Calcein-AM
Calcein-AM is a fluorescent dye used for cell viability and cytotoxicity assays. It is a non-fluorescent, cell-permeable compound that is converted to a green-fluorescent calcein upon hydrolysis by intracellular esterases in living cells.
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28 protocols using Calcein-AM
Calcein-based Cell Viability Assay
Caspase-3/7 and Viability Assay
Calcein-AM Acini Fluorescence Imaging
Assessing Cytotoxicity and Apoptosis
Measuring MSC Adhesion Dynamics
Neutrophil Migration and Adhesion Assay
Evaluating Cell Viability of Coleman Fat and ACF
The explant culture was performed according to a standard protocol (Sineh et al., 2020 (link)). Briefly, the Coleman fat and ACF were evenly distributed on the surface of 100 mm culture dishes (1 ml/dish). The samples were cultured in complete growth medium (HUXMD-90011, Cyagen, China) at 37°C with 5% humidified CO2 for 5 days. Then, the outgrown cells from explants were observed under a light microscope (GX53, Olympus, Japan). The experiment was independently repeated three times.
Fibroblast Biocompatibility of 300-PEO-PEOT/PBT
Human foreskin fibroblasts (HFF-1) were acquired from ATCC (Manassas, VA, USA). DMEM glutamax, penicillin (10 U/mL), streptomycin (10 mg/mL), fetal bovine serum (FBS) and sodium pyruvate were ordered from Gibco Invitrogen (Waltham, MA, USA). Calcein AM (1 mg/mL) was purchased from Anaspec (Fremont, CA, USA) and propidium iodine was ordered from Sigma Aldrich (St. Louis, MO, USA). Yellow tetrazolium dye 3-(4,5-dimethyldiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was acquired from Merck Promega (Darmstadt, Germany).
Assessing Cell Viability with MTX Treatment
Cell Viability of MC3T3 Pre-Osteoblasts
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