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Spectral bioimaging system

Manufactured by Applied Spectral Imaging
Sourced in United States, Israel

The Spectral bioimaging system is a laboratory equipment that captures and analyzes the spectral properties of biological samples. It is designed to non-invasively measure and map the spectral signatures of cellular and subcellular structures within a sample. The system employs advanced imaging techniques to generate high-resolution, multidimensional data about the sample's spectral characteristics.

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5 protocols using Spectral bioimaging system

For fluorescence microscopy, cells were imaged using a spectral bioimaging system (Applied Spectral Imaging, Carlsbad, CA, USA). Images were captured using a 40× air objective. All images were captured at the same exposure time to ensure uniform fluorescence intensity for comparison. Confocal images were acquired using a 63× oil objective on a Zeiss LSM 780 laser scanning microscope (Carl Zeiss, Oberkochen, Germany) or Leica SP8 confocal imaging system (Leica Microsystems, Wetzlar, Germany). The mean fluorescence intensity (MFI) of the images was measured using ImageJ (Rasband, W.S., U.S. National Institutes of Health, Bethesda, MD, USA). MFI values are represented in arbitrary units.
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After treatment with 10 ng cfChPs for 4 h, NIH3T3 cells were stained with JC-1 dye in HBSS and incubated at 37 °C for 15 min. The cells were washed twice with prewarmed HBSS. Cells were counterstained with Hoechst, mounted on glass slides, and cell-associated fluorescence was detected using a spectral bioimaging system (Applied Spectral Imaging). This experiment was performed twice.
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Cells were stained with antibodies against γ-H2AX and pATM (Supplementary Table 1), counterstained with Vectashield DAPI, and mounted on slides. The images were acquired using a spectral bioimaging system (Applied Spectral Imaging). Mean fluorescence intensity (MFI) in the cytoplasm was measured after gating out the nuclear fluorescence. This experiment was performed twice.
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Isolated mitochondria were fixed as described above and processed for immunostaining using anti-histone H4 IgG and the appropriate secondary antibodies (Supplementary Table 1). The immunostained smears were fixed with a 2% PFA-1.5% GA mixture for 5 min on ice and processed for FISH using a custom-synthesised human DNA probe (Supplementary Table 1). Briefly, immune-stained slides were hybridised overnight with a human DNA probe at 37 °C and washed once with 0.4X sodium saline citrate (SSC) at 72 °C (±2 °C) for 2 min and 4X SSC in Tween-20 twice at room temperature for 5 min each. The slides were mounted with DAPI (1 µg/ml) in HBSS, and images were acquired using a spectral bioimaging system (Applied Spectral Imaging).
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Cells were imaged using Spectral Bio-Imaging System (Applied Spectral Imaging, Israel). Images were captured using a 40x air objective on the Spectral Bio-Imaging System. The images were captured with the same exposure time, for both treated and control cells set to ensure the same conditions for comparison, while avoiding saturation and photo bleaching. For confocal microscopy, images were acquired using a 63x oil objective on Zeiss LSM 780 laser scanning microscope (Carl Zeiss, Germany) and Leica SP8 confocal imaging system (Leica Microsystems, Wetzlar, Germany).
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