JC-1 Mitochondrial Membrane Potential Assay Kit
The JC-1 Mitochondrial Membrane Potential Assay Kit is a laboratory product designed to measure the mitochondrial membrane potential in cells. The kit utilizes the fluorescent dye JC-1 to detect changes in the mitochondrial membrane potential.
Lab products found in correlation
11 protocols using JC-1 Mitochondrial Membrane Potential Assay Kit
JC-1 Assay for Mitochondrial Potential
Graphite-Based Cytotoxicity and Apoptosis Assays
Graphite-based Composite for Biomedical Applications
Mitochondrial Potential Quantification
Quantification of Apoptosis and Mitochondrial Membrane Potential in ZnO NP-Treated Cells
JC-1 assay was performed according to the manufacturer’s instructions (JC-1 Mitochondrial Membrane Potential Assay Kit; Abnova, Taipei City, Taiwan). Cells were cultured with 15 μg/mL ZnO NPs for 0, 3, 6, and 12 hours under the conditions described above. They were then transferred onto a coverslip housed in a four-well plate and incubated in DMEM containing 10 μM JC-1 at 37°C for 15 minutes before they were washed with PBS and rapidly mounted for observation. In all procedures, cells were mounted with Vectashield fluorescence medium and visualized under a fluorescent microscope.
Assessing Mitochondrial Membrane Potential
Mitochondrial Membrane Potential Assay
Mitochondrial Membrane Potential and Superoxide Assays
The production of superoxide by mitochondria was measured using the MitoSOX Red reagent (Thermo Fisher, Zug, Switzerland). Cells were incubated for 10 min with 5 μM MitoSOX Red and 1× SYBR (Thermo Fisher) in HBSS (Gibco), followed by three washes with HBSS. The oxidized product is highly fluorescent upon binding to nucleic acid. Bottom well fluorescence absorbance was measured using a Multimode Detector for both methods and results were normalized on RFU of SYBR.
Mitochondrial Membrane Potential in NSCs
Assessing Mitochondrial Membrane Potential
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