H1 hesc line

Q: What makes h1 hesc line unique in stem cell research?

h1 hesc line offers exceptional characteristics in human embryonic stem cell research, developed by WiCell with rigorous quality standards. While alternative products exist from providers like Thermo Fisher Scientific and STEMCELL, this line provides superior genetic stability and reproducibility for complex stem cell studies.

Q: What citation details are critical when referencing h1 hesc line?

When citing h1 hesc line, include the WiCell catalog number, specific cell line designation, passage number, and original publication source. Proper academic citation should reference WiCell as the originating institution and include complete molecular characterization details.

Q: What laboratory systems are compatible with h1 hesc line?

h1 hesc line demonstrates excellent compatibility with essential stem cell culture systems, including essential 8 medium, matrigel, rhlaminin 521, knockout serum replacement, and supporting reagents like accutase and y-27632. Optimal performance is achieved with carefully selected complementary products.

Q: What research domains primarily utilize h1 hesc line?

h1 hesc line is predominantly utilized in regenerative medicine, developmental biology, disease modeling, drug screening, and advanced cellular differentiation research. Its applications span neuroscience, cardiovascular studies, and personalized medicine investigations.

Q: What innovative breakthrough is associated with h1 hesc line?

The h1 hesc line played a pivotal role in demonstrating the pluripotency and genetic stability of human embryonic stem cells, contributing significantly to understanding cellular reprogramming mechanisms and opening new frontiers in stem cell research.

Manufactured by WiCell

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About the product

The H1 hESC line is a well-characterized human embryonic stem cell line derived from a donated blastocyst. It is widely used in research as a model system for studying human embryonic development, cell differentiation, and other biological processes.

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Spelling variants (same manufacturer)

H1 hESCs - 49 citations HESC lines H1 and H9 - 16 citations H1 line - 4 citations H1 (WA01) hESC line - 4 citations HESC lines H1 - 3 citations POU5F1-eGFP H1 hESC line - 3 citations HESCs (H1, - 2 citations HESC lines - 2 citations HESC H1 and H9-EGFP lines - 2 citations

The spelling variants listed below correspond to different ways the product may be referred to in scientific literature.
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Product FAQ

What makes h1 hesc line unique in stem cell research?

What citation details are critical when referencing h1 hesc line?

What laboratory systems are compatible with h1 hesc line?

What research domains primarily utilize h1 hesc line?

What innovative breakthrough is associated with h1 hesc line?

33 protocols using «h1 hesc line»

Differentiation of hESC-Derived Islet-Like Cells

2025

The H1 hESC line (WiCell, Madison, WI, USA) was cultured and expanded
using StemMACSiPSC-Brew XF media (Miltenyi Biotec) on tissue culture
plates coated with Geltrex LDEV-free hESC-qualified basement membrane
matrix (Gibco). The differentiation protocol began 48 h after seeding
4–4.5 × 10⁶ cells per 12-well tissue culture-treated
plate (Corning) and when cells were 60–90% confluent. hESC-derived
islet-like cell clusters (hESC-islet) were generated with a modified
version of a previously unpublished 7-stage differentiation protocol
(Misra et al., unpublished). Stage 7 hESC-islets were analyzed by
using flow cytometry for their efficient differentiation to C-peptide⁺/NKX6.1⁺ monohormonal β-like cells. HESC
use was approved by the Stem Cell Oversight Committee (Canadian Institute
of Health Research).

Wang Y., Gulati N., Regeenes R., Migliorini A., Oakie A., Nostro M.C, & Rocheleau J.V. (2025). Modulating the Kinetics of a Fluorescence Anisotropy Immunoassay Using Tracer Point Mutations to Measure Human C-Peptide Secretion On-Chip. ACS Omega, 10(11), 11595-11606.

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Culturing H1 hESC in E8 Medium

2025

The H1 hESC line was purchased from WiCell Institute and cultured in Essential 8 (E8) medium (Thermo Fisher, Cat# A1517001) in rhLaminin-521 (Thermo Fisher, Cat# A29249) coated wells with medium change daily. Cells were maintained at 37 °C in a humidified incubator with sea-level air enriched with 5% CO₂.

Bi C., Yuan B., Zhang Y., Wang M., Tian Y, & Li M. (2025). Prevalent integration of genomic repetitive and regulatory elements and donor sequences at CRISPR-Cas9-induced breaks. Communications Biology, 8, 94.

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Generation and Characterization of hPSC Lines

2024

The H1 hESC line was purchased from WiCell Institute. The H1-iCas9 ESC line is a gift from Danwei Huangfu’s laboratory. The wild-type iPSC line was reprogrammed and well characterized in previous studies [44 (link), 56 (link), 57 (link)]. The study was approved by the KAUST Institutional Biosafety and Bioethics Committee (IBEC). All hPSCs were cultured in Essential 8 medium (ThermoFisher, Cat# A1517001) in rhLaminin-521 (ThermoFisher, Cat# A29249) coated wells with medium change daily. The peripheral blood mononuclear cells were isolated from the whole blood of a healthy donor via a standard Ficoll-Paque-based protocol and further cultured in StemSpan™-ACF Erythroid Expansion medium (STEMCELL Technology, Cat# 09860) for 13 days with medium change every 3 days to expand the erythroid progenitors. The erythroid progenitors were analyzed by FACS before CRISPR-Cas9 editing.

Yuan B., Bi C., Tian Y., Wang J., Jin Y., Alsayegh K., Tehseen M., Yi G., Zhou X., Shao Y., Romero F.V., Fischle W., Izpisua Belmonte J.C., Hamdan S., Huang Y, & Li M. (2024). Modulation of the microhomology-mediated end joining pathway suppresses large deletions and enhances homology-directed repair following CRISPR-Cas9-induced DNA breaks. BMC Biology, 22, 101.

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Cardiomyocyte Differentiation from Human Embryonic Stem Cells

2024

Human embryonic stem cell (hESC) H1 line was obtained from WiCell Research and maintained on feeder-free plates pre-coated with Matrigel (BD Biosciences) using mTeSRTM1 media (STEMCELL Technologies Inc.). The cardiomyocyte differentiation was derived from hESCs. In short, dissociated hESCs were seeded on Matrigel pre-coated dishes in mTeSR media in the presence of ROCK inhibitor Y-27632 (Reagents Direct,Cat. 53-B85-50). A GSK inhibitor, CHIR (Selleck, Cat. S2924) was applied for 24 h, followed by addition of the canonical Wnt-signaling inhibitor, IWP4 (Stemgent, Cat. 04–0036). During differentiation, cells were cultured in RPMI medium plus B-27 Supplement.

Hernandez-Benitez R., Wang C., Shi L., Ouchi Y., Zhong C., Hishida T., Liao H.K., Magill E.A., Memczak S., Soligalla R.D., Fresia C., Hatanaka F., Lamas V., Guillen I., Sahu S., Yamamoto M., Shao Y., Aguirre-Vazquez A., Nuñez Delicado E., Guillen P., Rodriguez Esteban C., Qu J., Reddy P., Horvath S., Liu G.H., Magistretti P, & Izpisua Belmonte J.C. (2024). Intervention with metabolites emulating endogenous cell transitions accelerates muscle regeneration in young and aged mice. Cell Reports Medicine, 5(3), 101449.

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Authentication and Characterization of iPSC Lines

2024

All experiments involving human cells were performed according to the International Society for Stem Cell Research (ISSCR) 2016 guidelines and approved by the Harvard University Institutional Review Board and Embryonic Stem Cell Research Oversight (ESCRO) committees. The 11a iPSC line (RRID: CVCL_8987) was obtained from the Harvard Stem Cell Institute. The PGP1 iPSC line was derived from the laboratory of G. Church (Church, G.M., 2005; RRID: CVCL_F182). The H1 hESC line (also known as WA01; RRID: CVCL_9771) was purchased from WiCell. The cells were cultured on Geltrex-coated dishes (Gibco) in mTeSR1 medium (Stem Cell Technologies) supplemented with 100 U/mL penicillin and 100 μg/mL streptomycin and maintained at 37°C in 5% CO₂. Cell cultures were routinely tested and found negative for Mycoplasma and were karyotypically normal. PGP1 and H1 cell lines were authenticated using short tandem repeat analysis completed by TRIPath (2018) and WiCell (2021), respectively. For authentication of the 11a cell line, refer to Quadrato and colleagues (49 (link)).

Mangena V., Chanoch-Myers R., Sartore R., Paulsen B., Gritsch S., Weisman H., Hara T., Breakefield X.O., Breyne K., Regev A., Chung K., Arlotta P., Tirosh I, & Suvà M.L. (2024). Glioblastoma Cortical Organoids Recapitulate Cell-State Heterogeneity and Intercellular Transfer. Cancer Discovery, 15(2), 299-315.

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