Jem 1011 transmission electron microscope
The JEM 1011 is a transmission electron microscope (TEM) designed and manufactured by JEOL. It is capable of producing high-resolution images of samples at the nanoscale level. The TEM functions by transmitting a beam of electrons through a thin specimen, allowing for the observation and analysis of the internal structure and composition of materials.
Lab products found in correlation
10 protocols using jem 1011 transmission electron microscope
Ultrastructural Analysis of Peri-Infarct Cortex
Transmission Electron Microscopy Sample Preparation
Immunoelectron Microscopy of Tau Aggregates
Transmission Electron Microscopy (TEM) Tissue Fixation
Transmission Electron Microscopy of Liver Tissue
Transmission Electron Microscopy of Exosomes
Ultrastructural Analysis of CSF Samples
Cryosectioning and Dual Immunolabeling of PC12 Cells
Astrocyte Ultrastructural Preparation
Astrocyte Ultrastructural Preparation
fixed in 2.0% glutaraldehyde in 0.1 M sodium cacodylate buffer, pH 7.4
(Electron Microscopy Sciences, Hatfield, PA) for one hour at room temperature on
a rocker. They were rinsed in cacodylate buffer, gently scraped and pelleted and
post-fixed in 1.0% osmium tetroxide in cacodylate buffer for one hour on
ice. They were rinsed in buffer and stabilized with a small amount of 2%
agarose in PBS to hold them together. They were then dehydrated through a graded
series of ethanol to 100%, followed by propylene oxide, 100%.
They were infiltrated with Epon resin (Ted Pella, Redding, CA) in a 1:1 solution
of Epon:propylene oxide overnight on a rocker at room temperature. The following
day they were placed in fresh Epon for several hours and then embedded in Epon
overnight at 60 C. Thin sections were cut on a Leica EM UC7 ultramicrotome,
collected on formvar-coated grids, stained with uranyl acetate and lead citrate
and examined in a JEOL JEM 1011 transmission electron microscope at 80 kV.
Images were collected using an AMT digital imaging system (Advanced Microscopy
Techniques, Danvers, MA). These methods are similar to previous descriptions of
extracellular particle and mitochondria detection in astrocyte cultures
12 (link).
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