Triethanolamine

Name: Triethanolamine
Brand: Merck Group
SKU: mSPhCZIBPBHhf-iFqyyZ
Availability: InStock

Manufactured by Merck Group

469 citations

Sourced in United States, Germany, India, China, United Kingdom, Poland, France, Brazil, Canada, Italy, Romania, Switzerland, Indonesia, Australia

About the product

Triethanolamine is a clear, viscous liquid chemical compound. It is a primary function is to serve as a pH adjuster and emulsifier in various laboratory applications.

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Triethanolamine is a commercially available product from Merck Group and its authorized distributors. The pricing typically ranges from $25 to $100 per liter, depending on the purity and packaging. As this is an active product, there is no official replacement model.

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Lab products found in correlation

Triton x 100, by Merck Group (35 mentions) Ethanol, by Merck Group (33 mentions) Sodium hydroxide (naoh), by Merck Group (29 mentions) Methanol, by Merck Group (29 mentions) Dimethyl sulfoxide (dmso), by Merck Group (24 mentions) Acetic anhydride, by Merck Group (24 mentions) Hydrochloric acid (hcl), by Merck Group (23 mentions) Methylparaben, by Merck Group (19 mentions) Bovine serum albumin (bsa), by Merck Group (19 mentions) Urea, by Merck Group (18 mentions) Propylene glycol, by Merck Group (17 mentions) Carbopol 940, by Merck Group (16 mentions) Acetonitrile, by Merck Group (15 mentions) Sodium chloride (nacl), by Merck Group (15 mentions) Tween 80, by Merck Group (14 mentions) Oleic acid, by Merck Group (14 mentions) Tetraethyl orthosilicate, by Merck Group (14 mentions) Glycerol, by Merck Group (13 mentions) Methacrylic anhydride, by Merck Group (13 mentions) Propylparaben, by Merck Group (12 mentions)

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469 protocols using «triethanolamine»

Synthesis of Zinc Oxide Nanoparticles

2025

Tri ethanolamine (C₆H₁₅NO₃, ≥ 99%), zinc acetate
dihydrate (Zn(CH₃COO)₂.2H₂O, >99.5%),
zinc chloride (ZnCl₂, > 98%), m ethanol (CH₃OH,
≥ 99.9%), ethanol (C₂H₅OH, ≥ 99.9%),
potassium hydroxide (KOH, ≥ 99.9%), ethylene glycol (C₂H₆O₂, ≥ 99%), thiourea (CH₄N₂S, ≥ 99%) were purchased from Merck, Germany.
Tetramethylammonium hydroxide (TMAH) (C₄H₁₃NO,
≥ 97%) was obtained from Sigma-Aldrich, Germany. Carbon dioxide
gas (CO₂, ≥ 99%), and nitrogen gas (N₂, ≥ 99%) were acquired from CV Sangkuriang.

Astuti A.R., Saputera W.H., Ariono D., Wenten I.G, & Sasongko D. (2025). Enhancing CO2 Capture and Conversion to Formic Acid via a Membrane-Photocatalytic Hybrid System with ZnO–ZnS Heterojunction Catalyst. ACS Omega, 10(6), 5563-5573.

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Doxorubicin and Ferulic Acid Lipid Nanoparticles

2025

Hydrogenated soya phosphatidylcholine (HSPC) and distearylphospatidyl-ethanolamine-methoxy-(polyethylene-glycol) (DSPE-mPEG₂₀₀₀, the mean molecular weight of PEG is 2000 g/mol) were purchased from NOF Co. Ltd. (Tokyo, Japan). Cholesterol was obtained from Wako Pure Chemical Industries Inc. (Osaka, Japan). Triethanolamine was obtained from Sigma Aldrich, Co. Ltd. (Darmstadt, Germany). Doxorubicin hydrochloride (DOX) was secured from LC Laboratories (Woburn, Massachusetts), and Ferulic Acid (FA) was purchased from Tokyo Chemical Industry (Tokyo, Japan). Phosphate-buffered saline (pH 7.4) comprised 8 g sodium chloride, 0.2 g potassium chloride, 1.15 g disodium hydrogen phosphate, and 0.2 g potassium dihydrogen phosphate per liter (Oxoid Ltd., Hampshire, UK). All other chemicals used in this study were of the highest grade available.

Miatmoko A., Christy P.K., Isnaini A., Hariawan B.S., Cahyani D.M., Ahmad M., Diyah N.W., Adrianto M.F., Deevi R.K., Hamid I.S, & Ekowati J. (2025). Characterization and in vitro anticancer study of PEGylated liposome dually loaded with ferulic acid and doxorubicin. Scientific Reports, 15, 1236.

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Topical Antimicrobial Formulation Development

2025

Materials used in this experiment are as follows: Tween 80 and Span 20 (Merck NJ, United States of America), distilled water (Central Research Lab, CMUL, Idi-Araba, Lagos), chlorhexidine (Elaeis guineensis oil [Maubold Essensce, Lagos, Nigeria]), curcumin and phosphatidylcholine (Sigma-Aldrich Co., St. Louis, Missouri, United States of America), methanol (Merck, Darmstadt, Germany), phosphate buffer (Loba Chemie, Colaba, Mumbai, India), 1% cremophor (RH40) (Macklin Biochemical, Shanghai, China), Carbopol Ultrez (Surfachem, United Kingdom), distilled water, chlorhexidine (Sigma-Aldrich Co., St. Louis, Missouri, United States of America), triethanolamine (Merck, New Jersey, USA), Dettol (Reckitt Benckiser), normal saline (Unidex, Lagos, Nigeria), Mueller–Hinton agar (HiMedia Laboratories), and methylparaben and propylparaben (Sigma-Aldrich Co., St. Louis, Missouri, United States of America).

Cardoso-Daodu I.M, & Ilomuanya M.O. (2025). Development of Curcumin Encapsulated Liposomes in Chlorhexidine-Loaded Organogel Using Ternary Phase Systems for Treatment of Omphalitis in Infants. Advances in Pharmacological and Pharmaceutical Sciences, 2025, 6828052.

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Synthesis and Characterization of Ti3AlC2 Powder

2025

Ti₃AlC₂ powder (400 mesh) was purchased from Nanochemazone (Leduc, Alberta, Canada). Hydrofluoric acid (HF, 48–51%) and dimethyl sulfoxide (DMSO, ACS reagent, 99.9%) were supplied by Thermo Scientific (Fair Lawn, NJ, USA). Methanol (≥99.9%), ammonium persulfate (APS, ACS reagent, ≥98.0%), isopropyl alcohol (IPA), triethanolamine (TEA, reagent grade, 98%), thiophene (≥99%), methylene blue (MB), orange G (OG), and rhodamine B (RhB) were all obtained from Sigma-Aldrich Korea (Seoul, Republic of Korea). Cetyltrimethylammonium bromide (CTAB, 99%) was purchased from Daejung Chem (Siheung, Republic of Korea).

Bae Y.H., Hong S, & Noh J.S. (2025). Polythiophene/Ti3C2TX MXene Composites for Effective Removal of Diverse Organic Dyes via Complementary Activity of Adsorption and Photodegradation. Molecules, 30(6), 1393.

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Culturing and Infecting Leishmania infantum

2025

Leishmania infantum (MHOM/ES/92/LLM-320; isoenzyme typed MON-1) was cultured in M199 medium supplemented with 20% fetal bovine serum (Gemini Bio-Products), 100 μ/mL penicillin and 100 μg/mL streptomycin (Gibco), 2 mM L-glutamine (Gibco), 40 mM HEPEs (Gibco), 0,1 mM adenine (Sigma) in 50 mM HEPEs, 5 mg/mL hemin (Sigma) in 50% triethanolamine (Sigma), and 1 mg/mL 6-biotin (Sigma). Cultures were maintained at 26 °C. For mouse infections, metacyclic promastigotes were purified from stationary-phase cultures by Ficoll (Sigma) density gradient (8% and 20%), as adapted from Spath and Beverley^{65 (link)}, and centrifuged at 500 × g for 10 min, at 25 °C. Mice were intravenously inoculated with 3 × 10⁶ metacyclic promastigotes in 100 μl of 1 × PBS.

Pessenda G., Ferreira T.R., Paun A., Kabat J., Amaral E.P., Kamenyeva O., Gazzinelli-Guimaraes P.H., Perera S.R., Ganesan S., Lee S.H, & Sacks D.L. (2025). Kupffer cell and recruited macrophage heterogeneity orchestrate granuloma maturation and hepatic immunity in visceral leishmaniasis. Nature Communications, 16, 3125.

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Top 5 most cited protocols using «triethanolamine»

Microfluidic Hydrogel Encapsulation

Cited 6 times

Flow-focusing microfluidic geometry was utilized to form polymer droplets. Both shielding and crosslinker phases consisted of light mineral oil (Sigma) with 2% SPAN80 (Sigma). The crosslinker phase also contained an emulsion, at a ratio of 1:15, of 20 mg/mL dithiothreitol (DTT) (Sigma) in PBS. A co-flowing shielding phase protected the macromer solution – a 5% PEG-4MAL (10 kDa or 20 kDa, Laysan Bio) solution containing molecules or cells to be encapsulated – from the crosslinker phase until droplets of the macromer solution were formed. DTT rapidly diffused into macromer droplets, forming crosslinked microgels. To functionalize hydrogel with GRGDSPC (‘RGD’, AAPPTec), macromer was reacted for 20 minutes before encapsulation with 2.0 mM RGD in buffer solution containing 4 mM triethanolamine (Sigma). After formation, microgels were washed 5 times by centrifugation to remove mineral oil and excess DTT.

Headen D.M., Aubry G., Lu H, & García A.J. (2014). Microfluidic-Based Generation of Size-Controlled, Biofunctionalized Synthetic Polymer Microgels for Cell Encapsulation. Advanced materials (Deerfield Beach, Fla.), 26(19), 3003-3008.

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Corresponding organizations : Woodruff Health Sciences Center

Tau Protein Isolation and Tryptic Digestion

Cited 3 times

Aliquots (4 µg) of Tau 12 (binding region aa 9–18, Nordic Biosite), HT7 (aa 159–163, Thermo Scientific), and BT2 (aa 194–198, Thermo Scientific) were added to 50 µL/each magnetic Dynabeads M-280 and incubated 2 h on a rocking platform at room temperature. The beads were washed three times with double volume of PBS (10 mM Na-phosphate, 0.15 M NaCl, and pH 7.4). The antibody was cross-linked using 20 mM dimethyl pimelimidate dihydrochloride (DMP; Sigma-Aldrich) and 0.2 M triethanolamine (pH 8.2, Sigma-Aldrich) according to the manufacturer’s product description. The cross-linked beads were washed twice in PBS and blocked with Roti-Block (Carl Roth) for 1 h on a rocking platform at room temperature. Antibody-conjugated beads and Tween 20 (final concentration in the sample: 0.025%) were added to 3 mL CSF or 7 µL of a TBS-soluble fraction of brain homogenate diluted in 270 µL PBS. For some of the samples intended for tryptic digestion, 100 fmol of a 13C15N lysine- and arginine-labeled tau 1N4R protein standard (provided by Dr. Thomas McAvoy, Merck Research Laboratories) were added. Samples were incubated overnight on a rocking platform at + 4 °C. The magnetic beads/sample solution was transferred to a magnetic particle processor (KingFisher, Thermo Fisher Scientific) (tube 1). The following three wash steps (tubes 2–4) were conducted for 10 s in 1 mL of each washing buffer: 0.025% Tween 20 in PBS (tube 2), PBS (tube3), and 50 mM ammonium hydrogen carbonate (tube 4, pH 8.0). Tau was eluted from the beads by adding 100 µL 0.5% formic acid (tube 5) for 4 min. The eluted fractions were transferred to 0.65 mL prelubricated microcentrifuge tubes (Costar, Cat. 3206) and dried in a vacuum centrifuge. After drying, 10 µL of a solution of 1 µg trypsin in 390 µL 50 mM ammonium hydrogen carbonate was added to half of the samples and incubated overnight at + 37 °C. The reaction was stopped by adding 2 µL of 10% formic acid in ultra-pure water. The other half was analyzed intact for endogenous peptides. Samples were dried in a vacuum centrifuge and stored at − 80 °C pending analysis. Following the same protocol described above, we immunoprecipitated tau from CSF and brain lysate with the neo-epitope-specific antibodies to verify their specificity.

Cicognola C., Brinkmalm G., Wahlgren J., Portelius E., Gobom J., Cullen N.C., Hansson O., Parnetti L., Constantinescu R., Wildsmith K., Chen H.H., Beach T.G., Lashley T., Zetterberg H., Blennow K, & Höglund K. (2018). Novel tau fragments in cerebrospinal fluid: relation to tangle pathology and cognitive decline in Alzheimer’s disease. Acta Neuropathologica, 137(2), 279-296.

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Corresponding organizations : University of Gothenburg, University of Pennsylvania, Lund University, University of Perugia, Banner Sun Health Research Institute, National Hospital for Neurology and Neurosurgery, University College London, Sahlgrenska University Hospital

Photodegradable Microparticle Synthesis and Characterization

Cited 3 times

Poly (ethylene glycol) di-photodegradable-acrylate (PEGdiPDA; M_n ∼ 4,070 Da) was synthesized as previously described.^{11 (link),12 (link)} Poly (ethylene glycol) tetrathiol (PEG4SH; M_n ∼ 5,000 Da) was synthesized as previously described.^{20 (link)} Photodegradable microparticles were prepared via inverse suspension polymerization, in which PEGdiPDA was copolymerized with PEG4SH via base-catalyzed Michael addition in an aqueous phase that was suspended in an organic phase. Briefly, the organic phase was comprised of 5 ml of hexane containing 150 mg of a 3:1 ratio by weight of sorbitan monooleate (Span 80, Sigma-Aldrich) and poly (ethylene glycol)-sorbitan monooleate (Tween 80, Sigma-Aldrich).²¹ The volume of the aqueous phase was 0.25 mL comprised of 300 mM triethanolamine (Sigma-Aldrich) at pH 8.0 with 6.2 wt % of PEGdiPDA, 3.8 wt % PEG4SH, and protein. Bovine serum albumin labeled with Alexa Fluor 488 or Alexa Fluor 594 (BSA-488 or BSA-594; Invitrogen) were entrapped at 0.8 mg/ml, TGF-β1 (Peprotech) was entrapped at 0.4 μg/ml, and the fluorescently labeled Annexin-V (Invitrogen) was entrapped at 20 v/v % Annexin-V conjugate solution. All of the components of the aqueous phase except for the PEG4SH solution were combined in a 1.7 ml microcentrifuge tube while the organic phase was added to a 20 ml scintillation vial with a stir bar. To initiate polymerization, the PEG4SH was added to the aqueous phase, which was subsequently vortexed for 10 s and quickly added to the organic phase. Mixing on a stir plate formed and maintained the inverse suspension between the two phases and the polymerization was allowed to proceed overnight.
Upon completion of the polymerization, the suspension was centrifuged (Eppendorf Centrifuge Model 5702) at 1000 rcf for 10 minutes and the supernatant was decanted. The microparticles were washed twice with hexanes and recovered with the same centrifugation conditions and once in 2-propanol and centrifuged at 2000 rcf for 10 minutes. The particles were then suspended in 1× PBS and washed three times by centrifuging (Eppendorf Centrifuge Model 5418) at 16,873 rcf for 15 minutes. The recovered particles were stored in PBS at 4°C and a portion was imaged on a low vacuum scanning electron microscope (LVSEM, JSM-6480LV).

Tibbitt M.W., Han B.W., Kloxin A.M, & Anseth K.S. (2012). Synthesis and application of photodegradable microspheres for spatiotemporal control of protein delivery. Journal of biomedical materials research. Part A, 100(7), 1647-1654.

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Corresponding organizations : University of Colorado Boulder, Howard Hughes Medical Institute

Immunoprecipitation of SNAP-25 from Brain and CSF

Cited 2 times

The immunoprecipitation method for brain tissue extracts and CSF samples was performed according to Öhrfelt et al. with minor modifications
[39 (link)]. Briefly, an aliquot (1 μg) of the mouse monoclonal antibody SP12 (1 g/L) or the mouse monoclonal antibody SMI81 (1 g/L) or IgG from murine serum (1 g/L, Sigma-Aldrich) (a negative control), was separately added to 100 μL magnetic Dynabeads M-280 Sheep anti-mouse IgG (Invitrogen Corporation) and incubated 1 h on a rocking platform at room temperature. The beads were washed three times with 1 mL of PBS (10 mM Na-phosphate, 0.15 M NaCl, pH 7.4). The antibodies were cross-linked using 20 mM dimethyl pimelimidate dihydrochloride (Sigma-Aldrich) and 0.2 M triethanolamine (pH 8.2 Sigma-Aldrich) according to the manufacturer’s product description. The cross-linked beads were washed two times in PBS and were blocked with Roti-Block (Carl Roth) for 1 h on a rocking platform at room temperature. Each brain tissue extract (soluble, membrane-bound, and membrane-raft associated proteins) (26 μg of total protein) and CSF samples (German cohort, 890 μL; Swedish cohort I, 700 μL; Swedish cohort II, 600 μL) were adjusted with 20% Triton and PBS to a final concentration of 0.2% Triton and a final volume of 1 mL). Samples and magnetic beads were incubated overnight on a rocking platform at +4°C. The magnetic beads/sample solution was transferred to the KingFisher magnetic particle processor (Thermo Fisher Scientific), tube 1. The following three wash steps (tubes 2-4) were conducted for 10 s in 1 mL of each washing buffer: (tube 2) 0.025% Tween 20 in PBS, (tube 3) PBS and (tube 4) 50 mM ammonium hydrogen carbonate (NH₄HCO₃, pH 8.0). SNAP-25 was then eluted from the beads by adding 100 μL 0.5% formic acid (FA) (tube 5) for 4 min. The eluted fractions were transferred to 0.5 mL Protein LoBind Tube (Eppendorf AG) and dried in a vacuum centrifuge.

Brinkmalm A., Brinkmalm G., Honer W.G., Frölich L., Hausner L., Minthon L., Hansson O., Wallin A., Zetterberg H., Blennow K, & Öhrfelt A. (2014). SNAP-25 is a promising novel cerebrospinal fluid biomarker for synapse degeneration in Alzheimer’s disease. Molecular Neurodegeneration, 9, 53.

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Corresponding organizations : University of Gothenburg, University of British Columbia, Central Institute of Mental Health, Lund University, University College London, National Hospital for Neurology and Neurosurgery

Immunoblotting of Tissue Samples

Cited 2 times

Tissue samples for immunoblotting were placed in 10 ml ice-cold isolation solution, containing 250 mM sucrose, 10 mM triethanolamine (Sigma-Aldrich, St. Louis, MO, USA), 1 mg/ml leupeptin (Sigma-Aldrich) and 0.1 mg/ml phenylmethylsulfonyl fluoride (Sigma-Aldrich) titrated to pH 7.6, and the mixture was homogenized at 13,600 × g with three strokes for 15 sec using a tissue homogenizer (PowerGun 125; Thermo Fisher Scientific, Pittsburgh, PA, USA). Following homogenization, the total protein concentration was measured using a bicinchoninic acid protein assay reagent kit (Thermo Fisher Scientific, Rockford, IL, USA), which was adjusted to 2 mg/ml with isolation solution. Equal amounts of protein and sample buffer were separated using 12% gradient SDS-PAGE, stained with Coomassie Brilliant Blue and transferred to a polyvinylidene fluoride membrane. The blotted membrane was blocked with Tris-buffered saline containing 5% milk, and incubated with HIF-1α, VEGF or CD34 rabbit polyclonal antibodies (1:500; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), followed by incubation with a HRP-coupled secondary antibody (1:1000; Cell Signaling Technology, Inc., Danvers, MA, USA). The proteins were detected using enhanced chemiluminescence (Thermo Fisher Scientific). All immunoblots were representative of at least three independent experiments.

FU S., BAI R., ZHAO Z., ZHANG Z., ZHANG G., WANG Y., WANG Y., JIANG D, & ZHU D. (2014). Overexpression of hypoxia-inducible factor-1α and vascular endothelial growth factor in sacral giant cell tumors and the correlation with tumor microvessel density. Experimental and Therapeutic Medicine, 8(5), 1453-1458.

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Corresponding organizations : Chongqing Medical University, Inner Mongolia Medical University, First Affiliated Hospital of Chongqing Medical University, Fourth Hospital of Inner Mongolia

Spelling variants (same manufacturer)

Triethanolamine (TEA) - 41 citations Triethanolamine hydrochloride - 22 citations Triethanolamine buffer - 5 citations Triethanolamine buffer solution - 4 citations Triethanolamine (C6H15NO3, - 2 citations Triethanolamine (TEOHA) - 2 citations Triethanolamine (TEA) 98% solution - 2 citations Triethanolamine (TEAH3) - 2 citations

Similar products (other manufacturers)

Triethanolamine (TEA; (by Thermo Fisher Scientific) - 6 citations Triethanolamine (TEOA) (by Sinopharm) - 4 citations Triethanolamine(TEOA (by Thermo Fisher Scientific) - 4 citations Triethanolamine (TEA) (by Sinopharm) - 4 citations

The spelling variants listed above correspond to different ways the product may be referred to in scientific literature.
These variants have been automatically detected by our extraction engine, which groups similar formulations based on semantic similarity.

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