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Anti-Flavivirus group antigen antibody (4G2)

Manufactured by Absolute Antibody
Sourced in United Kingdom

The Anti-Flavivirus group antigen antibody (4G2) is a laboratory reagent used for the detection and identification of flavivirus group antigens. It is a monoclonal antibody that recognizes a conserved epitope on the envelope (E) protein of various flaviviruses, including dengue virus, yellow fever virus, and West Nile virus. This antibody can be used in various immunoassays and research applications to study the presence and distribution of flavivirus antigens.

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2 protocols using Anti-Flavivirus group antigen antibody (4G2)

Confluent monolayers of A549 cells in 12-well plates were inoculated with TBEV for 2 h at 37 °C. After the removal of the virus, the cells were washed and overlaid with carboxymethylcellulose in D-MEM medium with DMSO or different concentrations of compounds. Two days post-infection, cells were washed with phosphate-buffered saline (PBS), fixed with methanol, and infected foci were visualized by immunostaining with the monoclonal anti-Flavivirus group antigen antibody (4G2) (Absolute Antibody, Oxford, UK; diluted 1:1500 in PBS, 1% Tween 20, 5% FBS), followed with anti-mouse horseradish peroxidase (HRP)-conjugated secondary antibody (diluted 1:2000 in PBS containing 1% Tween 20 and 5% FBS). Plaques were detected using a Vector Nova Red kit (Vector Laboratories Ltd., Peterborough, UK) and counted.
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Monolayers of A549 cells seeded in 12-well plates were inoculated with TBEV at MOI of 0.001. Virus inoculum was removed after 2 h at 37 °C, the cells were washed and overlaid with 1% carboxymethylcellulose in Modified Eagle’s Medium (MEM) medium with DMSO or tested compounds. After three days, cells were washed with phosphate-buffered saline (PBS) and fixed with methanol. The infected virus foci were visualized by immunostaining with the monoclonal anti-Flavivirus group antigen antibody (4G2) (Absolute Antibody, Oxford, UK; diluted 1:1500 in PBS, 0,05% Tween 80, 5% FBS) as a primary antibody and anti-mouse secondary antibody conjugated with horseradish peroxidase (HRP) (diluted 1:2000) in PBS containing 0,05% Tween 80 and 5% FBS. Plaques were detected using a Vector Nova Red kit (Vector Laboratories Ltd., Peterborough, UK) according to the manufacturer’s protocol and counted. If necessary, one-way ANOVA with Dunnett’s post-test was performed in some experiments for statistical analysis using the GraphPad Prism software (version 5.01, GraphPad Software, San Diego, CA, USA).
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