Pkh26

Manufactured by Merck Group

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About the product

PKH26 is a fluorescent cell linker that binds to the lipid bilayers of cell membranes. It is used for labeling and tracking cells in various biological applications.

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Lab products found in correlation

Pkh67, by Merck Group (142 mentions) Carboxyfluorescein succinimidyl ester (cfse), by Thermo Fisher Scientific (60 mentions) Diluent c, by Merck Group (34 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (29 mentions) Carboxyfluorescein succinimidyl ester (cfse), by Merck Group (25 mentions) Pkh26, by BD (24 mentions) Pkh26, by Thermo Fisher Scientific (23 mentions) 4 6 diamidino 2 phenylindole (dapi), by Beyotime (17 mentions) Bovine serum albumin (bsa), by Merck Group (17 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (17 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (15 mentions) 4 6 diamidino 2 phenylindole (dapi), by Solarbio (14 mentions) Pkh26, by Olympus (13 mentions) Matrigel, by Corning (13 mentions) Actin tracker green, by Beyotime (12 mentions) Fluorescence microscope, by Leica (11 mentions) Pkh26, by Beckman Coulter (11 mentions) Matrigel, by BD (11 mentions) Dimethyl sulfoxide (dmso), by Merck Group (11 mentions) Rpmi 1640, by Thermo Fisher Scientific (11 mentions)

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Market Availability & Pricing

The PKH26 Cell Membrane Labeling Kit is an active product offered by Merck Group. It is available through authorized distributors. Pricing for the kit typically ranges from $235.00 to $1,228.57, depending on the kit size and distributor.

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Spelling variants (same manufacturer)

PKH26 Red Fluorescent Linker Kit - 9 citations PKH26-labeled - 5 citations PKH26 MINI kit - 4 citations PKH26/67 - 3 citations PKH26 0.5 μM dye - 2 citations PKH26 staining solution - 2 citations

Similar products (other manufacturers)

PKH26 (by Thermo Fisher Scientific) - 62 citations PKH26 (by Umibio) - 25 citations PKH26 (by Olympus) - 13 citations PKH26 (by Nikon) - 10 citations PKH26 (by MedChemExpress) - 6 citations PKH26 (by Tecan) - 5 citations PKH26 (by MKBio) - 3 citations PKH26 (by Corning) - 3 citations PKH26 (by Dojindo Laboratories) - 2 citations PKH26 (by GLPBIO) - 2 citations

The spelling variants listed below correspond to different ways the product may be referred to in scientific literature.
These variants have been automatically detected by our extraction engine, which groups similar formulations based on semantic similarity.

1 617 protocols using «pkh26»

Adipose-Derived Stem Cell Therapy for OA

2025

Under general anaesthesia and aseptic conditions, inguinal adipose tissue (AT) was surgically collected and processed, as described in detail in a previous article (Desando et al., 2021 (link)), for i) the generation of in vitro expanded ASC and ii) the production of mctSVF. For the expanded-ASC, inguinal AT was harvested 2 weeks before IA treatments and treated with 0.4 U/mL NB4 collagenase standard grade (Serva Electrophoresis, GmbH, Heidelberg, Germany, Cat. DS17454.01) for 30 min at 37°C to obtain the SVF and then kept a fortnight until passage 2 in α-MEM (Gibco, Carlsbad, CA, United States) medium containing 15% fetal bovine serum (FBS, Euroclone) and 0.05 g/mL penicillin G (Gibco). Cell count was evaluated at SVF isolation and expansion.
As for mctSVF, the inguinal AT was harvested on the same day of IA injection and processed in a single sterile disposable device Hy-Tissue SVF (Fidia Farmaceutici, Abano Terme, Italy). After collection, AT was minced with scissors, resuspended with saline, and then moved into a closed sterile bag to perform mechanical fragmentation. Duografter (Fidia Farmaceutici) subjected the filtered intermediate adipose product to centrifugation at 600 g for 10 min to generate the final product (Desando et al., 2021 (link)).
Eight weeks after OA induction, animals received IA injection of 2 × 10⁶ ASC (n = 8) or mctSVF (n = 8) in each knee to assess their efficacy. For local biodistribution groups, 2 ×10⁶ ASC and mctSVF were labelled with 2 μM PKH26 (Sigma-Aldrich, Cat. PKH26GL), and injected in each knee to monitor cell migration at 1 month from treatments. Live&Dead test (Thermo Fisher Scientific, Waltham, MA, United States, Cat. L10119) was performed on all adipose products for efficacy analyses before the IA treatment. Live (green staining) and dead (red staining) cells were evaluated with the DS-Ri2 microscope (Nikon, Tokyo, Japan), by using fluorescence channels: fluorescein isothiocyanate (FITC) and tetramethyl rhodamine isothiocyanate (TRITC). Trypan blue staining was used to assess the number of viable labelled cells in the cell suspension before IA injection for biodistribution analysis. The dye stains non-viable cells in blue, while it is excluded from living cells.

Desando G., Tschon M., Martini L., Sartori M., Giavaresi G., Fini M., Cellamare A., Soranzo C., Longinotti C., D’Alessandro M., Roseti L, & Grigolo B. (2025). 3D connective micro-fragment enriched with stromal vascular fraction in osteoarthritis: chondroprotective evidence in a preclinical in vivo model. Frontiers in Cell and Developmental Biology, 13, 1533405.

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Labeling Extracellular Vesicles with PKH26

2025

pEVs were labelled with the red fluorescent membrane dye PKH26 (λ_ex 551 nm; λ_em 567 nm; MIDI26, Sigma, MO, USA). Briefly, 100 µL of fresh pEVs and the PKH26 dye were gently mixed in 250 µL of dilution media for 10 min at room temperature. The reaction was stopped by adding ¼ (v/v) of exosomes‐depleted foetal bovine serum (Life Technologies Corporation, NY, USA) for 1 min. Excess of PKH26 was removed by washing in PBS 1X, filtered on a 100 kDa molecular weight filter (Millipore Sigma, MO, USA) and centrifuged at 3000 × g for 15 min at 4°C. The supernatants containing labelled pEVs were collected and kept overnight at 4°C before use. The analysis of PKH26‐labelled pEVs was performed with the violet side scattering (V‐SSC) using the 405 nm violet laser of the CytoFlex S (Beckman Coulter, CA, USA). The detection threshold for V‐SSC was set at 1800 with a gain of 200. Samples were run with a slow flow rate (10 µL/min) for 2 min until the event/second rate was stable and abort rates had decreased below 5%. The acquisition time was set at 30 s. Data were acquired and analysed using CytExpert 2.0 software (Beckman Coulter, CA, USA). Gated regions were determined to use unstained pEVs as controls. Relative diametres of pEVs were determined with standard submicron yellow‐green, fluorescent polystyrene beads with 0.2 and 0.5 µm sizes (Bangs Laboratories Inc. Fishers, IN, USA).

Counil H., Silva R.O., Rabanel J., Zaouter C., Haddad M., Ben Khedher M.R., Brambilla D., Fülöp T., Patten S.A, & Ramassamy C. (2025). Brain penetration of peripheral extracellular vesicles from Alzheimer's patients and induction of microglia activation. Journal of Extracellular Biology, 4(1), e70027.

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T Cell Proliferation Assay with Scaffold-Derived Conditioned Media

2025

Ethical approval was granted by the cantonal ethical committee of Grisons (BASEC-Nr. 2019–02353). The blood from 3 healthy donors was collected by venous puncture and transferred into potassium EDTA-coated tubes (S-Monovette®, Sarstedt). Peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll density gradient separation (Histopaque®-1077, Sigma-Aldrich).
PBMCs were kept in RPMI 1640 (Gibco) containing 2 mM L-glutamine, supplemented with 10 % (v/v) heat inactivated fetal calf serum (Biochrom), 1 % (v/v) penicillin-streptomycin (P/S, 100 U/mL penicillin and 100 μg/mL streptomycin, Gibco), 30 U/mL interleukin 2 (IL-2, PeproTech). Standard culture conditions were employed (37 °C, 5 % CO₂ and 95 % rH).
For the proliferation experiment, PBMCs were stained with 3 μM PKH26 (Sigma-Aldrich) and transferred to 48-well plates at 500′000 cells per well. 5 × 10⁶ Human T-Activator Dynabeads® (Gibco) per well were used for the proliferation. The different experimental conditions consisted of CM from unloaded (UL_w2) and loaded (L_w2) scaffolds from week 2 that were diluted 1:1 in the above-mentioned culture medium. Afterwards, the CM were either depleted (via immunoprecipitation) of TGF-β with a TGF-β1, 2, 3 antibody (R&D Systems) or with a Mouse IgG1 Isotype antibody (R&D Systems) as a control to determine the effect of TGF-β on T cell proliferation. The medium was refreshed with 30 U/mL IL-2 after 3 days and T cell proliferation was measured after 4 days using the FACSAria III Cell Sorter (BD). Raw data was analyzed to calculate a division index using the “Proliferation platform” within FlowJo 10.4 (FlowJo LLC) [31 ]. The division index corresponds to the number of cells at the start of culture divided by the total number of divisions.

Ladner Y.D., Menzel U., Thompson K., Armiento A.R, & Stoddart M.J. (2025). Phenotypic alterations in articulating joint cells: Role of mechanically loaded MSC secretome. Heliyon, 11(3), e42234.

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Cellular Uptake of PKH26-Labeled sEVs

2025

sEVs-TFF was labeled with PKH26 (Sigma-Aldrich) following the manufacturer’s protocol. MNT-1 cells were seeded at a density of 7,500 cells/well in a 96-well plate for 48 hours. Then, the cells were incubated with PKH26-labeled sEVs for 6 hours. After incubation, the cells were washed with PBS, fixed with 4% paraformaldehyde (Sigma-Aldrich), and stained with Hoechst dye (Sigma-Aldrich). Imaging was conducted using a fluorescence microscope (Nikon).

Kee L.T., Foo J.B., How C.W., Nur Azurah A.G., Chan H.H., Mohd Yunus M.H., Ng S.N., Ng M.H, & Law J.X. (2025). Umbilical Cord Mesenchymal Stromal Cell-Derived Small Extracellular Vesicles Modulate Skin Matrix Synthesis and Pigmentation. International Journal of Nanomedicine, 20, 1561-1578.

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Evaluating ILC2-mediated Cytotoxicity against MM

2025

To assess ILC2-mediated cytotoxicity against primary MM cells, we performed a flow cytometric and non-radioactive target-based assay. Briefly, ILC2s isolated from the PB-HD, PB and BM of MM pts were co-cultured with primary MM cells at an effector/target ratio of 1:1. DNAM-1-blocking mAb (clone F5) was also added to the PB-MM ILC2 and MM cell culture. The target cells were pre-labeled with a red fluorescent dye, PKH-26 (Sigma-Aldrich, St. Lous, MO, USA), according to the manufacturer’s instructions, to allow for their discrimination from the effector cells. After 48 h, killed target cells were identified through 7-AAD staining, which specifically permeated dead cells. Data analysis was performed by gating on the PKH-26+ target cells, followed by the analysis of the 7-AAD+ subpopulation. Target cell death was calculated based on the following ratio: [(% sample cytotoxicity − % spontaneous cytotoxicity)/(% total cytotoxicity − % spontaneous cytotoxicity)]. To evaluate ILC2 apoptosis, purified BM-ILC2s were co-cultured with autologous purified MM cells at an E:T ratio of 1:1 in the presence or absence of 5 μg/mL of anti-TIGIT neutralizing antibody (Tiragolumab Cat. No.: HY-P9986, MedChem Express, Monmouth Junction, NJ, USA) or corresponding human IgG1 kappa as an isotype control. Following 48 h of incubation, apoptotic ILC2 cells were evaluated using the Annexin V-FITC Apoptosis detection kit according to the manufacturer’s instructions (Beckman Coulter, Brea, CA, USA) within 7-AAD+/neg cells. To examine MM cell death, tumor cells were co-cultured with or without ILC2s at an E:T ratio of 1:1 in the presence or absence of 5 μg/mL of anti-TIGIT neutralizing antibody (Tiragolumab Cat. No.: HY-P9986, MedChem Express, Monmouth Junction, NJ, USA) or corresponding human IgG1 kappa as an isotype control. After 48 h, MM cell viability was evaluated as a percentage of 7-AAD+ cells within CD45negCD38+CD138+ cells.

Drommi F., Calabrò A., Pezzino G., Vento G., Freni J., Costa G., Cavaliere R., Bonaccorsi I., Allegra A., Ferlazzo G., De Pasquale C, & Campana S. (2025). Multiple Myeloma Cells Shift the Fate of Cytolytic ILC2s Towards TIGIT-Mediated Cell Death. Cancers, 17(2), 263.

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