Next morning after PSG recordings, 5 ml of venous blood samples were collected at 8 AM under standard condition. After preparation, the serum was stored at −20°C for the following analyses according to the standard protocols.
Serum levels of DHEA-S (Code 5175-300; Monobind Inc.), sex hormone-binding globulin (SHBG) (Code 9125-300; Monobind Inc.), testosterone (Code 3725-300; Monobind Inc.), estradiol (Code 4925-300; Monobind Inc.), progesterone (Code 4825-300; Monobind Inc.), 17α-Hydroxyprogesterone (17-OH) (Code 5225-300; Monobind Inc.), follicle stimulating hormone (FSH) (Code 425-300; Monobind Inc.), and LH (Code 625-300; Monobind Inc.,) were measured by using enzyme-linked immunosorbent assay kits. The level of absorbance was read using Awareness Technology STAT FAX 2100 Microplate Reader (Awareness Technology, USA). Hormones levels were calibrated according to the standard calibration curve. Data were presented as pg/mL for estradiol, nmol/L for SHBG, mlU/mL for FSH and LH, and ng/mL for testosterone, progesterone, DHEA, and 17-OH.
Serum levels of DHEA-S (Code 5175-300; Monobind Inc.), sex hormone-binding globulin (SHBG) (Code 9125-300; Monobind Inc.), testosterone (Code 3725-300; Monobind Inc.), estradiol (Code 4925-300; Monobind Inc.), progesterone (Code 4825-300; Monobind Inc.), 17α-Hydroxyprogesterone (17-OH) (Code 5225-300; Monobind Inc.), follicle stimulating hormone (FSH) (Code 425-300; Monobind Inc.), and LH (Code 625-300; Monobind Inc.,) were measured by using enzyme-linked immunosorbent assay kits. The level of absorbance was read using Awareness Technology STAT FAX 2100 Microplate Reader (Awareness Technology, USA). Hormones levels were calibrated according to the standard calibration curve. Data were presented as pg/mL for estradiol, nmol/L for SHBG, mlU/mL for FSH and LH, and ng/mL for testosterone, progesterone, DHEA, and 17-OH.