EASYspin Plus Complex Plant RNA Kit
The EASYspin Plus Complex Plant RNA Kit is a laboratory equipment product designed for the isolation and purification of high-quality RNA from complex plant samples. It utilizes a rapid and efficient spin column-based method to extract RNA, including from challenging plant materials.
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36 protocols using EASYspin Plus Complex Plant RNA Kit
Robust RNA Extraction and Characterization
Genomic DNA and Total RNA Extraction
Total RNA was extracted from fresh tissues collected at different growth stages using the EASYspin Plus Complex Plant RNA Kit (Aidlab, Beijing, China). First-strand cDNA was synthesized with the AMV First-Strand cDNA Synthesis Kit (Sangon Biotech, Shanghai, China).
Isolation and Cloning of PeWRKY1 Gene
qRT-PCR Analysis of Longan Actin Gene
Identification of Viral RNA Transcripts
Total RNA Extraction and Sequencing
RNA-seq and de novo assembly protocol
RNA-Seq Analysis of Cotton Petal Development
Quantitative RT-PCR Analysis of Plant Defense
Defense suppression tests were performed as previously reported (Chen et al., 2015 (link)). Briefly, 10-day-old seedlings of N. benthamiana were treated with 1 µM flg22 (Sangon Biotech, China). The expression of the PTI-associated genes NbACRE31, NbGRAS2, and NbPTI5 were determined by qRT-PCR. In addition, qRT-PCR was also used to determine the expression levels of the defense-related genes in the SA and jasmonic acid (JA) signaling pathways, including PATHOGENESIS RELATED PROTEIN 1 (NbPR1) and LINOLEATE 9S-LIPOXYGENASE 5 (NbLOX), which are specifically induced by SA signaling. The NbEF1α and NbTUBULIN6 genes were used as internal controls.
Total RNA Extraction and RNA-seq Analysis
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