The largest database of trusted experimental protocols

Amt image capture engine v602

Sourced in United States

The AMT Image Capture Engine V602 software is a digital imaging solution designed to facilitate the acquisition and processing of high-quality microscopic images. The software provides a user-friendly interface for controlling image capture parameters and enables seamless integration with a variety of microscopy hardware.

Automatically generated - may contain errors

48 protocols using amt image capture engine v602

1

Ultrastructural Analysis of Biological Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
For ultrastructural analyses, samples were fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences, Warrington, PA) in 100 mM sodium cacodylate buffer, pH 7.2 for 2 hours at room temperature and then overnight at 4°C. Samples were washed in sodium cacodylate buffer at room temperature and postfixed in 1% osmium tetroxide (Polysciences) for 1 hour. Samples were then rinsed extensively in dH20 prior to en bloc staining with 1% aqueous uranyl acetate (Ted Pella, Redding, CA) for 1 hour. Following several rinses in dH20, samples were dehydrated in a graded series of ethanol and embedded in Eponate 12 resin (Ted Pella). Sections of 95 nm were cut with a Leica Ultracut UCT ultramicrotome (Leica Microsystems, Bannockburn, IL), stained with uranyl acetate and lead citrate, and viewed on a JEOL 1200 EX transmission electron microscope (JEOL USA, Peabody, MA) equipped with an AMT 8-MP digital camera and AMT Image Capture Engine V602 software (Advanced Microscopy Techniques, Woburn, MA).
+ Open protocol
+ Expand
2

Biofilm Ultrastructural Analysis by TEM

Check if the same lab product or an alternative is used in the 5 most similar protocols
Biofilm grown on urinary catheter surface was processed for structure characterization using transmission electron microscopy (TEM) (84 (link)). For microstructural characterization, 1 cm catheter with biofilm formed on the inside surface was fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc.) in 100 mM sodium cacodylate buffer (pH 7.2) for 1 hour at room temperature. Samples were washed in sodium cacodylate buffer and postfixed in 1% osmium tetroxide (Polysciences Inc.) for 1 hour. Samples were then rinsed extensively in deionized water prior to en bloc staining with 1% aqueous uranyl acetate (Ted Pella Inc.) for 1 hour. Following several rinses in deionized water, samples were dehydrated in a graded series of ethanol and embedded in Eponate 12 resin (Ted Pella Inc.). Sections of 95 nm were cut with a Leica Ultracut UCT ultramicrotome (Leica Microsystems Inc.), stained with uranyl acetate and lead citrate, and viewed on a JEOL 1200 EX transmission electron microscope (JEOL USA Inc.) equipped with an AMT 8 megapixel digital camera and AMT Image Capture Engine V602 software (Advanced Microscopy Techniques).
+ Open protocol
+ Expand
3

Transmission Electron Microscopy of Mycobacterium smegmatis

Check if the same lab product or an alternative is used in the 5 most similar protocols
M. smegmatis was fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc.) in 100 mM sodium cocadylate buffer, pH 7.2 for 1 hour at room temperature. Samples were washed in sodium cacodylate buffer and postfixed in 1% osmium tetroxide (Polysciences Inc.) for 1 hr. Samples were then rinsed extensively in dH20 prior to en bloc staining with 1% aqueous uranyl acetate (Ted Pella Inc.) for 1 hr. Following several rinses in dH20, samples were dehydrated in a graded series of ethanol and embedded in Eponate 12 resin (Ted Pella Inc.). Sections of 95 nm were cut with a Leica Ultracut UCT ultramicrotome (Leica Microsystems Inc.), stained with uranyl acetate and lead citrate, and viewed on a JEOL 1200 EX transmission electron microscope (JEOL USA Inc.) equipped with an AMT 8 megapixel digital camera and AMT Image Capture Engine V602 software (Advanced Microscopy Techniques).
+ Open protocol
+ Expand
4

Ultrastructural Analysis of Infected Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
For ultrastructural analyses, infected cells were fixed in a freshly prepared mixture of 1% glutaraldehyde (Polysciences Inc.) and 1 % osmium tetroxide (Polysciences Inc.) in 50 mM phosphate buffer at 4 °C for 45 min. The low osmolarity fixative was used to dilute soluble cytosolic proteins and enhance the visualization of cytoskeletal and conoid structure. Samples were then rinsed extensively in cold dH2O prior to en bloc staining with 1 % aqueous uranyl acetate (Ted Pella Inc.) at 4 °C for 3 h. Following several rinses in dH2O, samples were dehydrated in a graded series of ethanol and embedded in Eponate 12 resin (Ted Pella Inc.). Sections of 95 nm were cut with a Leica Ultracut UCT ultramicrotome (Leica Microsystems Inc., Bannockburn, IL), stained with uranyl acetate and lead citrate, and viewed on a JEOL 1200 EX transmission electron microscope (JEOL USA Inc.) equipped with an AMT 8 megapixel digital camera and AMT Image Capture Engine V602 software (Advanced Microscopy Techniques).
+ Open protocol
+ Expand
5

Ultrastructural Analysis of A. urinae

Check if the same lab product or an alternative is used in the 5 most similar protocols
For ultrastructural analyses, A. urinae cells prepared in PBS (as for mouse inoculations) were spun at 500 rpm; pellets were fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA, USA) in 100 mM sodium cacodylate buffer, pH 7.2 for 1 h at room temperature. Samples were washed in sodium cacodylate buffer at room temperature and postfixed in 1% osmium tetroxide (Polysciences Inc.) for 1 h. Samples were then rinsed extensively in dH2O prior to en bloc staining with 1% aqueous uranyl acetate (Ted Pella Inc., Redding, CA, USA) for 1 h. Following several rinses in dH2O, samples were dehydrated in a graded series of ethanol and embedded in Eponate 12 resin (Ted Pella Inc.). Sections of 95 nm were cut with a Leica Ultracut UCT ultramicrotome (Leica Microsystems Inc., Bannockburn, IL, USA), stained with uranyl acetate and lead citrate, and viewed on a JEOL 1200 EX transmission electron microscope (JEOL USA Inc., Peabody, MA, USA) equipped with an AMT 8-megapixel digital camera and AMT Image Capture Engine V602 software (Advanced Microscopy Techniques, Woburn, MA, USA).
+ Open protocol
+ Expand
6

Detailed Imaging Protocols for Parasite Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Parasites monitored by
thin smear were dyed using Harleco Hemacolor stains (MilliporeSigma).
Images were taken using a Zeiss Axio Observer.D1 at the Washington
University Molecular Microbiology Imaging Facility. For transmission
electron microscopy, infected RBCs were fixed in 2% paraformaldehyde/2.5%
glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM sodium
cacodylate buffer, pH 7.2 for 1 h at room temperature. Samples were
washed in sodium cacodylate buffer at room temperature and postfixed
in 1% osmium tetroxide (Polysciences Inc.) for 1 h. Samples were rinsed
extensively in deionized water prior to en bloc staining
with 1% aqueous uranyl acetate (Ted Pella Inc., Redding, CA) for 1
h. Following several rinses in deionized water, samples were dehydrated
in a graded series of ethanol and embedded in Eponate 12 resin (Ted
Pella Inc.). Sections of 95 nm were cut with a Leica Ultracut UCT
ultramicrotome (Leica Microsystems Inc., Bannockburn, IL), stained
with uranyl acetate and lead citrate, and viewed on a JEOL 1200 EX
transmission electron microscope (JEOL USA Inc., Peabody, MA) equipped
with an AMT 8-megapixel digital camera and AMT Image Capture Engine
V602 software (Advanced Microscopy Techniques, Woburn, MA).
+ Open protocol
+ Expand
7

Ultrastructural Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
For ultrastructural analyses, cells were fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences, Inc., Warrington, PA, USA) in 100 mM sodium cacodylate buffer, pH 7.2, for 2 h at room temperature and then overnight at 4°C. Samples were washed in sodium cacodylate buffer and post-fixed in 1% osmium tetroxide (Polysciences, Inc.) for 1 h at room temperature. Samples were then rinsed extensively in dH2O prior to en bloc staining with 1% aqueous uranyl acetate (Ted Pella, Inc., Redding, CA, USA) for 1 h. Following several rinses in dH2O, samples were dehydrated in a graded series of ethanol and embedded in Eponate 12 resin (Ted Pella, Inc.). Sections of 95 nm were cut with a Leica Ultracut UC7 ultramicrotome (Leica Microsystems, Inc., Bannockburn, IL, USA), stained with uranyl acetate and lead citrate, and viewed on a JEOL 1200 EX transmission electron microscope (JEOL USA, Inc., Peabody, MA, USA) equipped with an AMT 8-megapixel digital camera and AMT Image Capture Engine V602 software (Advanced Microscopy Techniques, Woburn, MA, USA).
+ Open protocol
+ Expand
8

Ultrastructural Analysis of ALI-Cultured Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
For ultrastructural analyses, ALI cultures were fixed in a freshly prepared mixture of 1% glutaraldehyde (Polysciences, Inc) and 1% osmium tetroxide (Polysciences, Inc.) in 50 mM phosphate buffer at 4°C for 30 min. Samples were then rinsed multiple times in cold dH20 prior to en bloc staining with 1% aqueous uranyl acetate (Ted Pella Inc.) at 4°C for 3 hr. Transwell membranes were removed from insert using a scalpel. Following several rinses in dH20, samples were dehydrated in a graded series of ethanol and embedded in Eponate 12 resin (Ted Pella, Inc.). Sections of 95 nm were cut with a Leica Ultracut UCT ultramicrotome (Leica Microsystems, Inc.), stained with uranyl acetate and lead citrate, and viewed on a JEOL 1200 EX transmission electron microscope (JEOL USA, Inc.) equipped with an AMT 8-megapixel digital camera and AMT Image Capture Engine V602 software (Advanced Microscopy Techniques).
+ Open protocol
+ Expand
9

Ultrastructural Analysis of Infected Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
For ultrastructural analyses, infected cells were fixed in 2% paraformaldehyde (Electron Microscope Sciences, catalog no. 15710)–2.5% glutaraldehyde (Poly Scientific R&D Corp., catalog no. S1809-8oz)–100 mM sodium cacodylate buffer (Electron Microscopy Sciences, catalog no. 11654) (pH 7.2) for 1 h at room temperature. Samples were washed in sodium cacodylate buffer, embedded in a thin layer of 2.5% agarose, and postfixed in 1% osmium tetroxide (Polysciences Inc.) for 1 h. Samples were then rinsed extensively in double-distilled water (dH2O) prior to en bloc staining with 1% aqueous uranyl acetate (Ted Pella Inc., Redding, CA) for 1 h. Following several rinses in dH2O, samples were dehydrated in a graded ethanol series and embedded in Eponate 12 resin (Ted Pella Inc.). Sections of 95 nm were cut with a Leica Ultracut UCT ultramicrotome (Leica Microsystems Inc., Bannockburn, IL), stained with uranyl acetate and lead citrate, and viewed on a Jeol 1200 EX transmission electron microscope (Jeol USA Inc., Peabody, MA) equipped with an Advanced Microscopy Techniques (AMT) 8-megapixel digital camera and AMT Image Capture Engine V602 software (Advanced Microscopy Techniques, Woburn, MA).
+ Open protocol
+ Expand
10

Ultrastructural Analysis of Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
For ultrastructural analyses, cells were fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences, Inc., Warrington, PA, USA) in 100 mM sodium cacodylate buffer, pH 7.2, for 2 h at room temperature and then overnight at 4°C. Samples were washed in sodium cacodylate buffer and post-fixed in 1% osmium tetroxide (Polysciences, Inc.) for 1 h at room temperature. Samples were then rinsed extensively in dH2O prior to en bloc staining with 1% aqueous uranyl acetate (Ted Pella, Inc., Redding, CA, USA) for 1 h. Following several rinses in dH2O, samples were dehydrated in a graded series of ethanol and embedded in Eponate 12 resin (Ted Pella, Inc.). Sections of 95 nm were cut with a Leica Ultracut UC7 ultramicrotome (Leica Microsystems, Inc., Bannockburn, IL, USA), stained with uranyl acetate and lead citrate, and viewed on a JEOL 1200 EX transmission electron microscope (JEOL USA, Inc., Peabody, MA, USA) equipped with an AMT 8-megapixel digital camera and AMT Image Capture Engine V602 software (Advanced Microscopy Techniques, Woburn, MA, USA).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!