Endo-growth media
Endo-growth media is a specialized cell culture medium designed to support the growth and maintenance of endothelial cells. It provides the essential nutrients and growth factors required for the proliferation and differentiation of endothelial cells in vitro.
4 protocols using Endo-growth media
Hypoxia-induced RPE Cell Responses
Glucose-Induced Endothelial Apoptosis
Resveratrol Modulates Apoptosis in HGECs
Anthocyanin effects on AMPK and renal cells
siRNAs targeted toward Ampkα1, Ampkα2, and Sirt1, and a non-specific scrambled siRNA (siRNA control) were complexed with a transfection reagent (Lipofectamine 2000; Invitrogen, Carlsbad, CA, USA), according to the manufacturer’s instructions. The sequences of the siRNAs were: α1-AMPK, 5′-GCAUAUGCUGCAGGUAGAU-3′; α2-AMPK, 5′-CGUCAUUGAUGAUGAGGCU-3′; SIRT1, 5′-CACCUGAGUUGGAUGAUAU-3′; and scrambled siRNA, 5′-CCUACGCCACCAAUUUCGU-3′ (Bioneer, Daejeon, Korea). At 24 h after transfection, HGECs were exposed to 40 mmol/L glucose and 50 µg/mL anthocyanins for 24 h.
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