CO2 incubator
The CO2 incubator is a laboratory equipment designed to provide a controlled environment for cell and tissue culture applications. It maintains a consistent temperature, humidity, and carbon dioxide (CO2) concentration to support the optimal growth and maintenance of cultured cells.
Lab products found in correlation
5 protocols using CO2 incubator
Fibroblast Response to Hanwoo Infusions
Evaluating Biocompatibility of Drug-Loaded Nanoparticles
Synergistic Osteogenesis of hAD-MSCs
Primary hAD-MSCs were used to investigate the influence of HPGs as scaffolding building blocks on the behavior of the cell. HPGs (2 mg) were placed in each well of a 24-well plate and sterilized using 70% (v/v) ethanol solution for 1 h, then dried and washed twice in PBS for 30 minutes. The cells were seeded at a density of 3500 cells per well and incubated with the basal medium of DMEM/F12 containing l-glutamine/10% FBS/1% antibiotics at 37°C in a humidified atmosphere of 5% CO2 incubator (ASTEC, Fukuoka, Japan). The medium was replaced every 48 hours.
To investigate the synergistic differentiation behavior, two test groups of HPGs and HPGs-BMP4 were used. Osteogenic differentiation culture medium was constituted of the basal medium supplemented with dexamethasone (10 nM), β-glycerophosphate (10 mM), and ascorbic acid (50 μM).
Cytotoxicity Assay of CβG/PLGA Nanoparticles
Skin Fibroblast Cell Proliferation Assay
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