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61 protocols using IPTT-300

Mice were pre-acclimated at thermoneutrality (28-30°C) for 1wk and then shifted to 4°C. Body temperature was measured with a rectal probe (Physitemp, RET3) and a reader (Physitemp, BAT-12) and implanted temperature probes (Bio Medic Data Systems, IPTT-300) with a transponder (Bio Medic Data Systems, DAS-7007R)34 ,35 .
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Anesthetized mice were implanted with Implantable Programmable Temperature and Identification Transponder (IPTT-300; Bio Medic Data Systems). Body temperature was measured using DAS-7009 reader (Bio Medic Data Systems).
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Body temperature was assessed in male and female mice from each of the three inbred strains (n = 10-12/strain; total = 35). All mice first were injected intraperitoneally with a non-lethal dose of E. coli LPS (5mg/kg, Sigma Chemical Company, St Louis, MO). Temperatures then were recorded with an implanted body temperature transponder (IPTT-300, Bio Medic Data Systems, Seaford DE), with readings measured using a DAS-5001 reader/programmer (Bio Medic Data Systems) from one to eight hours in one-hour time intervals.
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To monitor BAT response in real time, temperature probes (Bio Medic Data Systems, IPTT-300) were implanted into the interscapular region of mice and temperature was read with a transponder (Bio Medic Data Systems, DAS-7007R). To activate sympathetic neurons, mice were i.p. injected with saline or Clozapine N-oxide (Sigma, C0832) at 1 mg/kg.
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Mice received subscapular injection of a wireless temperature transponder (IPTT-300, Bio Medic Data Systems, Seaford, DE, USA). One week following transponder injection, mice were placed in a 4°C room for eight hours with access to food and water. Body temperatures were recorded hourly.
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In some mice, core body temperature was monitored by a miniature wireless Implantable Programmable Temperature Transponder (IPTT-300, BioMedic DataSystems) placed under the skin on the back of the animal. A previous study showed similar measurements in core temperature between this method and when an abdominal thermocouple was placed (Feketa et al., 2014 (link)).
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Body temperature was measured in mixed sex c57BL/6J (8–12 weeks old) by subcutaneous transponders (IPTT300; BioMedic Data Systems) that were implanted under isoflurane using a custom-designed injector. The temperature was checked every 20 min with BMDS Smart Probes 7000 series Reader (BioMedic Data Systems) starting from 1 h before the saline or LPS challenge up to 240 min after the treatment.
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Body composition was measured using a Bruker MiniSpec NMR. Whole-body VO2, RER, and activity levels were measured using a CLAMS system (Columbus Instruments). Cold-tolerance testing was carried out in a 4 °C cold room. Prior to cold-tolerance testing, the mice were injected with a temperature-sensitive transponder (Bio Medic Data Systems, IPTT 300). One week after the injections, the mice were transferred to a 4 °C cold room for 6–8 h, and their core temperature was assessed using a Reader-Programmer (Bio Medic Data Systems, DAS 8007). The mice were single-housed in cages containing bedding with access to food and water throughout the cold-tolerance test. For long-term cold-exposure experiments, the mice were acclimated to the cold following a protocol used to acclimate UCP1 null mice to the cold [25 (link)]. Briefly, the mice were housed in a rodent incubator at 18°C for 2 weeks, after which the temperature was lowered to 6.5°C for 7 days. The mice were euthanized after a 4 h fast and their tissues were harvested. For glucose-tolerance testing, their blood glucose levels were measured following an IP injection of 20% glucose (5 μL/g body mass) at time points 0, 15, 30, 60, and 120 min.
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At 7 weeks of age, implantable electronic transponders (IPTT300, Bio Medic Data Systems Inc., Seaford, DE) were injected subcutaneously into the mice. These were used for identification and to record body temperature by scanning the animal using the IPTT6005 scanner probe (Bio Medic Data Systems Inc.). From 8 weeks of age until death, body temperatures were recorded twice daily, corresponding to the start of light and dark periods at 08:00 (lights on) and 18:00 (lights off). Weekly averages for each time point were calculated for each animal. Eight-week body temperatures of R6/2 mice taken prior to commencement of treatment were pooled for analysis. Data are plotted as blocks of 7 days from 17 to 22 weeks of age.
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Three- to four-month old Angptl4-/-, wild-type and Angptl4-Tg mice were either placed at a thermo-neutral temperature (∼28°C) (n = 7/8, as indicated in figures) or at a cold temperature (∼4°C) (n = 7–10, as indicated in figures) for a period of 10 days. All animals are backcrossed on a pure C57Bl/6J background for multiple generations (>10). Wild-type and Angptl4-Tg mice are littermates. Angptl4-/- mice have been obtained via homologous recombination of embryonic stem cells and lack part of the Angptl4 gene, resulting in a non-functional ANGPTL4 protein (Köster et al., 2005 (link); Lichtenstein et al., 2010 (link)). Angptl4-Tg mice over-express the Angptl4 gene in various tissues under its own promoter (Mandard et al., 2006 (link)). Food intake, body weight and body temperature were monitored daily. Body temperature of cold-exposed mice was monitored via read-out of transponders (IPTT-300) that were injected subcutaneously prior to the experiment (Bio Medic Data Systems, Seaford, USA). The Animal Ethics Committees of Wageningen University and University Medical Center Hamburg-Eppendorf approved all experiments.
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