All cell lines were cultured in media supplemented with 10% Heat inactivated FBS (ThermoFisher, 16140071) and 1% Pen/Strep (ThermoFisher, 15070063) at 37°C/90% RH/5% CO2, except for MDA-MB-468, which was cultured at 0% CO2. All cell lines are immortalized human cell lines, except for CT26, which is an immortalized mouse cell line. Sex, media, and RRID for each cell line: HAP1 [sex: male, media: IMDM (ThermoFisher, 31980030), RRID: CVCL_Y019]; HCT116 [sex: male, media: McCoy’s 5A (ThermoFisher, 16600108), RRID: CVCL_0291]; HT-29 [sex: female, media: McCoy’s 5A, RRID: CVCL_0320]; HCC1143 [sex: female, media: RPMI 1640 (ThermoFisher, A1049101), RRID: CVCL_1245]; A549 [sex: male, media: DMEM (ThermoFisher, 10569010), RRID: CVCL_0023]; HCC366 [sex: female, media: RPMI 1640, RRID: CVCL_2059]; HCC1954 [sex: female, media: RPMI 1640, RRID: CVCL_1259]; SKBR3 [sex: female, media: McCoy’s 5A, RRID: CVCL_0033]; MDA-MB-468 [sex: female, media: Leibovitz L-15 (ThermoFisher, 11415064), RRID: CVCL_0419]; NCI-H1650 [sex: male, media: RPMI 1640, RRID: CVCL_1483]; CT26 [sex: female, media: RPMI 1640, RRID: CVCL_7254]. Cell lines were tested for mycoplasma upon arrival at Pfizer and authenticated by STR analysis internally at Pfizer and/or by ATCC.
Rpmi 1640
Manufactured by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, France, Canada, Japan, Australia, Italy, Switzerland, Belgium, New Zealand, Austria, Netherlands, Israel, Sweden, Denmark, India, Ireland, Spain, Brazil, Norway, Argentina, Macao, Poland, Holy See (Vatican City State), Mexico, Hong Kong, Portugal, Cameroon
RPMI 1640 is a common cell culture medium used for the in vitro cultivation of a variety of cells, including human and animal cells. It provides a balanced salt solution and a source of essential nutrients and growth factors to support cell growth and proliferation.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (5958 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2641 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2403 mentions)
Penicillin, by Thermo Fisher Scientific (1792 mentions)
Streptomycin, by Thermo Fisher Scientific (1785 mentions)
L glutamine, by Thermo Fisher Scientific (1037 mentions)
Fetal calf serum (fcs), by Thermo Fisher Scientific (449 mentions)
Fetal bovine serum (fbs), by Merck Group (399 mentions)
Glutamax, by Thermo Fisher Scientific (399 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (383 mentions)
Hepes, by Thermo Fisher Scientific (377 mentions)
Sodium pyruvate, by Thermo Fisher Scientific (366 mentions)
Dmem f12, by Thermo Fisher Scientific (321 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (296 mentions)
Non essential amino acids (neaa), by Thermo Fisher Scientific (251 mentions)
Streptomycin, by Merck Group (243 mentions)
Penicillin streptomycin, by Merck Group (233 mentions)
Pen strep, by Thermo Fisher Scientific (228 mentions)
Penicillin, by Merck Group (227 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (205 mentions)
15 891 protocols using rpmi 1640
1
Cell Line Culture Conditions
2
Detailed T Cell Culture Media Protocols
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Media (R10) for washing PBMC consisted of RPMI 1640 (Gibco, Waltham, MA) supplemented with 10% fetal calf serum (Hyclone, Logan, UT). Our base T cell media (TCM) consisted of sterile-filtered RPMI 1640 supplemented with 10% fetal calf serum, 100 U/ml Penicillin, 100 mg/ml Streptomycin, 55 mM 2-mercaptoethanol, 0.3X Essential Amino Acids, 60 mM Non-essential Amino Acids, 11 mM HEPES, and 800 mM L-Glutamine (Gibco, Waltham, MA). Our TCM containing human serum (TCM/HS) consisted of sterile-filtered RPMI 1640 supplemented with 10% human serum (derived from healthy donors), 100 U/ml Penicillin, 100 mg/ml Streptomycin, and 400 mM L-Glutamine (Gibco, Waltham, MA). For culture of Jurkat cells, enhanced RPMI was used, which consisted of RPMI 1640 (Gibco, Waltham, MA) supplemented with 10% fetal calf serum (Hyclone, Logan, UT), 100 U/ml Penicillin, 100 mg/ml Streptomycin, and 800 mM L-Glutamine (Gibco, Waltham, MA).
3
Single-Cell Isolation from Solid Tumors and Spleens
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tumor tissue was digested in the RPMI 1640 medium (Gibco) containing DNAse (60 µg/ml), hyaluronidase (0.2 µl/ml), Collagenase IA and IV (0.2 mg/ml each) for 30 min at 37 °C. Required enzymes were obtained from Sigma. Cell solutions were subsequently washed with RPMI and filtered using a 40 -µm cell strainer to obtain single-cell preparations.
Splenocytes were released from spleens by passing through a 40 -µm cell strainer and washed with RPMI 1640 (Gibco). Erythrocytes were lysed by adding 1x EBC lysis buffer (BioLegend) for 5 min at 4 °C, and cells were subsequently washed with RPMI 1640. Cells were resuspended in the RPMI 1640 medium supplemented with 2% FCS, 100 U/mL streptomycin, 100 mg/mL penicillin, 1% MEM with nonessential amino acids (×100 solution, Gibco) ß-mercaptoethanol (50 µM, Sigma), and sodium pyruvate (1 mM, Gibco), and kept at 37 °C and 5% CO2 overnight during peptide stimulation.
Erythrocytes were removed from blood samples by adding ×1 EBC lysis buffer (BioLegend) and incubated for 3 min at 4 °C. Cells were subsequently washed with RPMI 1640 (Gibco).
Splenocytes were released from spleens by passing through a 40 -µm cell strainer and washed with RPMI 1640 (Gibco). Erythrocytes were lysed by adding 1x EBC lysis buffer (BioLegend) for 5 min at 4 °C, and cells were subsequently washed with RPMI 1640. Cells were resuspended in the RPMI 1640 medium supplemented with 2% FCS, 100 U/mL streptomycin, 100 mg/mL penicillin, 1% MEM with nonessential amino acids (×100 solution, Gibco) ß-mercaptoethanol (50 µM, Sigma), and sodium pyruvate (1 mM, Gibco), and kept at 37 °C and 5% CO2 overnight during peptide stimulation.
Erythrocytes were removed from blood samples by adding ×1 EBC lysis buffer (BioLegend) and incubated for 3 min at 4 °C. Cells were subsequently washed with RPMI 1640 (Gibco).
4
PBMC and T cell culture media
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Media (R10) for washing PBMC consisted of RPMI 1640 (Gibco, Waltham, MA) supplemented with 10% fetal calf serum (Hyclone, Logan, UT). Our base T cell media (TCM) consisted of sterile-filtered RPMI 1640 supplemented with 10% fetal calf serum, 100 U/ml Penicillin, 100 mg/ml Streptomycin, 55 mM 2-mercaptoethanol, 0.3X Essential Amino Acids, 60 mM Non-essential Amino Acids, 11 mM HEPES, and 800 mM L-Glutamine (Gibco, Waltham, MA). Our TCM containing human serum (TCM/HS) consisted of sterile-filtered RPMI 1640 supplemented with 10% human serum (derived from healthy donors), 100 U/ml Penicillin, 100 mg/ml Streptomycin, and 400 mM L-Glutamine (Gibco, Waltham, MA). For culture of Jurkat cells, enhanced RPMI was used, which consisted of RPMI 1640 (Gibco, Waltham, MA) supplemented with 10% fetal calf serum (Hyclone, Logan, UT), 100 U/ml Penicillin, 100 mg/ml Streptomycin, and 800 mM L-Glutamine (Gibco, Waltham, MA).
5
Brusatol-Induced Apoptosis Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Brusatol (BT) was provided by Professor Zhi-Xiu Lin. The stock solution of BT (10 mM) was prepared in dimethyl sulfoxide, stored at −80 ℃, and diluted in cell culture medium for use. Dimethyl sulfoxide (DMSO), 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Sodium dodecyl sulfate (SDS), and ribonuclease A from bovine pancreas were purchased from Sigma–Aldrich (St. Louis, MO, USA). Bovine serum albumin was purchased from Biosesang (Sungnam, Korea). RPMI1640, DMEM/low, MEM media, fetal bovine serum (FBS), and antibiotic-antimycotic mixture were obtained from Thermo Scientific HyClone (Waltham, MA, USA). FITC Annexin V Apoptosis Detection Kit I was purchased from BD Biosciences (San Diego, CA, USA). Caspase-3 inhibitor Z-DEVD-FMK was purchased from Calbiochem (San Diego, CA, USA).
6
Evaluation of JNK/p38 MAPK Signaling in EV71 Infection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS) and RPMI 1640 were purchased from Thermo Scientific HyClone (UT, USA). Hybond C membrane and ECL Western blot detection system were from Pierce (Rockford, IL, USA). Rabbit polyclonal antibodies against JNK, p-JNK, p38 MAPK, p-p38 MAPK, c-Fos, p-c-Fos, c-Jun, p-c-Jun and horseradish peroxidase (HRP) conjugated goat anti-rabbit IgG were purchased from SAB (Pearland, TX, USA). Antibodies against anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were obtained from ProteinTECH Group (Chicago, IL, USA). Rabbit polyclonal antibody against EV71 VP1 was purchased from Abcam (Cambridge, UK). The JNK1/2 and p38 MAPK specific inhibitor (SP600125 and SB203580) were acquired from LC Laboratories (Woburn, MA, USA) and freshly prepared using DMSO solution.
7
Cell Culture Media Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Dulbecco modified Eagle medium (DMEM) supplemented with 4.5 g glucose/L and 300 mg/L L −glutamine was purchased from Hyclone Laboratories Inc, Logan, Utah. Roswell Park Memorial Institute 1640 (RPMI-1640) was purchased from Thermo Scientific Hyclone, USA. Fetal bovine serum (FBS) was purchased from Lonza Inc. (Allendale, New Jersey, USA). Keratinocyte serum-free medium (KSFM) and TRIzol reagent were obtained from Invitrogen, Grand Island, New York. Minimum essential medium alpha (MEM-α) was purchased from Life Technologies, USA. Cisplatin and 3–(4, 5-dimethylthiazol-2-gl)-2, 5-diphenyl-tetrazoliumbromide (MTT) reagents were obtained from EMD Chemicals Inc. Middlebrook 7H10 agar and 7H9 broth were obtained from Sigma-Aldrich, Germany. Mycobacterium indicus pranii was provided by Prof. Dr. Niyaz Ahmed, Department of Biotechnology & Bioinformatics, School of Life Sciences, University Hyderabad, India.
8
Culturing CAOV3 and HUVEC Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human EOC cell line-CAOV3 cells were cultured in RPMI1640 containing 10% fetal bovine serum (Thermo Scientific Hyclone, USA) and penicillin/streptomycin (100 U/ml). Cells were maintained at 37 °C in a humidified atmosphere of 5% CO2. When 80% confluent, cells were treated with ADM (100 nM, Phoenix Pharmaceuticals, USA) or ADM22-52 (1 nM, Phoenix Pharmaceuticals, USA) for 24 h.
Human umbilical vein endothelial cells (HUVECs) were freshly isolated as described previously29 (link), from human umbilical veins of newborn obtained from a parturient at Shenyang 242 Hospital who gave written informed consent. HUVECs were routinely grown in DMEM (Gibco, USA) supplemented with 10% fetal bovine serum (Gibco, USA) and endothelial cell growth supplement (BD Biosciences, USA) at 37 °C and 5% CO2. In our experiments, only the first three passages of each HUVECs primary cultured were used.
Human umbilical vein endothelial cells (HUVECs) were freshly isolated as described previously29 (link), from human umbilical veins of newborn obtained from a parturient at Shenyang 242 Hospital who gave written informed consent. HUVECs were routinely grown in DMEM (Gibco, USA) supplemented with 10% fetal bovine serum (Gibco, USA) and endothelial cell growth supplement (BD Biosciences, USA) at 37 °C and 5% CO2. In our experiments, only the first three passages of each HUVECs primary cultured were used.
9
Cell Culture Conditions for Pancreatic Cancer Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All cell lines were purchased from American Type Culture Collection (ATCC, Manassas, VA). The AsPC-1, BxPC-3, MIA Paca 2, and Panc-1 cells were grown in Dulbeccos Modified Eagles Medium (DMEM, Thermo Scientific Hyclone, Waltham, MA) with 10% fetal bovine serum (FBS, ATLAS Biologicals, Fort Collins, CO), Capan-1 in DMEM with 20% FBS, and Panc 03.27 in RPMI 1640 (Thermo Scientific Hyclone, Waltham, MA) with 15% FBS and human recombinant insulin (10 units/ml, Sigma Aldrich, St. Louis, MO) in the presence of 5% CO2 at 37°C.
10
Cell Culture Conditions for Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
BGC-823 and SGC-7901 cells were respectively cultured in Dulbecco’s Modified Eagle Medium and RPMI-1640 (Thermo Scientific HyClone, Beijing, China) supplemented with 10% fetal bovine serum (FBS), 100 U penicillin, and 100 μg/ml streptomycin (Gibco of Thermo Fisher Scientific Inc., Waltham, MA, USA) at 37 °C in an atmosphere of 5% CO2.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!