The largest database of trusted experimental protocols

Ultra sound gate cm16 cmpa microphone

Manufactured by Avisoft
Sourced in Germany

The Ultra-Sound Gate CM16/CMPA is a microphone designed for recording high-frequency acoustic signals. It features a wide frequency response range and is suitable for various applications requiring the capture of ultrasonic sounds.

Automatically generated - may contain errors

5 protocols using ultra sound gate cm16 cmpa microphone

1

Ultrasonic Vocalization Analysis in Mouse Pups

Check if the same lab product or an alternative is used in the 5 most similar protocols
On P9, mouse pup ultrasonic vocalizations (USVs) were detected for 3
min using an Ultra-Sound Gate CM16/CMPA microphone (AviSoft, Germany) and
SAS Prolab software (AviSoft, Germany) in a sound attenuation chamber under
stable temperature (19–22°C). For further characterization
of the USVs, the sonograms from the 1st minute of the recordings were
classified into ten distinct categories in accordance to previously
established methods43 and
were analyzed for the total number of calls made and the average duration of
the calls.
+ Open protocol
+ Expand
2

Ultrasonic Vocalization Analysis in Mouse Pups

Check if the same lab product or an alternative is used in the 5 most similar protocols
On P9, mouse pup ultrasonic vocalizations (USVs) were detected for 3
min using an Ultra-Sound Gate CM16/CMPA microphone (AviSoft, Germany) and
SAS Prolab software (AviSoft, Germany) in a sound attenuation chamber under
stable temperature (19–22°C). For further characterization
of the USVs, the sonograms from the 1st minute of the recordings were
classified into ten distinct categories in accordance to previously
established methods43 and
were analyzed for the total number of calls made and the average duration of
the calls.
+ Open protocol
+ Expand
3

Quantitative Analysis of Rat Ultrasonic Vocalizations

Check if the same lab product or an alternative is used in the 5 most similar protocols
Vocal emissions were recorded for the duration of the social behavior tests using an UltraSoundGate CM16/CMPA microphone (Avisoft Bioacoustics) placed just above the testing cage. The microphone was connected to a computer via an Avisoft Bioacoustics UltraSoundGate 116Hb. Acoustic data were recorded with a sampling rate of 250 kHz in 16 bit format, and spectrograms were constructed by fast Fourier transformation (FFT; 256 FFT length, 100% frame, FlatTop window, and 50% time window overlap; SASLab Pro, Avisoft Bioacoustics). All USVs made within the first 10 min of the play behavior trial were manually marked by investigators who were blind to the age, sex, and genotype of the rats. In order to be marked, calls had to be at least 10 ms in length, and distinct calls had to be separated by at least 10 ms. Several call parameters were quantified, including fundamental frequency, duration, and number of calls emitted. Call frequency (in hertz) was calculated by averaging the fundamental frequency at call onset, call offset, and peak amplitude of the call (integrated frequency). A subset (20% random sampling) of the calls was selected and manually classified into the 15 call categories described in the study by Wright et al. (2010) (link).
+ Open protocol
+ Expand
4

Behavioral Screening of Autism Traits

Check if the same lab product or an alternative is used in the 5 most similar protocols
Timed pregnant mice (E12.5) were IP injected VPA (dissolved in sterile saline) with a dosage of 600mg/kg, and saline was injected as a control.
Self-righting test was performed on VPA and saline treated cohorts using P7 pups as a measure of gross development. Briefly, animals were gently positioned supine with all four limbs pointed upwards. Latency to self-right was measured as the seconds from release until all four limbs touching ground. If the animal had not achieved this position after 60 s, the animal was returned to prone manually, and a time of 60s was recorded. The self-righting test was performed three times with one minute rest in between. The final score is the average latency of the three tests.
Ultrasonic vocalizations emitted by mouse pups can be an early indication of social awareness. On P9, the dam was removed from the housing cage and the pups were transported to a separate location for 30 minutes habituation in the testing room. Animals were then individually placed in a plastic cage, and the cage was placed within a sound-attenuating chamber. Ultrasonic vocalizations were measured for 3 minutes using an UltraSoundGateCM16/CMPA microphone (AviSoft) and SAS Prolab recording software (AviSoft). The number of vocalizations between 33–125 kHz was then counted.
+ Open protocol
+ Expand
5

Inducing Positive Affective States in Rats through Tickling

Check if the same lab product or an alternative is used in the 5 most similar protocols
USV, mostly 50-kHz-calls, are easily induced by manipulating the animal in a way that mimics the rough-and-tumble play in juvenile rats or, literally, by tickling them [111 (link)]. Tickling sessions can be used to induce playfulness and social joyfulness and the number of emitted USV may be used as a reflection and measure of positive affective states of rats [112 (link)]. Our rats were tickled for five consecutive days at PND 45–49, as described previously [113 (link),114 (link),115 (link)]. After the first three days of learning/habituation, at days 4 and 5, i.e., PND 48 and 49, the rats were taken for further analysis. Each day the rats were transported into the 58 × 37 × 20 cm tickling cage for a 30 s wait period. Then the tickling session was initiated with gentle poking of the animal’s sides, rubbing its scruff, then flipping the rat on its back and tickling it with rapid finger movements around the belly. The tickling lasted for 15 s and was followed by a 15 s period when the animal was allowed to follow experimenter’s hand. The tickling-follow scheme was repeated four times and the whole tickling session lasted for 120 s. USV were recorded with an UltraSoundGate CM16/CMPA microphone placed 30 cm above the cage, collected using Avisoft Recorder software, and analyzed using the SASLab Pro software (all from Avisoft Bioacustics, Glienicke/Nordbahn, Germany).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!