GDC-0941
GDC-0941 is a potent and selective inhibitor of the PI3K (phosphoinositide 3-kinase) enzyme. It acts by blocking the activity of the PI3K pathway, which is involved in cell growth, proliferation, and survival.
Lab products found in correlation
9 protocols using GDC-0941
Evaluating CDC25 Inhibitors in AML Cells
Cell Cycle Regulation of DEK
Reagents and Antibodies for Cell Signaling
IL-2 and IL-3 were from Peprotech (Rocky Hill, NJ, USA).
The following antibodies were used for western blotting: Puma (#3043) was from Prosci (Poway, CA, USA), human Puma (#12450), Foxo3a (#2497) were from Cell Signaling Technologies (Danvers, MA, USA). GSK3a/b (sc-56913), 14-3-3 (sc-1657) and NFATc1 (sc-7294) were from Santa Cruz (Dallas, TX, USA). Tubulin (MCA77G) was from Bio-Rad (Hercules, CA, USA). MCL1 (600-401-394S) was from Rockland (Limerick, PA, USA).
FLAG-M2 agarose affinity beads were from Sigma-Aldrich (St. Louis, MO, USA).
Evaluating GDC-0941 Effects on Bladder Cancer
Cell cycle and apoptosis analysis of cells cultured with 1 μM GDC-0941 or DMSO only for 48 h was evaluated by flow cytometry as described [40 (link)]. All assays were done in triplicate and repeated at least three times.
Inhibitor Treatments and Transfection Protocols
Compound Preparation for Cell Signaling
Protein Kinase Activity Assay
Selective Kinase Inhibitors Panel
Cytotoxicity Assay for Cancer Cell Lines
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