd horse serum (DHHS) (Sigma-Aldrich Sweden AB), 5 μg/ml insulin (Sigma-Aldrich Sweden AB), 10 ng/ml epidermal growth factor (Lonza, Basel, Switzerland), 0.5 µg/ml hydrocortisone (Sigma-Aldrich Sweden AB), 1 mM Na-pyruvate (Sigma-Aldrich Sweden AB), 50 µg/ml transferrin (Sigma-Aldrich Sweden AB), 2 mM L-glutamine (VWR, Lund, Sweden), 1 mM non-essential amino acids (VWR), 100 μg/ml streptomycin (VWR), and 100 U/ml penicillin (VWR). The cell lines were maintained in a humidified incubator (95% humidity) with 5% CO2 in air at 37 ºC (CO2 incubator). The cells were passaged twice a week using Accutase (Sigma-Aldrich Sweden AB).
8
Neural Progenitor Cell Proliferation Medium
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Example 3
Proliferation medium was prepared with the following components in the indicated concentrations: Component Final Concentration DMEM, glutamine (Invitrogen, cat#25030-081) 2 mM, Na Pyruvate (Sigma, cat# S8636), 1 mM, NAC (Sigma, cat# A9165), 1 mM, N2 supplement (Invitrogen, cat#17502-048; containing transferrin, insulin, putrescine, selenium and progesterone), B27 supplement (Invitrogen, cat #17504-044), 20 ng/ml human bFGF (Biosource, cat# PHG0024), 20 ng/ml PDGF-AA (Peprotech, cat#100-13A) 10 ng/ml NT3 (Peprotech, cat#450-03), 100 ng/ml IGF1 (Peprotech, cat #AF-100-11).
9
Cultivation of BeWo and HBMEC Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The placental choriocarcinoma cell line BeWo (ATCC® CCL-98™, passages 195 to 207) was cultured in F-12K Medium (ATCC® 30-2004) supplemented with 10% FBS (Gibco, Thermo Fisher Scientific). Human Brain Microvascular Endothelial Cells (HBMEC), passages 3 to 20, were cultured in RPMI-1640 medium with L-glutamine (Gibco, Thermo Fisher Scientific) supplemented with 1mM Na-Pyruvate (Sigma-Aldrich), 1% MEM non-essential amino acids (Sigma-Aldrich), 1% MEM Vitamin Solution x100 (Sigma-Aldrich), 10% Nu-Serum (Corning®, VWR, Switzerland) and 10% FBS (Gibco, Thermo Fisher Scientific), adapted from Silwedel et al. (2018) (link). Both cell lines were cultivated in 75 cm2 culture flasks (Bioswisstech, Switzerland) at 37°C and 5% CO2 in a humid atmosphere. To maintain the culture monolayer cell cultures were split when 70 to 90% confluence was reached.
10
Autophagy Regulation in Mouse Models
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
C57BL/6 (wild-type [WT]) mice were purchased from Jackson Labs (Bar Harbor, ME) and then bred in the Cedars-Sinai Medical Center Comparative Medicine facility. Atg16l1fl/flLysmcre mice were obtained from Dr. David Shih, Cedars-Sinai medical Center (26 (link)). Atg16l1fl/fl mice were used as littermate controls. Eight- to ten-weeks-old female mice were used in all experiments in this study. WT, Atg16l1+/−, and Atg5−/− mouse embryonic fibroblasts (MEF)s were obtained from Dr. Jae Jung (University of Southern California) (27 (link)) and grown in DMEM media containing 10% FBS. In some cases, 1 μg/ml cycloheximide (Sigma Aldrich, St. Louis MO), MEM amino acids (Sigma Aldrich, St. Louis MO), or Na-pyruvate (Sigma Aldrich, St. Louis MO) were added to the media. Atg5fl/flLysmcre and Atg5fl/fl bone marrow was provided by Dr. Herbert W. Virgin (Washington University, Saint Louis).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!