Testosterone, progesterone, follicle stimulating hormone and luteinizing hormone assay kits were products of Monobind Inc. Lake Forest, USA. Oestraldiol valerate (Merit Healthcare Pvt Ltd. Mumbai, Indian) and metformin from (SmithKline Beecham Pharmaceuticals, West Susex UK), other chemicals and reagents used were products of Sigma Aldrich Ltd., Buchs, Canada.
Progesterone
Manufactured by Monobind
Sourced in United States
Progesterone is a laboratory equipment used for the quantitative determination of progesterone levels in human serum or plasma. It is a hormone produced primarily by the ovaries and placenta, and is essential for the regulation of the menstrual cycle and the maintenance of early pregnancy.
Automatically generated - may contain errors
Lab products found in correlation
Testosterone, by Monobind (2 mentions)
Estradiol, by Monobind (2 mentions)
Thiobarbituric acid, by Merck Group (1 mentions)
Reduced glutathione, by Merck Group (1 mentions)
Insulin, by Monobind (1 mentions)
Alcyon 300, by Abbott (1 mentions)
Stat fax 3200 microplate reader, by Awareness Technology (1 mentions)
Stat fax 2100 microplate reader, by Awareness Technology (1 mentions)
5 protocols using progesterone
1
Hormone Assays for Reproductive Health
2
Estradiol Valerate Pharmacokinetic Study
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Estradiol valerate(NAMAN PHARMA DRUGS, Mumbai.,Mfg.Lic.No.: L/19/2186/MB) was purchased from FEGTOCHI Pharmacy, Akure, Nigeria. Pentobarbital sodium (40 mg/kg, i.p.) was purchased from PASCAL (Nigeria). Thiobarbituric acid and reduced glutathione were purchased from Sigma-Aldrich Corp. (St. Louis, MO USA). Assay kits for protein are products of Randox Laboratories Limited (Co Antrim, United Kingdom) while those of testosterone, follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol, progesterone, and leptin were obtained from Monobind Inc. (California, USA). All other reagents used were of analytical grades.
3
Cumulus Cells Culture Dynamics
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Medium from cumulus cells primary culture was collected at 2, 4, 12, 24, and 48 hr after culture. AMH and Progesterone levels were measured using ELISA (AMH: AnshLabs Co., Germany; Progesterone: monobind Co., USA).
4
Hormonal and Metabolic Changes in Ewes
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Over the period of flushing, individual dry matter intake (DMI) was measured daily, and the ewes were weighed weekly before the morning meal. Blood samples were obtained from the jugular vein 24 h prior to CIDR withdrawal, 24 h after CIDR withdrawal and 9 days after CIDR withdrawal. Blood samples were collected in non-heparinised Vacutainer tubes and placed into icy water immediately following the collection. Then the serum was obtained after centrifugation at 3000 g for 15 min. The obtained serum was stored at -20 °C until subsequent analyses. Serum glucose (No. 017-500-1, Pars Azmon Co., Karaj, Iran), cholesterol (No. 010-500-1, Pars Azmon Co., Karaj, Iran) and urea (No. 1-400-030, Pars Azmon Co., Karaj, Iran) concentrations were analysed by auto-analyzer (Abbott Alcyon 300, Abbott Laboratories, Inc. Irving, TX, USA). Serum concentrations of hormones including insulin (No. 2425-300, Monobind Inc, Lake Forest, CA, USA), estradiol (No. 4925-300, Monobind Inc., Lake Forest, CA, USA) and progesterone (No. 4825-300, Monobind Inc., Lake Forest, CA, USA) were analyzed with the use of ELISA reader (Stat Fax 3200 Microplate Reader, Awareness Technology Inc., Palm City, FL, USA). All assays for analyses of blood parameters were done in accordance with the manufacturers' guidelines.
5
Serum Hormone Panel Analysis
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Next morning after PSG recordings, 5 ml of venous blood samples were collected at 8 AM under standard condition. After preparation, the serum was stored at −20°C for the following analyses according to the standard protocols.
Serum levels of DHEA-S (Code 5175-300; Monobind Inc.), sex hormone-binding globulin (SHBG) (Code 9125-300; Monobind Inc.), testosterone (Code 3725-300; Monobind Inc.), estradiol (Code 4925-300; Monobind Inc.), progesterone (Code 4825-300; Monobind Inc.), 17α-Hydroxyprogesterone (17-OH) (Code 5225-300; Monobind Inc.), follicle stimulating hormone (FSH) (Code 425-300; Monobind Inc.), and LH (Code 625-300; Monobind Inc.,) were measured by using enzyme-linked immunosorbent assay kits. The level of absorbance was read using Awareness Technology STAT FAX 2100 Microplate Reader (Awareness Technology, USA). Hormones levels were calibrated according to the standard calibration curve. Data were presented as pg/mL for estradiol, nmol/L for SHBG, mlU/mL for FSH and LH, and ng/mL for testosterone, progesterone, DHEA, and 17-OH.
Serum levels of DHEA-S (Code 5175-300; Monobind Inc.), sex hormone-binding globulin (SHBG) (Code 9125-300; Monobind Inc.), testosterone (Code 3725-300; Monobind Inc.), estradiol (Code 4925-300; Monobind Inc.), progesterone (Code 4825-300; Monobind Inc.), 17α-Hydroxyprogesterone (17-OH) (Code 5225-300; Monobind Inc.), follicle stimulating hormone (FSH) (Code 425-300; Monobind Inc.), and LH (Code 625-300; Monobind Inc.,) were measured by using enzyme-linked immunosorbent assay kits. The level of absorbance was read using Awareness Technology STAT FAX 2100 Microplate Reader (Awareness Technology, USA). Hormones levels were calibrated according to the standard calibration curve. Data were presented as pg/mL for estradiol, nmol/L for SHBG, mlU/mL for FSH and LH, and ng/mL for testosterone, progesterone, DHEA, and 17-OH.
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